Method of saccharifying cellulose
Abstract
A method is disclosed of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of thiol reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 6981250
- Application Number:
- ON: DE84011079
- Assignee:
- Dept. of Energy
- DOE Contract Number:
- AC02-77ET20030
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; CELLULOSE; ENZYMATIC HYDROLYSIS; CALCIUM COMPOUNDS; CELLULASE; CLOSTRIDIUM THERMOCELLUM; COTTON; PH VALUE; THIOLS; ALKALINE EARTH METAL COMPOUNDS; BACTERIA; CARBOHYDRATES; CHEMICAL REACTIONS; CLOSTRIDIUM; DECOMPOSITION; ENZYMES; GLYCOSYL HYDROLASES; HYDROLASES; HYDROLYSIS; LYSIS; MICROORGANISMS; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
Citation Formats
Johnson, E A, Demain, A L, and Madia, A. Method of saccharifying cellulose. United States: N. p., 1983.
Web.
Johnson, E A, Demain, A L, & Madia, A. Method of saccharifying cellulose. United States.
Johnson, E A, Demain, A L, and Madia, A. Fri .
"Method of saccharifying cellulose". United States.
@article{osti_6981250,
title = {Method of saccharifying cellulose},
author = {Johnson, E A and Demain, A L and Madia, A},
abstractNote = {A method is disclosed of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of thiol reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri May 13 00:00:00 EDT 1983},
month = {Fri May 13 00:00:00 EDT 1983}
}
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