Assay for mutagenesis in heterozygous diploid human lymphoblasts
Abstract
An assay is disclosed for determining mutagenic damage caused by the administration of a known or suspected mutagen to diploid human lymphoblastoid cell lines. The gene locus employed for this assay is the gene for thymidine kinase, uridine kinase, or cytidine deaminase. Since human lymphoblastoid cells contain two genes for these enzymes, heterozygotes of human lymphoblastoid cells are used in this assay.
- Inventors:
-
- Somerville, MA
- Brookline, MA
- Cambridge, MA
- (Arlington, MA)
- Issue Date:
- OSTI Identifier:
- 864055
- Patent Number(s):
- 4302535
- Assignee:
- Massachusetts Institute of Technology (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- EE-77-S-02-4267
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- assay; mutagenesis; heterozygous; diploid; human; lymphoblasts; disclosed; determining; mutagenic; damage; caused; administration; suspected; mutagen; lymphoblastoid; cell; lines; locus; employed; thymidine; kinase; uridine; cytidine; deaminase; cells; contain; genes; enzymes; heterozygotes; cell lines; damage caused; cell line; cells contain; diploid human; /435/
Citation Formats
Skopek, Thomas R, Liber, Howard L, Penman, Bruce W, Thilly, William G, and Hoppe, IV, Henry. Assay for mutagenesis in heterozygous diploid human lymphoblasts. United States: N. p., 1981.
Web.
Skopek, Thomas R, Liber, Howard L, Penman, Bruce W, Thilly, William G, & Hoppe, IV, Henry. Assay for mutagenesis in heterozygous diploid human lymphoblasts. United States.
Skopek, Thomas R, Liber, Howard L, Penman, Bruce W, Thilly, William G, and Hoppe, IV, Henry. Thu .
"Assay for mutagenesis in heterozygous diploid human lymphoblasts". United States. https://www.osti.gov/servlets/purl/864055.
@article{osti_864055,
title = {Assay for mutagenesis in heterozygous diploid human lymphoblasts},
author = {Skopek, Thomas R and Liber, Howard L and Penman, Bruce W and Thilly, William G and Hoppe, IV, Henry},
abstractNote = {An assay is disclosed for determining mutagenic damage caused by the administration of a known or suspected mutagen to diploid human lymphoblastoid cell lines. The gene locus employed for this assay is the gene for thymidine kinase, uridine kinase, or cytidine deaminase. Since human lymphoblastoid cells contain two genes for these enzymes, heterozygotes of human lymphoblastoid cells are used in this assay.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1981},
month = {Thu Jan 01 00:00:00 EST 1981}
}
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