DNA sequencing with pyrophosphatase
Abstract
A kit or solution is disclosed for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule and having a second single-stranded region homologous to the first single-stranded region. The first agent is able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture. The second agent is able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.
- Inventors:
- Issue Date:
- Research Org.:
- Harvard University
- OSTI Identifier:
- 203883
- Patent Number(s):
- 5498523
- Application Number:
- PAN: 8-275,339; CNN: Grant A1-06045
- Assignee:
- Harvard Coll., Cambridge, MA (United States)
- DOE Contract Number:
- FG02-88ER60688
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 12 Mar 1996
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; PHOSPHATASES; DNA SEQUENCING; DNA SEQUENCERS; DESIGN; MOLECULAR BIOLOGY; OLIGONUCLEOTIDES; MOLECULAR STRUCTURE
Citation Formats
Tabor, S, and Richardson, C C. DNA sequencing with pyrophosphatase. United States: N. p., 1996.
Web.
Tabor, S, & Richardson, C C. DNA sequencing with pyrophosphatase. United States.
Tabor, S, and Richardson, C C. Tue .
"DNA sequencing with pyrophosphatase". United States.
@article{osti_203883,
title = {DNA sequencing with pyrophosphatase},
author = {Tabor, S and Richardson, C C},
abstractNote = {A kit or solution is disclosed for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule and having a second single-stranded region homologous to the first single-stranded region. The first agent is able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture. The second agent is able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 12 00:00:00 EST 1996},
month = {Tue Mar 12 00:00:00 EST 1996}
}
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