Subtilisin variants suitable for hydrolysis and synthesis in organic media

Arnold, Frances H; Chen, Keqin

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Title: Subtilisin variants suitable for hydrolysis and synthesis in organic media

Abstract

In accordance with the present invention, there are provided novel, modified subtilisin enzyme(s) having improved catalytic activity and/or stability in organic media.

Inventors:

Arnold, Frances H ^[1]; Chen, Keqin ^[1]

Pasadena, CA

Issue Date:: Tue May 31 00:00:00 EDT 1994

Research Org.:: Department of Energy (Advanced Industrial Concepts Program)

OSTI Identifier:: 869332

Patent Number(s):: 5316935

Application Number:: 07/864,298

Assignee:: California Institute of Technology (Pasadena, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/54 - bacteria being Bacillus

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DOE Contract Number:: 66766

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: subtilisin; variants; suitable; hydrolysis; synthesis; organic; media; accordance; provided; novel; modified; enzyme; improved; catalytic; activity; stability; catalytic activity; organic media; improved catalytic; /435/999/

Citation Formats


                    Arnold, Frances H, and Chen, Keqin. Subtilisin variants suitable for hydrolysis and synthesis in organic media.  United States: N. p., 1994. 
        Web.

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                    Arnold, Frances H, & Chen, Keqin. Subtilisin variants suitable for hydrolysis and synthesis in organic media.  United States.

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                    Arnold, Frances H, and Chen, Keqin. Tue .  
        "Subtilisin variants suitable for hydrolysis and synthesis in organic media".  United States.  https://www.osti.gov/servlets/purl/869332.

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@article{osti_869332,

  title        = {Subtilisin variants suitable for hydrolysis and synthesis in organic media},

  author       = {Arnold, Frances H and Chen, Keqin},

  abstractNote = {In accordance with the present invention, there are provided novel, modified subtilisin enzyme(s) having improved catalytic activity and/or stability in organic media.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue May 31 00:00:00 EDT 1994},

  month        = {Tue May 31 00:00:00 EDT 1994}

}

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Works referenced in this record:

Organic solvents strip water off enzymes
journal, February 1992

Gorman, Lu Ann S.; Dordick, Jonathan S.
Biotechnology and Bioengineering, Vol. 39, Issue 4
https://doi.org/10.1002/bit.260390405

Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases
journal, April 1985

Meloun, Bedřich; Baudyš, Miroslav; Kostka, Vladimïr
FEBS Letters, Vol. 183, Issue 2
https://doi.org/10.1016/0014-5793(85)80775-4

Surface charge substitutions increase the stability of .alpha.-lytic protease in organic solvents
journal, July 1991

Martinez, Pascal; Arnold, Frances H.
Journal of the American Chemical Society, Vol. 113, Issue 16
https://doi.org/10.1021/ja00016a096

Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases
journal, January 1991

Siezen, Roland J.; de Vos, William M.; Leunissen, Jack A. M.
"Protein Engineering, Design and Selection", Vol. 4, Issue 7
https://doi.org/10.1093/protein/4.7.719

Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering
journal, February 1987

Russell, Alan J.; Thomas, Paul G.; Fersht, Alan R.
Journal of Molecular Biology, Vol. 193, Issue 4
https://doi.org/10.1016/0022-2836(87)90360-3

The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
journal, June 1988

Bott, R.; Ultsch, M.; Kossiakoff, A.
Journal of Biological Chemistry, Vol. 263, Issue 16
https://doi.org/10.1016/S0021-9258(18)68582-5

Effects of pH and urea on the conformational properties of subtilisin DY
journal, November 1982

Ricchelli, F.; Jori, G.; Filippi, B.
Biochemical Journal, Vol. 207, Issue 2
https://doi.org/10.1042/bj2070201

Enzyme function in organic solvents
journal, January 1992

Gupta, Munishwar N.
European Journal of Biochemistry, Vol. 203, Issue 1-2
https://doi.org/10.1111/j.1432-1033.1992.tb19823.x

Engineering enzymes for non-aqueous solvents
journal, January 1990

Arnold, F.
Trends in Biotechnology, Vol. 8
https://doi.org/10.1016/0167-7799(90)90186-2

Enzyme engineering for nonaqueous solvents. II. Additive effects of mutations on the stability and activity of subtilisin E in polar organic media
journal, March 1991

Chen, Keqin; Robinson, Amy C.; Van Dam, Mariana E.
Biotechnology Progress, Vol. 7, Issue 2
https://doi.org/10.1021/bp00008a007

Enzyme Engineering for Nonaqueous Solvents: Random Mutagenesis to Enhance Activity of Subtilisin E in Polar Organic Media
journal, November 1991

Chen, Keqin; Arnold, Frances H.
Bio/Technology, Vol. 9, Issue 11
https://doi.org/10.1038/nbt1191-1073

Hydrophilicity of polar amino acid side-chains is markedly reduced by flanking peptide bonds
journal, April 1988

Roseman, Mark A.
Journal of Molecular Biology, Vol. 200, Issue 3
https://doi.org/10.1016/0022-2836(88)90540-2

Stabilization of substilisin E in organic solvents by site-directed mutagenesis
journal, January 1992

Martinez, Pascal; Van Dam, Mariana E.; Robinson, Amy C.
Biotechnology and Bioengineering, Vol. 39, Issue 2
https://doi.org/10.1002/bit.260390204

Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E
journal, March 1992

Economou, Chariklia; Chen, Keqin; Arnold, Frances H.
Biotechnology and Bioengineering, Vol. 39, Issue 6
https://doi.org/10.1002/bit.260390610

Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations
journal, January 1990

Wong, Chi Huey; Chen, S. T.; Hennen, William J.
Journal of the American Chemical Society, Vol. 112, Issue 3
https://doi.org/10.1021/ja00159a006

Engineering subtilisin for reaction in dimethylformamide
journal, January 1991

Zhong, Ziyang; Liu, Jennifer L. C.; Dinterman, Lois M.
Journal of the American Chemical Society, Vol. 113, Issue 2
https://doi.org/10.1021/ja00002a051

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Similar Records

Title: Subtilisin variants suitable for hydrolysis and synthesis in organic media

Abstract

Citation Formats

Organic solvents strip water off enzymes journal, February 1992

Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases journal, April 1985

Surface charge substitutions increase the stability of .alpha.-lytic protease in organic solvents journal, July 1991

Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases journal, January 1991

Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering journal, February 1987

The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation. journal, June 1988

Effects of pH and urea on the conformational properties of subtilisin DY journal, November 1982

Enzyme function in organic solvents journal, January 1992

Engineering enzymes for non-aqueous solvents journal, January 1990

Enzyme engineering for nonaqueous solvents. II. Additive effects of mutations on the stability and activity of subtilisin E in polar organic media journal, March 1991

Enzyme Engineering for Nonaqueous Solvents: Random Mutagenesis to Enhance Activity of Subtilisin E in Polar Organic Media journal, November 1991

Hydrophilicity of polar amino acid side-chains is markedly reduced by flanking peptide bonds journal, April 1988

Stabilization of substilisin E in organic solvents by site-directed mutagenesis journal, January 1992

Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E journal, March 1992

Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations journal, January 1990

Engineering subtilisin for reaction in dimethylformamide journal, January 1991

Organic solvents strip water off enzymes
journal, February 1992

Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases
journal, April 1985

Surface charge substitutions increase the stability of .alpha.-lytic protease in organic solvents
journal, July 1991

Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases
journal, January 1991

Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering
journal, February 1987

The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
journal, June 1988

Effects of pH and urea on the conformational properties of subtilisin DY
journal, November 1982

Enzyme function in organic solvents
journal, January 1992

Engineering enzymes for non-aqueous solvents
journal, January 1990

Enzyme engineering for nonaqueous solvents. II. Additive effects of mutations on the stability and activity of subtilisin E in polar organic media
journal, March 1991

Enzyme Engineering for Nonaqueous Solvents: Random Mutagenesis to Enhance Activity of Subtilisin E in Polar Organic Media
journal, November 1991

Hydrophilicity of polar amino acid side-chains is markedly reduced by flanking peptide bonds
journal, April 1988

Stabilization of substilisin E in organic solvents by site-directed mutagenesis
journal, January 1992

Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E
journal, March 1992

Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations
journal, January 1990

Engineering subtilisin for reaction in dimethylformamide
journal, January 1991