Title: Promoter useful for high expression of a heterologous gene of interest in Aspergillus niger

Abstract

The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.

Inventors:

Gladden, John M.; Campen, Saori Amaike; Zhang, Jinxiang; Magnuson, Jon K.; Baker, Scott E.; Simmons, Blake A.

Issue Date:

Tue Aug 16 00:00:00 EDT 2022

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1924985

Patent Number(s):

11414653

Application Number:

16/124,127

Assignee:

National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM); Battelle Memorial Institute (Richland, WA); The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/38 - from Aspergillus

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/80 - for fungi
C12N9/242 - {Fungal source}
C12N9/2445 - {Beta-glucosidase

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y302/0102 - Alpha-glucosidase
C12Y302/01021 - Beta-glucosidase

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DOE Contract Number:  

AC02-05CH11231

Resource Type:

Patent

Resource Relation:

Patent File Date: 09/06/2018

Country of Publication:

United States

Language:

English