Absorption-emission optrode and methods of use thereof
Abstract
A method and apparatus are described for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance. 4 figs.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 7070317
- Patent Number(s):
- 4929561
- Application Number:
- PPN: US 6-763557
- Assignee:
- Univ. of California, Oakland, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 8 Aug 1985
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; FLUIDS; FLUORESCENCE SPECTROSCOPY; SPECTROMETERS; DESIGN; CHEMICAL PROPERTIES; EMISSION SPECTRA; MODULATION; PHYSICAL PROPERTIES; EMISSION SPECTROSCOPY; MEASURING INSTRUMENTS; SPECTRA; SPECTROSCOPY; 400102* - Chemical & Spectral Procedures
Citation Formats
Hirschfeld, T B. Absorption-emission optrode and methods of use thereof. United States: N. p., 1990.
Web.
Hirschfeld, T B. Absorption-emission optrode and methods of use thereof. United States.
Hirschfeld, T B. Tue .
"Absorption-emission optrode and methods of use thereof". United States.
@article{osti_7070317,
title = {Absorption-emission optrode and methods of use thereof},
author = {Hirschfeld, T B},
abstractNote = {A method and apparatus are described for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 29 00:00:00 EDT 1990},
month = {Tue May 29 00:00:00 EDT 1990}
}