Method for performing site-specific affinity fractionation for use in DNA sequencing
Abstract
A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between the cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting the extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to the extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from the array. 14 figs.
- Inventors:
- Issue Date:
- Sponsoring Org.:
- USDOE; USDOE, Washington, DC (United States)
- OSTI Identifier:
- 6281441
- Patent Number(s):
- 5905024
- Application Number:
- PPN: US 8-768893
- Assignee:
- Univ. of Chicago, IL (United States)
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 17 Dec 1996
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; AFFINITY; DNA; DNA HYBRIDIZATION; DNA SEQUENCING; MOLECULAR STRUCTURE; OLIGONUCLEOTIDES; BIOTECHNOLOGY; GENETIC ENGINEERING; HYBRIDIZATION; NUCLEIC ACID HYBRIDIZATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 550200* - Biochemistry
Citation Formats
Mirzabekov, A D, Lysov, Y P, and Dubley, S A. Method for performing site-specific affinity fractionation for use in DNA sequencing. United States: N. p., 1999.
Web.
Mirzabekov, A D, Lysov, Y P, & Dubley, S A. Method for performing site-specific affinity fractionation for use in DNA sequencing. United States.
Mirzabekov, A D, Lysov, Y P, and Dubley, S A. Tue .
"Method for performing site-specific affinity fractionation for use in DNA sequencing". United States.
@article{osti_6281441,
title = {Method for performing site-specific affinity fractionation for use in DNA sequencing},
author = {Mirzabekov, A D and Lysov, Y P and Dubley, S A},
abstractNote = {A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between the cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting the extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to the extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from the array. 14 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 18 00:00:00 EDT 1999},
month = {Tue May 18 00:00:00 EDT 1999}
}