Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds
Abstract
Apparatus and method for electronically reading planar two dimensional .beta.-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge of the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the .beta.-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700 .mu.m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally bemore »
- Inventors:
-
- Santa Fe, NM
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 866329
- Patent Number(s):
- 4686368
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G01 - MEASURING G01T - MEASUREMENT OF NUCLEAR OR X-RADIATION
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- apparatus; method; reading; two-dimensional; electrophoretograms; containing; beta; -ray-emitting; labeled; compounds; electronically; planar; dimensional; -ray; emitter-labeled; single; flat; rectangular; multiwire; proportional; chamber; placed; close; proximity; assembly; intense; uniform; magnetic; field; disposed; perpendicular; manner; rays; emitted; direction; caused; execute; helical; motions; substantially; preserve; knowledge; coordinates; origin; perpendicularly; oriented; parallel; wire; plane; cathodes; sense; location; -rays; ionization; generated; detection; gas; coupled; electron; avalanche; effect; resulting; action; anode; located; therebetween; scintillator; permits; rendered; insensitive; signals; cosmic; incident; resolution; concentrations; radioactive; exceeds; 700; represent; significant; improvement; conventional; autoradiographic; techniques; dynamic; range; linearity; sensitivity; data; collection; concentration; position; map; highly; labeling; nuclides; obtained; hour; uniform magnetic; beta rays; wire anode; dynamic range; close proximity; magnetic field; data collection; electron avalanche; radioactive compound; beta ray; labeled compounds; located therebetween; parallel wire; perpendicular manner; active compound; rays emitted; significant improvement; cosmic rays; significant concentration; rays incident; /250/
Citation Formats
Anderson, Herbert L, Kinnison, W Wayne, and Lillberg, John W. Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds. United States: N. p., 1987.
Web.
Anderson, Herbert L, Kinnison, W Wayne, & Lillberg, John W. Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds. United States.
Anderson, Herbert L, Kinnison, W Wayne, and Lillberg, John W. Thu .
"Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds". United States. https://www.osti.gov/servlets/purl/866329.
@article{osti_866329,
title = {Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds},
author = {Anderson, Herbert L and Kinnison, W Wayne and Lillberg, John W},
abstractNote = {Apparatus and method for electronically reading planar two dimensional .beta.-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge of the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the .beta.-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700 .mu.m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally be obtained in less than one hour.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}