Rotor assembly and assay method
Abstract
A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor.
- Inventors:
-
- Oak Ridge, TN
- Loudon, TN
- Knoxville, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 868912
- Patent Number(s):
- 5242803
- Assignee:
- Martin Marietta Energy Systems, Inc. (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B - PERFORMING OPERATIONS B04 - CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES B04B - CENTRIFUGES
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- rotor; assembly; assay; method; carrying; rotatable; axis; rotation; central; chamber; third; fourth; fifth; sixth; chambers; communication; radiate; shuttle; movable; insertable; including; reaction; cup; immobilized; antigen; antibody; transport; moving; six; passing; centrifugation; steps; perform; separating; plasma; blood; cells; binding; washing; drying; enzyme; conjugate; reacting; substrate; optically; comparing; resulting; product; unreacted; solution; movement; provided; attaching; magnet; supplying; magnetic; field; central chamber; rotor assembly; blood cells; magnetic field; reaction product; resulting reaction; blood cell; assay method; /435/422/436/
Citation Formats
Burtis, Carl A, Johnson, Wayne F, and Walker, William A. Rotor assembly and assay method. United States: N. p., 1993.
Web.
Burtis, Carl A, Johnson, Wayne F, & Walker, William A. Rotor assembly and assay method. United States.
Burtis, Carl A, Johnson, Wayne F, and Walker, William A. Fri .
"Rotor assembly and assay method". United States. https://www.osti.gov/servlets/purl/868912.
@article{osti_868912,
title = {Rotor assembly and assay method},
author = {Burtis, Carl A and Johnson, Wayne F and Walker, William A},
abstractNote = {A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1993},
month = {Fri Jan 01 00:00:00 EST 1993}
}
Works referenced in this record:
Two-dimensional centrifugation for desk-top clinical chemistry.
journal, September 1985
- Schultz, S. G.; Holen, J. T.; Donohue, J. P.
- Clinical Chemistry, Vol. 31, Issue 9
Development of a simple device for processing whole-blood samples into measured aliquots of plasma.
journal, September 1986
- Burtis, C. A.; Johnson, W. F.; Walker, W. A.
- Clinical Chemistry, Vol. 32, Issue 9