Rapid purification of circular DNA by triplex-mediated affinity capture
Abstract
A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide probe. The oligonucleotide is bound to an immobilizing support which facilitates the immobilization and purification of target DNA molecules. Non-target DNA molecules and other contaminating cellular material are easily removed by washing. The triple-helical structure is destabilized by raising the pH, leaving purified target DNA in the supernatant and reusable affinity capture oligonucleotide secured to the immobilizing support. 3 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- OSTI Identifier:
- 415738
- Patent Number(s):
- 5591841
- Application Number:
- PAN: 8-317,102
- Assignee:
- PTO; SCA: 550200; PA: EDB-97:016814; SN: 97001714576
- DOE Contract Number:
- FG02-90ER61026
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 7 Jan 1997
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA; PURIFICATION; MOLECULAR STRUCTURE; MOLECULAR BIOLOGY; HELICAL CONFIGURATION
Citation Formats
Ji, H, and Smith, L M. Rapid purification of circular DNA by triplex-mediated affinity capture. United States: N. p., 1997.
Web.
Ji, H, & Smith, L M. Rapid purification of circular DNA by triplex-mediated affinity capture. United States.
Ji, H, and Smith, L M. Tue .
"Rapid purification of circular DNA by triplex-mediated affinity capture". United States.
@article{osti_415738,
title = {Rapid purification of circular DNA by triplex-mediated affinity capture},
author = {Ji, H and Smith, L M},
abstractNote = {A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide probe. The oligonucleotide is bound to an immobilizing support which facilitates the immobilization and purification of target DNA molecules. Non-target DNA molecules and other contaminating cellular material are easily removed by washing. The triple-helical structure is destabilized by raising the pH, leaving purified target DNA in the supernatant and reusable affinity capture oligonucleotide secured to the immobilizing support. 3 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 07 00:00:00 EST 1997},
month = {Tue Jan 07 00:00:00 EST 1997}
}
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