Method for facilitating the introduction of material into cells
Abstract
The present invention is a method for creating a localized disruption within a boundary of a cell or structure by exposing a boundary of a cell or structure to a set of energetically charged particles while regulating the energy of the charged particles so that the charged particles have an amount of kinetic energy sufficient to create a localized disruption within an area of the boundary of the cell or structure, then upon creation of the localized disruption, the amount of kinetic energy decreases to an amount insufficient to create further damage within the cell or structure beyond the boundary. The present invention is also a method for facilitating the introduction of a material into a cell or structure using the same methodology then further exciting the area of the boundary of the cell or structure where the localized disruption was created so to create a localized temporary opening within the boundary then further introducing the material through the temporary opening into the cell or structure.
- Inventors:
-
- Oak Ridge, TN
- Greenback, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 872991
- Patent Number(s):
- 6060315
- Assignee:
- Lockheed Martin Energy Research Corporation (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC05-96OR22464
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; facilitating; introduction; material; cells; creating; localized; disruption; boundary; cell; structure; exposing; set; energetically; charged; particles; regulating; energy; amount; kinetic; sufficient; create; creation; decreases; insufficient; damage; methodology; exciting; created; temporary; introducing; charged particles; charged particle; kinetic energy; energy sufficient; /435/
Citation Formats
Holcomb, David E, and McKnight, Timothy E. Method for facilitating the introduction of material into cells. United States: N. p., 2000.
Web.
Holcomb, David E, & McKnight, Timothy E. Method for facilitating the introduction of material into cells. United States.
Holcomb, David E, and McKnight, Timothy E. Sat .
"Method for facilitating the introduction of material into cells". United States. https://www.osti.gov/servlets/purl/872991.
@article{osti_872991,
title = {Method for facilitating the introduction of material into cells},
author = {Holcomb, David E and McKnight, Timothy E},
abstractNote = {The present invention is a method for creating a localized disruption within a boundary of a cell or structure by exposing a boundary of a cell or structure to a set of energetically charged particles while regulating the energy of the charged particles so that the charged particles have an amount of kinetic energy sufficient to create a localized disruption within an area of the boundary of the cell or structure, then upon creation of the localized disruption, the amount of kinetic energy decreases to an amount insufficient to create further damage within the cell or structure beyond the boundary. The present invention is also a method for facilitating the introduction of a material into a cell or structure using the same methodology then further exciting the area of the boundary of the cell or structure where the localized disruption was created so to create a localized temporary opening within the boundary then further introducing the material through the temporary opening into the cell or structure.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
Works referenced in this record:
DNA-Mediated Gene Transfer Efficiency Is Enhanced by Ionizing and Ultraviolet Irradiation of Rodent Cells in Vitro: I. Kinetics of Enhancement
journal, November 1985
- Perez, Carl F.; Botchan, Michael R.; Tobias, Cornelius A.
- Radiation Research, Vol. 104, Issue 2
Kinetics of electroinduced pores as a probe of membrane modification produced by ionizing radiation
journal, May 1997
- Petcu, Ileana; Fologea, D.; Radu, M.
- Bioelectrochemistry and Bioenergetics, Vol. 42, Issue 2
Overcoming the Obstacles to Gene Therapy
journal, June 1997
- Friedmann, Theodore
- Scientific American, Vol. 276, Issue 6