Electronic method for autofluorography of macromolecules on two-D matrices. [Patent application]
Abstract
A method for detecting, localizing, and quantifying macromolecules contained in a two-dimensional matrix is provided which employs a television-based position sensitive detection system. A molecule-containing matrix may be produced by conventional means to produce spots of light at the molecule locations which are detected by the television system. The matrix, such as a gel matrix, is exposed to an electronic camera system including an image-intensifier and secondary electron conduction camera capable of light integrating times of many minutes. A light image stored in the form of a charge image on the camera tube target is scanned by conventional television techniques, digitized, and stored in a digital memory. Intensity of any point on the image may be determined from the number at the memory address of the point. The entire image may be displayed on a television monitor for inspection and photographing or individual spots may be analyzed through selected readout of the memory locations. Compared to conventional film exposure methods, the exposure time may be reduced 100 to 1000 times.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 5084824
- Application Number:
- ON: DE82020861
- Assignee:
- Dept. of Energy
- DOE Contract Number:
- W-7405-ENG-26
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; FLUOROSCOPY; IMAGE PROCESSING; MOLECULES; CAMERAS; ELECTRON BEAMS; GELS; IMAGE INTENSIFIERS; MATRIX ISOLATION; POSITION SENSITIVE DETECTORS; TELEVISION CAMERAS; VISIBLE RADIATION; BEAMS; BIOMEDICAL RADIOGRAPHY; COLLOIDS; DIAGNOSTIC TECHNIQUES; DISPERSIONS; ELECTROMAGNETIC RADIATION; LEPTON BEAMS; MEASURING INSTRUMENTS; MEDICINE; NUCLEAR MEDICINE; PARTICLE BEAMS; PROCESSING; RADIATION DETECTORS; RADIATIONS; RADIOLOGY; 550200* - Biochemistry; 440300 - Miscellaneous Instruments- (-1989)
Citation Formats
Davidson, J B, and Case, A L. Electronic method for autofluorography of macromolecules on two-D matrices. [Patent application]. United States: N. p., 1981.
Web.
Davidson, J B, & Case, A L. Electronic method for autofluorography of macromolecules on two-D matrices. [Patent application]. United States.
Davidson, J B, and Case, A L. Wed .
"Electronic method for autofluorography of macromolecules on two-D matrices. [Patent application]". United States.
@article{osti_5084824,
title = {Electronic method for autofluorography of macromolecules on two-D matrices. [Patent application]},
author = {Davidson, J B and Case, A L},
abstractNote = {A method for detecting, localizing, and quantifying macromolecules contained in a two-dimensional matrix is provided which employs a television-based position sensitive detection system. A molecule-containing matrix may be produced by conventional means to produce spots of light at the molecule locations which are detected by the television system. The matrix, such as a gel matrix, is exposed to an electronic camera system including an image-intensifier and secondary electron conduction camera capable of light integrating times of many minutes. A light image stored in the form of a charge image on the camera tube target is scanned by conventional television techniques, digitized, and stored in a digital memory. Intensity of any point on the image may be determined from the number at the memory address of the point. The entire image may be displayed on a television monitor for inspection and photographing or individual spots may be analyzed through selected readout of the memory locations. Compared to conventional film exposure methods, the exposure time may be reduced 100 to 1000 times.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Dec 30 00:00:00 EST 1981},
month = {Wed Dec 30 00:00:00 EST 1981}
}