Detection and differentiation of coxiella burnetii in biological fluids
Abstract
Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.
- Inventors:
-
- Richland, WA
- Moscow, ID
- Albuquerque, NM
- Pullman, WA
- Issue Date:
- Research Org.:
- Battelle Memorial Institute, Columbus, OH (United States)
- OSTI Identifier:
- 867154
- Patent Number(s):
- 4876186
- Assignee:
- Battelle Development Corporation (Columbus, OH)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- detection; differentiation; coxiella; burnetii; biological; fluids; methods; detecting; presence; samples; method; differentiating; strains; capable; causing; acute; disease; chronic; disclosed; comprise; treating; cells; contained; sample; expose; cellular; dna; hybridizing; specifically; rickettsial; burnetii-specific; labeled; probe; radioisotope; biotin; labels; preferred; allowing; autoradiography; colorimetric; assays; respectively; biological samples; labeled dna; cellular dna; biological fluids; treating cells; strains capable; biological fluid; comprise treating; acute disease; causing acute; causing chronic; colorimetric assays; coxiella burnetii; biological sample; cells contained; chronic disease; colorimetric assay; dna probe; expose cellular; differentiating strains; cells contain; /435/436/999/
Citation Formats
Frazier, Marvin E, Mallavia, Louis P, Baca, Oswald G, and Samuel, James E. Detection and differentiation of coxiella burnetii in biological fluids. United States: N. p., 1989.
Web.
Frazier, Marvin E, Mallavia, Louis P, Baca, Oswald G, & Samuel, James E. Detection and differentiation of coxiella burnetii in biological fluids. United States.
Frazier, Marvin E, Mallavia, Louis P, Baca, Oswald G, and Samuel, James E. Sun .
"Detection and differentiation of coxiella burnetii in biological fluids". United States. https://www.osti.gov/servlets/purl/867154.
@article{osti_867154,
title = {Detection and differentiation of coxiella burnetii in biological fluids},
author = {Frazier, Marvin E and Mallavia, Louis P and Baca, Oswald G and Samuel, James E},
abstractNote = {Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}
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