Apparatus for eliminating background interference in fluorescence measurements
Abstract
The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle.
- Inventors:
-
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 865781
- Patent Number(s):
- 4573796
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- apparatus; eliminating; background; interference; fluorescence; measurements; disclosure; directed; multiple; laser; flow; cytometer; biological; particle; stained; fluorescent; dyes; excited; detector; detects; scatters; light; gate; signal; generated; delayed; reaches; excites; component; biological particle; flow cytometer; fluorescent dyes; detector detects; fluorescent dye; multiple laser; fluorescence detector; fluorescence measurements; fluorescence measurement; eliminating background; background interference; delayed signal; /356/250/
Citation Formats
Martin, John C, and Jett, James H. Apparatus for eliminating background interference in fluorescence measurements. United States: N. p., 1986.
Web.
Martin, John C, & Jett, James H. Apparatus for eliminating background interference in fluorescence measurements. United States.
Martin, John C, and Jett, James H. Wed .
"Apparatus for eliminating background interference in fluorescence measurements". United States. https://www.osti.gov/servlets/purl/865781.
@article{osti_865781,
title = {Apparatus for eliminating background interference in fluorescence measurements},
author = {Martin, John C and Jett, James H},
abstractNote = {The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}
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