Transposon-containing DNA cloning vector and uses thereof
Abstract
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Washington, Seattle, WA (United States); Univ. of Connecticut, Storrs, CT (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 512421
- Patent Number(s):
- 5645991
- Application Number:
- PAN: 8-403,582
- Assignee:
- Univ. of Connecticut, Storrs, CT (United States); Washington Univ., St. Louis, MO (United States)
- DOE Contract Number:
- FG02-89ER60862; FG02-90ER61000
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 8 Jul 1997
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; PLASMIDS; GENETIC ENGINEERING; DNA SEQUENCING; TRANSPOSONS
Citation Formats
Berg, C M, Berg, D E, and Wang, G. Transposon-containing DNA cloning vector and uses thereof. United States: N. p., 1997.
Web.
Berg, C M, Berg, D E, & Wang, G. Transposon-containing DNA cloning vector and uses thereof. United States.
Berg, C M, Berg, D E, and Wang, G. Tue .
"Transposon-containing DNA cloning vector and uses thereof". United States.
@article{osti_512421,
title = {Transposon-containing DNA cloning vector and uses thereof},
author = {Berg, C M and Berg, D E and Wang, G},
abstractNote = {The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 08 00:00:00 EDT 1997},
month = {Tue Jul 08 00:00:00 EDT 1997}
}
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