Inhibition of selectin binding
Abstract
This invention provides a system for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, this system can be used to palliate certain inflammatory and immunological conditions.
- Inventors:
-
- Rodeo, CA
- Albany, CA
- New Delhi, IN
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 872565
- Patent Number(s):
- 5962422
- Application Number:
- 08/807428
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- inhibition; selectin; binding; provides; inhibiting; cells; expressing; p-; l-selectin; surface; corresponding; ligand; covalently; crosslinked; lipid; composition; prepared; saccharides; acidic; separate; lipids; interposed; inhibit; achieved; effective; oligosaccharide; concentration; 10; fold; below; free; saccharide; selectins; involved; recruiting; sites; injury; palliate; inflammatory; immunological; conditions; recruiting cells; selectin binding; separate lipids; oligosaccharide concentration; expressing p-; fold below; free saccharide; lipid composition; effective oligosaccharide; covalently crosslinked; crosslinked lipid; corresponding ligand; immunological conditions; inhibit binding; /514/435/
Citation Formats
Nagy, Jon O, Spevak, Wayne R, Dasgupta, Falguni, and Bertozzi, Carolyn. Inhibition of selectin binding. United States: N. p., 1999.
Web.
Nagy, Jon O, Spevak, Wayne R, Dasgupta, Falguni, & Bertozzi, Carolyn. Inhibition of selectin binding. United States.
Nagy, Jon O, Spevak, Wayne R, Dasgupta, Falguni, and Bertozzi, Carolyn. Fri .
"Inhibition of selectin binding". United States. https://www.osti.gov/servlets/purl/872565.
@article{osti_872565,
title = {Inhibition of selectin binding},
author = {Nagy, Jon O and Spevak, Wayne R and Dasgupta, Falguni and Bertozzi, Carolyn},
abstractNote = {This invention provides a system for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, this system can be used to palliate certain inflammatory and immunological conditions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
A plasmid facilitating in vitro construction of phoA gene fusions in Escherichia coli
journal, January 1989
- Gutierrez, Claude; Devedjian, Jean Christophe
- Nucleic Acids Research, Vol. 17, Issue 10
Characterization of a Calcium Porter of Streptococcus Pneumoniae Involved In Calcium Regulation of Growth and Competence
journal, March 1993
- Trombe, M. -C.
- Journal of General Microbiology, Vol. 139, Issue 3
The mgtB Mg2+ transport locus of Salmonella typhimurium encodes a P-type ATPase.
journal, January 1991
- Snavely, M. D.; Miller, C. G.; Maguire, M. E.
- Journal of Biological Chemistry, Vol. 266, Issue 2
Genetic immunization is a simple method for eliciting an immune response
journal, March 1992
- Tang, De-chu; DeVit, Michael; Johnston, Stephen A.
- Nature, Vol. 356, Issue 6365
Secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-haemolysin bifunctional protein of Bordetella pertussis.
journal, December 1988
- Glaser, P.; Sakamoto, H.; Bellalou, J.
- The EMBO Journal, Vol. 7, Issue 12
Evidence for high affinity binding-protein dependent transport systems in gram-positive bacteria and in Mycoplasma.
journal, December 1988
- Gilson, E.; Alloing, G.; Schmidt, T.
- The EMBO Journal, Vol. 7, Issue 12
Construction and properties of a new insertion vector, pJDC9, that is protected by transcriptional terminators and useful for cloning of DNA from Streptococcus pneumoniae
journal, April 1988
- Chen, Jau-Der; Morrison, Donald A.
- Gene, Vol. 64, Issue 1
A sandwich adhesin on Streptococcus pneumoniae attaching to human oropharyngeal epithelial cells in vitro
journal, April 1988
- Andersson, B.; Beachey, E. H.; Tomasz, A.
- Microbial Pathogenesis, Vol. 4, Issue 4
Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene.
journal, January 1992
- Yother, J.; Handsome, G. L.; Briles, D. E.
- Journal of Bacteriology, Vol. 174, Issue 2
Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.
journal, December 1988
- Taha, M. K.; So, M.; Seifert, H. S.
- The EMBO Journal, Vol. 7, Issue 13
The ami locus of the Gram-positive bacterium Streptococcus pneumoniae is similar to binding protein-dependent transport operons of Gram-negative bacteria
journal, April 1990
- Alloing, G.; Trombe, M. C.; Claverys, J. P.
- Molecular Microbiology, Vol. 4, Issue 4
Adherence, coaggregation, and hydrophobicity of Streptococcus gordonii associated with expression of cell surface lipoproteins.
journal, January 1992
- Jenkinson, H. F.
- Infection and Immunity, Vol. 60, Issue 3
Bacterial Adherence: Adhesin-Receptor Interactions Mediating the Attachment of Bacteria to Mucosal Surfaces
journal, March 1981
- Beachey, E. H.
- Journal of Infectious Diseases, Vol. 143, Issue 3
Genetic identification of exported proteins in Streptococcus pneumoniae
journal, September 1993
- Pearce, B. J.; Yin, Y. B.; Masure, H. R.
- Molecular Microbiology, Vol. 9, Issue 5
D-E-A-D protein family of putative RNA helicases
journal, February 1992
- Schmid, S. R.; Linder, P.
- Molecular Microbiology, Vol. 6, Issue 3
Role of pilA, an essential regulatory gene of Neisseria gonorrhoeae, in the stress response.
journal, January 1992
- Taha, M. K.; Larribe, M.; Dupuy, B.
- Journal of Bacteriology, Vol. 174, Issue 18
TnphoA: a transposon probe for protein export signals.
journal, December 1985
- Manoil, C.; Beckwith, J.
- Proceedings of the National Academy of Sciences, Vol. 82, Issue 23
Consequences of microbial attachment: directing host cell functions with adhesins.
journal, January 1992
- Hoepelman, A. I.; Tuomanen, E. I.
- Infection and Immunity, Vol. 60, Issue 5
Peptide permeases modulate transformation in Streptococcus pneumoniae
journal, June 1994
- Pearce, B. J.; Naughton, A. M.; Masure, H. R.
- Molecular Microbiology, Vol. 12, Issue 6
Cloning of the amiA locus of Streptococcus pneumoniae and identification of its functional limits
journal, March 1989
- Alloing, Geneviéve; Trombe, Marie-Claude; Claverys, Jean-Pierre
- Gene, Vol. 76, Issue 2
Analysis of pneumococcal PspA microheterogeneity in SDS polyacrylamide gels and the association of PspA with the cell membrane
journal, November 1992
- Talkington, Deborah F.; Voellinger, David C.; Mcdaniel, Larry S.
- Microbial Pathogenesis, Vol. 13, Issue 5
The Clp proteins: proteolysis regulators or molecular chaperones?
journal, January 1992
- Squires, C.; Squires, C. L.
- Journal of Bacteriology, Vol. 174, Issue 4
Construction and use of signal sequence selection vectors in Escherichia coli and Bacillus subtilis.
journal, January 1987
- Smith, H.; Bron, S.; Van Ee, J.
- Journal of Bacteriology, Vol. 169, Issue 7
Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function.
journal, January 1993
- Ruhfel, R. E.; Manias, D. A.; Dunny, G. M.
- Journal of Bacteriology, Vol. 175, Issue 16
The cell wall mediates pneumococcal attachment to and cytopathology in human endothelial cells.
journal, January 1993
- Geelen, S.; Bhattacharyya, C.; Tuomanen, E.
- Infection and Immunity, Vol. 61, Issue 4
The glucose permease of the phosphotransferase system of Bacillus subtilis: evidence for II Glc and III Glc domains
journal, May 1991
- Gonzy-Tréboul, G.; Waard, J. H.; Zagorec, M.
- Molecular Microbiology, Vol. 5, Issue 5
Marker Discrimination in Transformation and Mutation of Pneumococcus
journal, December 1973
- Tiraby, J. - G.; Fox, M. S.
- Proceedings of the National Academy of Sciences, Vol. 70, Issue 12
Nucleotide sequences of genes encoding penicillin-binding proteins from Streptococcus pneumoniae and Streptococcus oralis with high homology to Escherichia coli penicillin-binding proteins 1a and 1b.
journal, January 1992
- Martin, C.; Briese, T.; Hakenbeck, R.
- Journal of Bacteriology, Vol. 174, Issue 13
The calmodulin-sensitive adenylate cyclase of Bordetella pertussis : cloning and expression in Escherichia col
journal, January 1988
- Glaser, P.; Ladant, D.; Sezer, O.
- Molecular Microbiology, Vol. 2, Issue 1
Characterization of signal-sequence-coding regions selected from the Bacillus subtilis chromosome
journal, October 1988
- Smith, Hilde; de Jong, Anne; Bron, Sierd
- Gene, Vol. 70, Issue 2
Gene inoculation generates immune responses against human immunodeficiency virus type 1.
journal, May 1993
- Wang, B.; Ugen, K. E.; Srikantan, V.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 9
Protein export elements from Lactococcus lactis
journal, September 1992
- Perez-Martinez, Gaspar; Kok, Jan; Venema, Gerard
- Molecular and General Genetics MGG, Vol. 234, Issue 3
Relatedness of penicillin-binding protein 1a genes from different clones of penicillin-resistant Streptococcus pneumoniae isolated in South Africa and Spain.
journal, November 1992
- Martin, C.; Sibold, C.; Hakenbeck, R.
- The EMBO Journal, Vol. 11, Issue 11