Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068

Himmel, Michael E; Adney, William S; Tucker, Melvin P; Grohmann, Karel

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Title: Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068

Abstract

A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068). The cellulase is water soluble, possesses both C.sub.1 and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83.degree. C. at pH's from about 2 to about 9, and in inactivation temperature of about 110.degree. C. at pH's from about 2 to about 9.

Inventors:

Himmel, Michael E ^[1]; Adney, William S ^[2]; Tucker, Melvin P ^[3]; Grohmann, Karel ^[4]

Littleton, CO
Golden, CO
Lakewood, CO
Winter Haven, FL

Issue Date:: Sat Jan 01 00:00:00 EST 1994

Research Org.:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Midwest Research Institute, Kansas City, MO (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 869113

Patent Number(s):: 5275944

Assignee:: Midwest Research Institute (Kansas City, MO)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K2319/02 - containing a signal sequence

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y302/01004 - Cellulase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/814 - Enzyme separation or purification
Y10S435/822 - using bacteria or actinomycetales

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DOE Contract Number:: AC02-83CH10093

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: thermostable; purified; endoglucanas; acidothermus; cellulolyticus; atcc; 43068; molecular; weight; cellulase; endoglucanase; 57; 420; 74; 580; daltons; water; soluble; possesses; types; enzyme; activity; degree; stability; heat; exhibits; optimum; temperature; 83; ph; inactivation; 110; cellulolyticus atcc; optimum temperature; enzyme activity; water soluble; molecular weight; acidothermus cellulolyticus; thermostable purified; temperature activity; weight cellulase; inactivation temperature; purified endoglucanas; /435/

Citation Formats


                    Himmel, Michael E, Adney, William S, Tucker, Melvin P, and Grohmann, Karel. Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068.  United States: N. p., 1994. 
        Web.

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                    Himmel, Michael E, Adney, William S, Tucker, Melvin P, & Grohmann, Karel. Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068.  United States.

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                    Himmel, Michael E, Adney, William S, Tucker, Melvin P, and Grohmann, Karel. Sat .  
        "Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068".  United States.  https://www.osti.gov/servlets/purl/869113.

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@article{osti_869113,

  title        = {Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068},

  author       = {Himmel, Michael E and Adney, William S and Tucker, Melvin P and Grohmann, Karel},

  abstractNote = {A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068). The cellulase is water soluble, possesses both C.sub.1 and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83.degree. C. at pH's from about 2 to about 9, and in inactivation temperature of about 110.degree. C. at pH's from about 2 to about 9.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sat Jan 01 00:00:00 EST 1994},

  month        = {Sat Jan 01 00:00:00 EST 1994}

}

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Works referenced in this record:

Bacterium produces thermostable cellulase
journal, May 1987

Seltze, Richard
Chemical & Engineering News Archive, Vol. 65, Issue 18
https://doi.org/10.1021/cen-v065n018.p023

Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases
journal, August 1989

Tucker, Melvin P.; Mohagheghi, Ali; Grohmann, Karel
Nature Biotechnology, Vol. 7, Issue 8
https://doi.org/10.1038/nbt0889-817

The Production of Cellulases
book, June 1969

Mandels, Mary; Weber, James
Advances in Chemistry
https://doi.org/10.1021/ba-1969-0095.ch023

Cellulolytic enzyme system of Acetivibrio cellulolyticus
journal, March 1981

Saddler, J. N.; Khan, A. W.
Canadian Journal of Microbiology, Vol. 27, Issue 3
https://doi.org/10.1139/m81-045

Mechanisms of Thermophily
journal, January 1978

Amelunxen, Remi E.; Murdock, Archie L.; Welker, Neil E.
CRC Critical Reviews in Microbiology, Vol. 6, Issue 4
https://doi.org/10.3109/10408417809090626

Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
journal, July 1986

MOHAGHEGHI, A.; GROHMANN, K.; HIMMEL, M.
International Journal of Systematic Bacteriology, Vol. 36, Issue 3, p. 435-443
https://doi.org/10.1099/00207713-36-3-435

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Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068

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A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068) is presented. The cellulase is water soluble, possesses both C[sub 1] and C[sub x] types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83 C at pH's from about 2 to about 9, and in inactivation temperature of about 110 C at pH's from about 2 to about 9. 7 figures.
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A purified low molecular weight endoglucanase II from Acidothermus cellulolyticus (ATCC 43068) is disclosed. The endoglucanase is water soluble, possesses both C.sub.1, and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 81.degree. C. at pH's from about 2 to about 9, and at a inactivation temperature of about 100.degree. C. at pH's from about 2 to about 9.
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A substantially purified high molecular weight cellulase enzyme having a molecular weight of between about 156,000 to about 203,400 daltons isolated from the bacterium Acidothermus cellulolyticus (ATCC 43068) and a method of producing it are disclosed. The enzyme is water soluble, possesses both C.sub.1 and C.sub.x types of enzymatic activity, has a high degree of stability toward heat and exhibits both a high optimum temperature activity and high inactivation characteristics.
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Similar Records

Title: Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068

Abstract

Citation Formats

Bacterium produces thermostable cellulase journal, May 1987

Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases journal, August 1989

The Production of Cellulases book, June 1969

Cellulolytic enzyme system of Acetivibrio cellulolyticus journal, March 1981

Mechanisms of Thermophily journal, January 1978

Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria journal, July 1986

Bacterium produces thermostable cellulase
journal, May 1987

Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases
journal, August 1989

The Production of Cellulases
book, June 1969

Cellulolytic enzyme system of Acetivibrio cellulolyticus
journal, March 1981

Mechanisms of Thermophily
journal, January 1978

Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
journal, July 1986