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Title: Quantitative DNA fiber mapping

Abstract

The present invention relates generally to the DNA mapping and sequencing technologies. In particular, the present invention provides enhanced methods and compositions for the physical mapping and positional cloning of genomic DNA. The present invention also provides a useful analytical technique to directly map cloned DNA sequences onto individual stretched DNA molecules.

Inventors:
 [1];  [2]
  1. San Francisco, CA
  2. Oakland, CA
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
OSTI Identifier:
872053
Patent Number(s):
5851769
Assignee:
Regents of University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
quantitative; dna; fiber; mapping; relates; sequencing; technologies; particular; provides; enhanced; methods; compositions; physical; positional; cloning; genomic; useful; analytical; technique; directly; map; cloned; sequences; individual; stretched; molecules; dna sequences; dna sequence; dna molecules; cloned dna; dna molecule; provides enhanced; enhanced method; physical mapping; analytical technique; /435/

Citation Formats

Gray, Joe W, and Weier, Heinz-Ulrich G. Quantitative DNA fiber mapping. United States: N. p., 1998. Web.
Gray, Joe W, & Weier, Heinz-Ulrich G. Quantitative DNA fiber mapping. United States.
Gray, Joe W, and Weier, Heinz-Ulrich G. Thu . "Quantitative DNA fiber mapping". United States. https://www.osti.gov/servlets/purl/872053.
@article{osti_872053,
title = {Quantitative DNA fiber mapping},
author = {Gray, Joe W and Weier, Heinz-Ulrich G},
abstractNote = {The present invention relates generally to the DNA mapping and sequencing technologies. In particular, the present invention provides enhanced methods and compositions for the physical mapping and positional cloning of genomic DNA. The present invention also provides a useful analytical technique to directly map cloned DNA sequences onto individual stretched DNA molecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1998},
month = {Thu Jan 01 00:00:00 EST 1998}
}

Works referenced in this record:

Rapid identification of yeast artificial chromosome clones by matrix pooling and crude Iysate PCR
journal, January 1990


Generation of Five High-Complexity Painting Probe Libraries from Flow-Sorted Mouse Chromosomes
journal, June 1994


Cytogenetic analysis by chromosome painting
journal, March 1994


A high resolution physical map of 2.5 Mbp of the Down syndrome region on chromosome 21
journal, January 1994


Alignment and sensitive detection of DNA by a moving interface
journal, September 1994


Evaluation of a cosmid contig physical map of human chromosome 16
journal, August 1992


Continuum of overlapping clones spanning the entire human chromosome 21q
journal, October 1992


A physical map of the human Y-chromosome short arm
journal, August 1990


A new five-year plan for the U.S. Human Genome Project
journal, October 1993


Construction and characterization of band-specific DNA libraries
journal, May 1990


High resolution ordering of YAC contigs using extended chromatin and chromosomes
journal, January 1994


Synthesis of Y chromosome-specific labeled DNA probes by in vitro DNA amplification.
journal, March 1990


High-resolution in situ hybridization using DNA halo preparations
journal, January 1992


Transposon-facilitated DNA sequencing.
journal, February 1991


An integrated YAC-overlap and ‘cosmid-pocket’ map of the human chromosome 21
journal, January 1994


Semiautomated DNA probe mapping using digital imaging microscopy: II. System performance
journal, January 1995


Semiautomated DNA probe mapping using digital imaging microscopy: I. System development
journal, January 1995


Preparation of immobilized proteins covalently coupled through silane coupling agents to inorganic supports
journal, June 1993


Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds
journal, May 1988


Applications of polymerase chain reaction methods in genome mapping
journal, June 1991


PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants
journal, January 1994


Physical and genetic map of 5q31: use of fluorescence in situ hybridization data to identify errors in the CEPH database
journal, January 1994


Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model
journal, September 1992


Mapping the whole human genome by fingerprinting yeast artificial chromosomes
journal, September 1992


Current methods for YAC clone characterization
journal, September 1990


Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification
journal, March 1989


A new system for high-resolution DNA sequence mapping in interphase pronuclei
journal, May 1991


Identification of CpG islands in a physical map encompassing the Friedreich's ataxia locus
journal, January 1991


Physical and Genetic Maps for Chromosome 10
journal, May 1993


New RNA Polymerase from Escherichia coli infected with Bacteriophage T7
journal, October 1970


A radiation hybrid map of the region on human chromosome 22 containing the neurofibromatosis type 2 locus
journal, November 1992


A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy.
journal, March 1992


Use of reference libraries and hybridisation fingerprinting for relational genome analysis
journal, June 1993


High-resolution DNA Fiber-FISH for genomic DNA mapping and colour bar-coding of large genes
journal, May 1995


FISH in genome research and molecular diagnostics
journal, June 1995


PCR amplification and analysis of yeast artificial chromosomes
journal, August 1992


The human genome project.
journal, May 1993


A Replicating RNA Molecule Suitable for a Detailed Analysis of Extracellular Evolution and Replication
journal, October 1972


High resolution visual mapping of stretched DNA by fluorescent hybridization
journal, September 1993


Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
journal, October 1992


Current trends in mapping human genes
journal, January 1991


A second-generation linkage map of the human genome
journal, October 1992


Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction.
journal, February 1990


Cytogenetic analysis by chromosome painting using dop-pcr amplified flow-sorted chromosomes
journal, April 1992


Recently transposed Alu repeats result from multiple source genes
journal, January 1990


A first-generation physical map of the human genome
journal, December 1993


Extending the capabilities of interphase chromatin mapping
journal, November 1992


Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.
journal, September 1992


Amplification of 20q13 locus in breast cancer: -Defining the minimal region
journal, October 1994


Rapid generation of region specific probes by chromosome microdissection and their application
journal, April 1992


Assembly and analysis of cosmid contigs in the CEA-gene family region of human chromosome 19
journal, January 1992


Preparation and screening of an arrayed human genomic library generated with the P1 cloning system.
journal, March 1994


Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.
journal, June 1990


Construction of a YAC contig and a STS map spanning at least seven megabasepairs in chromosome 5q34-35
journal, January 1994