Method for increasing thermostability in cellulase ennzymes
Abstract
The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.
- Inventors:
-
- Golden, CO
- Denver, CO
- Littleton, CO
- Wheat Ridge, CO
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- OSTI Identifier:
- 871345
- Patent Number(s):
- 5712142
- Assignee:
- Midwest Research Institute (Kansas City, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC36-83CH10093
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; increasing; thermostability; cellulase; ennzymes; encoding; acidothermus; cellulolyticus; e1; endoglucanase; cloned; expressed; pichia; pastoris; modified; enzyme; comprising; catalytic; domain; size; demonstrates; enhanced; produced; methods; producing; proteolytic; cleavage; remove; cellulose; binding; linker; peptide; genetic; truncation; expression; product; e1 endoglucanase; binding domain; cellulose binding; acidothermus cellulolyticus; modified e1; catalytic domain; endoglucanase enzyme; encoding acidothermus; cellulolyticus e1; /435/999/
Citation Formats
Adney, William S, Thomas, Steven R, Baker, John O, Himmel, Michael E, and Chou, Yat-Chen. Method for increasing thermostability in cellulase ennzymes. United States: N. p., 1998.
Web.
Adney, William S, Thomas, Steven R, Baker, John O, Himmel, Michael E, & Chou, Yat-Chen. Method for increasing thermostability in cellulase ennzymes. United States.
Adney, William S, Thomas, Steven R, Baker, John O, Himmel, Michael E, and Chou, Yat-Chen. Tue .
"Method for increasing thermostability in cellulase ennzymes". United States. https://www.osti.gov/servlets/purl/871345.
@article{osti_871345,
title = {Method for increasing thermostability in cellulase ennzymes},
author = {Adney, William S and Thomas, Steven R and Baker, John O and Himmel, Michael E and Chou, Yat-Chen},
abstractNote = {The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 27 00:00:00 EST 1998},
month = {Tue Jan 27 00:00:00 EST 1998}
}
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Works referenced in this record:
Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
journal, July 1986
- MOHAGHEGHI, A.; GROHMANN, K.; HIMMEL, M.
- International Journal of Systematic Bacteriology, Vol. 36, Issue 3, p. 435-443