Hybrid slab-microchannel gel electrophoresis system
Abstract
A hybrid slab-microchannel gel electrophoresis system is described. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate. 4 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 645954
- Patent Number(s):
- 5746901
- Application Number:
- PAN: 8-628,309
- Assignee:
- Univ. Of California, Oakland, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 5 May 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 40 CHEMISTRY; ELECTROPHORESIS; SEPARATION EQUIPMENT; HYBRID SYSTEMS; DESIGN; GELS
Citation Formats
Balch, J W, Carrano, A V, Davidson, J C, and Koo, J C. Hybrid slab-microchannel gel electrophoresis system. United States: N. p., 1998.
Web.
Balch, J W, Carrano, A V, Davidson, J C, & Koo, J C. Hybrid slab-microchannel gel electrophoresis system. United States.
Balch, J W, Carrano, A V, Davidson, J C, and Koo, J C. Tue .
"Hybrid slab-microchannel gel electrophoresis system". United States.
@article{osti_645954,
title = {Hybrid slab-microchannel gel electrophoresis system},
author = {Balch, J W and Carrano, A V and Davidson, J C and Koo, J C},
abstractNote = {A hybrid slab-microchannel gel electrophoresis system is described. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 05 00:00:00 EDT 1998},
month = {Tue May 05 00:00:00 EDT 1998}
}