Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes
Abstract
Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- AGRIVIDA, INC., Medford, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1460546
- Patent Number(s):
- 10006038
- Application Number:
- 14/961,426
- Assignee:
- AGRIVIDA, INC. (Medford, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AR0000042
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2015 Dec 07
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Raab, R. Michael, Zhang, Dongcheng, and Bougri, Oleg. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes. United States: N. p., 2018.
Web.
Raab, R. Michael, Zhang, Dongcheng, & Bougri, Oleg. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes. United States.
Raab, R. Michael, Zhang, Dongcheng, and Bougri, Oleg. Tue .
"Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes". United States. https://www.osti.gov/servlets/purl/1460546.
@article{osti_1460546,
title = {Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes},
author = {Raab, R. Michael and Zhang, Dongcheng and Bougri, Oleg},
abstractNote = {Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 26 00:00:00 EDT 2018},
month = {Tue Jun 26 00:00:00 EDT 2018}
}
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Works referenced in this record:
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
journal, June 1997
- Chong, Shaorong; Mersha, Fana B.; Comb, Donald G.
- Gene, Vol. 192, Issue 2, p. 271-281
Global and grain-specific accumulation of glycoside hydrolase family 10 xylanases in transgenic maize (Zea mays): Maize xylanase expression
journal, June 2011
- Gray, Benjamin N.; Bougri, Oleg; Carlson, Alvar R.
- Plant Biotechnology Journal, Vol. 9, Issue 9
Self-processing plants and plant parts
patent, September 2006
- Lanahan, Michael B.; Basu, Shib Sankar; Batie, Christopher J.
- US Patent Document 7,102,057
Method for generating split, non-transferable genes that are able to express an active protein product
patent, February 2005
- Xu, Ming-Qun; Evans, Thomas C.; Pradhan, Sriharsa
- US Patent Document 6,858,775
Ribonucleotide reductase in the archaeon Pyrococcus furiosus: A critical enzyme in the evolution of DNA genomes?
journal, January 1997
- Riera, J.; Robb, F. T.; Weiss, R.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 2, p. 475-478
Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol
journal, June 2008
- Sticklen, Mariam B.
- Nature Reviews Genetics, Vol. 9, Issue 6
Sulfite Pretreatment For Biorefining Biomass
patent-application, December 2009
- Wang, Gaosheng; Pan, Xuejun; Zhu, Jun Yong
- US Patent Application 12/425773; 20090298149
Nucleic acid sequences encoding cell wall-degrading enzymes and use to engineer resistance to fusarium and other pathogens
patent-application, July 2003
- Okubara, Patricia A.; Blechl, Ann F.; Hohn, Thomas M.
- US Patent Application 10/316754; 20030131376
The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts
journal, January 1985
- Schreier, Peter H.; Seftor, Elisabeth A.; Schell, Jozef
- The EMBO Journal, Vol. 4, Issue 1, p. 25-32
Commercial production of laccase in plants
patent, October 2004
- Howard, John; Hood, Elizabeth; Jilka, Joseph
- US Patent Document 6,800,792
Methods for Determining Cellulolytic Enhancing Activity of a Polypeptide
patent-application, June 2010
- Sweeney, Matthew David; Vlasenko, Elena
- US Patent Application 12/641075; 20100159494
Transgenic Plants Expressing CIVPS or Intein Modified Proteins and Related Method
patent-application, November 2013
- Raab, R. Michael
- US Patent Application 13/962520; 20130318655
Semisynthesis of cytotoxic proteins using a modified protein splicing element
journal, November 1998
- Evans, Thomas C.; Benner, Jack; Xu, Ming-Qun
- Protein Science, Vol. 7, Issue 11
The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea
journal, May 1997
- Genschik, P.
- The EMBO Journal, Vol. 16, Issue 10
Transgenic cover plants containing hemicellulase and cellulase which degrade lignin and cellulose to fermentable sugars
patent, January 2012
- Sticklen, Masomeh B.
- US Patent Document 8,093,456
Stable expression of a thermostable xylanase of Clostridium thermocellum in cultured tobacco cells
journal, January 2003
- Kimura, Tetsuya; Mizutani, Tomomi; Sakka, Kazuo
- Journal of Bioscience and Bioengineering, Vol. 95, Issue 4, p. 397-400
Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation.
journal, December 1994
- Xu, M. Q.; Comb, D. G.; Paulus, H.
- The EMBO Journal, Vol. 13, Issue 23
Transgenic plants expressing a cellulase
patent, July 2013
- Lebel, Edouard; Heifetz, Peter; Ward, Eric R.
- US Patent Document 8,481,810
The Thy Pol-2 intein of Thermococcus hydrothermalis is an isoschizomer of PI-TliI and PI-TfuII endonucleases
journal, November 2000
- Saves, Isabelle; Eleaume, Heïdy; Dietrich, Jacques
- Nucleic Acids Research, Vol. 28, Issue 21, p. 4391-4396
Expression of microbial proteins in plants for production of plants with improved properties
patent-application, December 2003
- Cao, Yongwei; Hinkle, Gregory J.; Slater, Steven C.
- US Patent Application 10/369493; 20030233675
Modified proteins comprising controllable intervening protein sequences or their elements methods of producing same and methods for purification of a target protein comprised by a modified protein
patent, November 1998
- Comb, Donald G.; Perler, Francine B.; Jack, William E.
- US Patent Document 5,834,247
Rice nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
patent-application, June 2004
- La Rosa, Thomas; Kovalic, David; Zhou, Yihua
- US Patent Document 10/437963; 20040123343
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962
- Murashige, Toshio; Skoog, Folke
- Physiologia Plantarum, Vol. 15, Issue 3, p. 473-497
Dual targeting of xylanase to chloroplasts and peroxisomes as a means to increase protein accumulation in plant cells
journal, November 2005
- Hyunjong, Bae; Lee, Dae-Seok; Hwang, Inhwan
- Journal of Experimental Botany, Vol. 57, Issue 1
The in Vitro Ligation of Bacterially Expressed Proteins Using an Intein from Methanobacterium thermoautotrophicum
journal, February 1999
- Evans, Thomas C.; Benner, Jack; Xu, Ming-Qun
- Journal of Biological Chemistry, Vol. 274, Issue 7
Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules.
journal, January 1987
- Tingey, S. V.; Walker, E. L.; Coruzzi, G. M.
- The EMBO Journal, Vol. 6, Issue 1, p. 1-9
Xylanases, Nucleic Acids Encoding Them and Methods for Making and Using Them
patent-application, June 2009
- Weiner, David; Blum, David; Varvak, Alexander
- US Patent Application 12/279326; 20090155238
Encryption of traits using split gene sequences and engineered genetic elements
patent, March 2003
- Patten, Phillip A.; Lassner, Michael
- US Patent Document 6,531,316
Consolidated Pretreatment and Hydrolysis of Plant Biomass Expressing Cell Wall Degrading Enzymes
patent-application, October 2012
- Raab, R. Michael; Zhang, Dongcheng; Bougri, Oleg
- US Patent Application 13/414627; 20120258503
Genomic sequence of a 12S seed storage protein from oilseed rape ( Brassica napus c.v. jet neuf)
journal, January 1989
- Ryan, Anderson J.; Royal, Christopher L.; Hutchinson, Joe
- Nucleic Acids Research, Vol. 17, Issue 9, p. 3584-3584
Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
patent-application, September 2002
- Sticklen, Masomeh B.; Dale, Bruce F.; Maqbool, Shahina B.
- US Patent Application 09/981900; 20020138878
Corn as a production system for human and animal vaccines
journal, January 2003
- Streatfield, S.
- Vaccine, Vol. 21, Issue 7-8
Glucoamylase variants
patent, January 2012
- Nielsen, Bjarne Roenfeldt; Svendsen, Allan; Pedersen, Henrik
- US Patent Document 8,101,392
Invariant Asp-1122 and Asp-1124 Are Essential Residues for Polymerization Catalysis of Family D DNA Polymerase from Pyrococcus horikoshii
journal, April 2001
- Shen, Yulong; Musti, Krishnasastry; Hiramoto, Madoka
- Journal of Biological Chemistry, Vol. 276, Issue 29, p. 27376-27383
Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
patent, January 2012
- Gray, Kevin A.; Zhao, Lishan; Cayouette, Michelle
- US Patent Document 8,101,393
Gene transfer to plants by diverse species of bacteria
journal, February 2005
- Broothaerts, Wim; Mitchell, Heidi J.; Weir, Brian
- Nature, Vol. 433, Issue 7026
Self-processing plants and plant parts
patent, April 2011
- Lanahan, Michael B.; Basu, Shib Sankar; Batie, Christopher J.
- US Patent Document 7,919,681
Plants with reduced activity of a starch phosphorylating enzyme
patent, April 2011
- Frohberg, Claus; Koetting, Oliver; Ritte, Gerhard
- US Patent Document 7,919,682
Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010
- Alvira, P.; Tomás-Pejó, E.; Ballesteros, M.
- Bioresource Technology, Vol. 101, Issue 13, p. 4851-4861
Improvement of ethanol production from starch by recombinant yeast through manipulation of environmental factors
journal, October 2002
- Mete Altıntaş, M.; Ülgen, Kutlu Ö; Kırdar, Betül
- Enzyme and Microbial Technology, Vol. 31, Issue 5
Improving Enzymes for Biomass Conversion: A Basic Research Perspective
journal, January 2010
- Banerjee, Goutami; Scott-Craig, John S.; Walton, Jonathan D.
- BioEnergy Research, Vol. 3, Issue 1
Purification of Proteins Fused to Either the Amino or Carboxy Terminus of the Mycobacterium xenopi Gyrase A Intein
journal, July 1999
- Southworth, Maurice W.; Amaya, Kensey; Evans, Thomas C.
- BioTechniques, Vol. 27, Issue 1, p. 110-120
Production of a recombinant xylanase in plants and its potential for pulp biobleaching applications
journal, June 2008
- Bae, Hyeun-Jong; Kim, Hyun Joo; Kim, Yoon Soo
- Bioresource Technology, Vol. 99, Issue 9, p. 3513-3519
Expression of xylanase with high specific activity from Streptomyces olivaceoviridis A1 in transgenic potato plants (Solanum tuberosum L.)
journal, January 2007
- Yang, Peilong; Wang, Yaru; Bai, Yingguo
- Biotechnology Letters, Vol. 29, Issue 4
Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
journal, July 1990
- Gordon-Kamm, W. J.; Spencer, T. M.; Mangano, M. L.
- The Plant Cell
Compilation and analysis of intein sequences
journal, March 1997
- Perler, Francine B.; Olsen, Gary J.; Adam, Eric
- Nucleic Acids Research, Vol. 25, Issue 6, p. 1087-1093
Cloning, expression, and characterization of DNA polymerase I from the hyperthermophilic archaea Thermococcus fumicolans
journal, August 2000
- Cambon-Bonavita, Marie-Anne; Schmitt, Philippe; Zieger, Montserrat
- Extremophiles, Vol. 4, Issue 4
High Level Expression and Accumulation of Heterologous Polypeptide in Plant Tissue
patent-application, July 2009
- Miles, Stacy
- US Patent Document 12/359421; 20090193541
Overexpression of human erythropoietin (EPO) affects plant morphologies: retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis
journal, December 2004
- Cheon, Ban Yoon; Kim, Hae Jin; Oh, Kyung Hee
- Transgenic Research, Vol. 13, Issue 6
Self processing plants and plant parts
patent, July 2009
- Lanahan, Michael B.; Basu, Shib Sankar; Batie, Christopher J.
- US Patent Document 7,557,262
Metazoan cellulase genes from termites: intron/exon structures and sites of expression
journal, October 1999
- Tokuda, Gaku; Lo, Nathan; Watanabe, Hirofumi
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 1447, Issue 2-3
Expression Enhancement of a Rice Polyubiquitin Gene Promoter
journal, January 2006
- Sivamani, Elumalai; Qu, Rongda
- Plant Molecular Biology, Vol. 60, Issue 2, p. 225-239
Microwave-assisted pretreatment of woody biomass with ammonium molybdate activated by H2O2
journal, February 2011
- Verma, Pradeep; Watanabe, Takahito; Honda, Yoichi
- Bioresource Technology, Vol. 102, Issue 4, p. 3941-3945
A genetic system yields self-cleaving inteins for bioseparations
journal, September 1999
- Wood, David W.; Wu, Wei; Belfort, Georges
- Nature Biotechnology, Vol. 17, Issue 9
Transgenic Plants Expressing Intein Modified Proteins and Associated Processes for Bio-Pharmaceutical Production
patent-application, May 2008
- Raab, R. Michael; Jensen, Kyle L.; Ruping, Karl
- US Patent Application 11/596821; 20080115243
Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
patent-application, August 2006
- Sticklen, Masomeh B.; Dale, Bruce E.; Maqbool, Shahina B.
- US Patent Application 11/399675; 20060179513
In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
journal, January 2011
- Brunecky, Roman; Selig, Michael J.; Vinzant, Todd B.
- Biotechnology for Biofuels, Vol. 4, Issue 1
Complete nucleotide sequence of alfalfa mosaic virus RNA 4
journal, January 1980
- Brederode, Frans Th.; Koper-Zwarthoff, Ellen C.; Bol, John F.
- Nucleic Acids Research, Vol. 8, Issue 10
Nucleic acid molecules from maize and their use for the production of modified starch
patent, March 2007
- Kossmann, Jens; Emmermann, Michael
- US Patent Document 7,186,898
Breaking up is hard to do
journal, January 1998
- Stoddard, Barry L.; Pietrokovski, Shmuel
- Nature Structural Biology, Vol. 5, Issue 1
Transgenic Plants Expressing CIVPS or Intein Modified Proteins and Related Method
patent-application, June 2005
- Raab, R. Michael
- US Patent Application 10/886393; 20050125860
Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor
journal, December 2000
- Wood, D. W.; Derbyshire, V.; Wu, W.
- Biotechnology Progress, Vol. 16, Issue 6
NMR Observation of Selected Segments in a Larger Protein: Central-Segment Isotope Labeling through Intein-Mediated Ligation
journal, December 1999
- Otomo, Takanori; Ito, Nobutoshi; Kyogoku, Yoshimasa
- Biochemistry, Vol. 38, Issue 49, p. 16040-16044
Expression of phytase in plants
patent, February 2000
- Van Ooijen, Albert Johannes Joseph; Rietveld, Krun; Hoekema, Andreas
- US Patent Document 6,022,846
Production of beta-glucosidase, hemicellulase and ligninase in E1 and FLC-cellulase-transgenic plants
patent-application, August 2007
- Sticklen, Masomeh
- US Patent Application 11/489234; 20070192900
Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein
journal, April 1998
- Chong, Shaorong; Williams, Kay S.; Wotkowicz, Chad
- Journal of Biological Chemistry, Vol. 273, Issue 17
Genome sequence of Halobacterium species NRC-1
journal, October 2000
- Ng, W. V.; Kennedy, S. P.; Mahairas, G. G.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 22, p. 12176-12181
Protein Splicing: Characterization of the Aminosuccinimide Residue at the Carboxyl Terminus of the Excised Intervening Sequence
journal, August 1995
- Shao, Yang; Xu, Ming-Qun; Paulus, Henry
- Biochemistry, Vol. 34, Issue 34, p. 10844-10850
An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile
journal, September 2000
- Southworth, M. W.
- The EMBO Journal, Vol. 19, Issue 18
Dry-Grind Process for Fuel Ethanol by Continuous Fermentation and Stripping
journal, August 2000
- Taylor, F.; Kurantz, M. J.; Goldberg, N.
- Biotechnology Progress, Vol. 16, Issue 4
Fertile transgenic corn plants
patent, December 2000
- Lundquist, Ronald C.; Walters, David A.; Kirihara, Julie A.
- US Patent Document 6,160,208
High-yield production of a human therapeutic protein in tobacco chloroplasts
journal, March 2000
- Staub, Jeffrey M.; Garcia, Bradley; Graves, Julie
- Nature Biotechnology, Vol. 18, Issue 3
Coordinated development of leading biomass pretreatment technologies
journal, December 2005
- Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T.
- Bioresource Technology, Vol. 96, Issue 18, p. 1959-1966
Transgenic plants expressing CIVPS or intein modified proteins and related method
patent, March 2014
- Raab, R. Michael
- US Patent Document 8,664,476
A short domain of the plant vacuolar protein phytohemagglutinin targets invertase to the yeast vacuole.
journal, June 1990
- Tague, B. W.; Dickinson, C. D.; Chrispeels, M. J.
- The Plant Cell, Vol. 2, Issue 6
The curious case of protein splicing: Mechanistic insights suggested by protein semisynthesis
journal, May 1993
- Wallace, Carmichael J. A.
- Protein Science, Vol. 2, Issue 5
Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers
journal, July 1996
- Komari, Toshihiko; Hiei, Yukoh; Saito, Yasuhito
- The Plant Journal, Vol. 10, Issue 1
Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain
journal, January 2001
- Chen, Lixin; Pradhan, Sriharsa; Evans, Thomas C.
- Gene, Vol. 263, Issue 1-2, p. 39-48
The intein of the Thermoplasma A-ATPase A subunit: Structure, evolution and expression in E. coli
journal, January 2001
- Senejani, Alireza G.; Hilario, Elena; Gogarten, J. Peter
- BMC Biochemistry, Vol. 2, Article No. 13
Inteins of Thermococcus fumicolans DNA Polymerase Are Endonucleases with Distinct Enzymatic Behaviors
journal, January 2000
- Saves, Isabelle; Ozanne, Valérie; Dietrich, Jacques
- Journal of Biological Chemistry, Vol. 275, Issue 4, p. 2335-2341
Methods for Increasing Starch Content in Plants
patent-application, May 2009
- Lanahan, Michael B.; Steffens, John
- US Patent Application 12/264620; 20090119800
Fertile transgenic rice plants regenerated from transformed protoplasts
journal, March 1989
- Shimamoto, Ko; Terada, Rie; Izawa, Takeshi
- Nature, Vol. 338, Issue 6212, p. 274-276
Analysis of a chimeric class-I patatin-GUS gene in transgenic potato plants: High-level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants
journal, January 1989
- Wenzler, Herman C.; Mignery, Gregory A.; Fisher, Linda M.
- Plant Molecular Biology, Vol. 12, Issue 1, p. 41-50
Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes
patent, February 2016
- Raab, R. Michael; Zhang, Dongcheng; Bougri, Oleg
- US Patent Document 9,249,474
Delignification of Biomass with Sequential Base Treatment
patent-application, May 2010
- Sabesan, Subramaniam; Spado, Christina Jacy
- US Patent Application 12/621599; 20100124771
Lightning strikes twice: Intron-intein coincidence
journal, February 1998
- Derbyshire, V.; Belfort, M.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 4
The complete genome of the hyperthermophilic bacterium Aquifex aeolicus
journal, March 1998
- Deckert, Gerard; Warren, Patrick V.; Gaasterland, Terry
- Nature, Vol. 392, Issue 6674
Heterologous Biomass Degrading Enzyme Expression in Thermoanaerobacterium Saccharolyticum
patent-application, February 2012
- Hau, Heidi; Rice, Chales; Herring, Chris
- US Patent Application 13/141952; 20120040409
Enhanced turnover of transitory starch by expression of up-regulated ADP-glucose pyrophosphorylases in Arabidopsis thaliana
journal, January 2006
- Obana, Yumiko; Omoto, Daisuke; Kato, Chikako
- Plant Science, Vol. 170, Issue 1, p. 1-11
Intein-mediated protein splicing
patent-application, September 2003
- Yadav, Narendra S.; Yang, Jianjun
- US Patent Application 10/356088; 20030167533
Methods for Enzymatic Hydrolysis of Lignoclellulose
patent-application, September 2007
- Duck, Nicholas B.; Carr, Brian; Koziel, Michael G.
- US Patent Application 11/748727; 20070218530
Fertile transgenic corn plants
patent, July 1998
- Lundquist, Ronald C.; Walters, David A.
- US Patent Document 5,780,708
Synthesis, processing and export of cytoplasmic endo‐β‐1,4‐xylanase from barley aleurone during germination
journal, April 2001
- Caspers, Martien P. M.; Lok, Finn; Sinjorgo, Karin M. C.
- The Plant Journal, Vol. 26, Issue 2, p. 191-204
Ionic liquid pretreatment of cellulosic biomass: Enzymatic hydrolysis and ionic liquid recycle
journal, December 2010
- Shill, Kierston; Padmanabhan, Sasisanker; Xin, Qin
- Biotechnology and Bioengineering, Vol. 108, Issue 3, p. 511-520
Plant cell wall reconstruction toward improved lignocellulosic production and processability
journal, February 2010
- Abramson, Miron; Shoseyov, Oded; Shani, Ziv
- Plant Science, Vol. 178, Issue 2, p. 61-72
InBase: the Intein Database
journal, January 2002
- Perler, F. B.
- Nucleic Acids Research, Vol. 30, Issue 1, p. 383-384
Transformation of Poplar by Agrobacterium tumefaciens
journal, June 1986
- Parsons, Thomas J.; Sinkar, Vilas P.; Stettler, Reinhard F.
- Nature Biotechnology, Vol. 4, Issue 6, p. 533-536
Statistical Modeling, Phylogenetic Analysis and Structure Prediction of a Protein Splicing Domain Common to Inteins and Hedgehog Proteins
journal, January 1997
- Dalgaard, Jacob Z.; Moser, Michael J.; Hughey, Richard
- Journal of Computational Biology, Vol. 4, Issue 2
Transgenic plants expressing a cellulase
patent, April 2008
- Lebel, Edouard; Heifetz, Peter; Ward, Eric R.
- US Patent Document 7,361,806
Production of Therapeutic Proteins in Transgenic Cereal Crops
patent-application, August 2003
- Tackaberry, Eilleen; Dudani, Anil; Ganz, Peter
- US Patent Application 10/230365; 20030159182
Characterization of two intein homing endonucleases encoded in the DNA polymerase gene of Pyrococcus kodakaraensis strain KOD1
journal, October 1998
- Nishioka, Motomu; Fujiwara, Shinsuke; Takagi, Masahiro
- Nucleic Acids Research, Vol. 26, Issue 19, p. 4409-4412
Enhancement of growth and cellulose accumulation by overexpression of xyloglucanase in poplar
journal, March 2004
- Park, Yong Woo; Baba, Kei'ichi; Furuta, Yuzo
- FEBS Letters, Vol. 564, Issue 1-2, p. 183-187
Green chemistry yields a better plastic
journal, October 1999
- Poirier, Yves
- Nature Biotechnology, Vol. 17, Issue 10, p. 960-961
Double Stranded RNA Structures and Constructs, and Methods for Generating and Using the Same
patent-application, October 2009
- Pachuk, Catherine J.; Satishchandran, C.; Chopra, Maninder
- US Patent Application 12/246258; 20090258930
The tomato polygalacturonase gene and ripening-specific expression in transgenic plants
journal, January 1988
- Bird, C. R.; Smith, C. J. S.; Ray, J. A.
- Plant Molecular Biology, Vol. 11, Issue 5
Transgenic plants expressing CIVPS or intein modified proteins and related method
patent, August 2012
- Raab, R. Michael
- US Patent Document 8,247,647
Cellulose Digestion in the Wood-Eating Higher Termite, Nasutitermes takasagoensis (Shiraki): Distribution of Cellulases and Properties of Endo-β-1,4-gIucanase
journal, February 1997
- Tokuda, Gaku; Watanabe, Hirofumi; Matsumoto, Tadao
- Zoological Science, Vol. 14, Issue 1
Transgenic plants expressing a cellulase
patent, November 2010
- Lebel, Edouard; Heifetz, Peter; Ward, Eric R.
- US Patent Document 7,838,732
Secretion of a heat-stable fungalβ-glucanase from transgenic, suspension-cultured barley cells
journal, March 1995
- Aspegren, Kristian; Mannonen, Leena; Ritala, Anneli
- Molecular Breeding, Vol. 1, Issue 1
A topA intein in Pyrococcus furiosus and its relatedness to the r-gyr intein of Methanococcus jannaschii
journal, March 1998
- Chute, Ian C.; Hu, Zhuma; Liu, Xiang-Qin
- Gene, Vol. 210, Issue 1, p. 85-92
Protein Splicing in the Absence of an Intein Penultimate Histidine
journal, April 2000
- Chen, Lixin; Benner, Jack; Perler, Francine B.
- Journal of Biological Chemistry, Vol. 275, Issue 27
Organic Solvent Pretreatment of Biomass to Enhance Enzymatic Saccharification
patent-application, June 2010
- Diner, Bruce A.; Fagan, Paul Joseph; Fan, Janine
- US Patent Application 12/639050; 20100159519
Protein trans-Splicing To Produce Herbicide-Resistant Acetolactate Synthase
journal, March 2001
- Sun, L.; Ghosh, I.; Paulus, H.
- Applied and Environmental Microbiology, Vol. 67, Issue 3
Genetic System and Self-Cleaving Inteins Derived Therefrom, Bioseparations and Protein Purification Employing Same, and Methods for Determining Critical, Generalizable Amino acid Residues for Varying Intein Activity
patent, August 2005
- Belfort, Marlene; Belfort, Georges; Derbyshire, Vicky
- US Patent Document 6,933,362
Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase
journal, January 1985
- Van den Broeck, Guido; Timko, Michael P.; Kausch, Albert P.
- Nature, Vol. 313, Issue 6001
Transgenic plants expressing CIVPS or intein modified proteins and related method
patent, May 2010
- Raab, R. Michael
- US Patent Document 7,709,697
Biological pretreatment of wheat straw by Phanerochaete chrysosporium supplemented with inorganic salts
journal, February 2011
- Zeng, Jijiao; Singh, Deepak; Chen, Shulin
- Bioresource Technology, Vol. 102, Issue 3, p. 3206-3214
Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product.
journal, January 1991
- Davis, E. O.; Sedgwick, S. G.; Colston, M. J.
- Journal of Bacteriology, Vol. 173, Issue 18
Pretreatment of switchgrass for sugar production with the combination of sodium hydroxide and lime
journal, February 2011
- Xu, Jiele; Cheng, Jay J.
- Bioresource Technology, Vol. 102, Issue 4, p. 3861-3868
Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005
- Mosier, Nathan; Wyman, Charles; Dale, Bruce
- Bioresource Technology, Vol. 96, Issue 6, p. 673-686
Protein splicing in the maturation of M. tuberculosis RecA protein: A mechanism for tolerating a novel class of intervening sequence
journal, October 1992
- Davis, Elaine O.; Jenner, Peter J.; Brooks, Patricia C.
- Cell, Vol. 71, Issue 2, p. 201-210
Commercial cellulosic ethanol: The role of plant-expressed enzymes
journal, May 2009
- Sainz, Manuel B.
- In Vitro Cellular & Developmental Biology - Plant, Vol. 45, Issue 3, p. 314-329
Method of Preparing More Digestible Animal Feed
patent-application, September 2008
- Abbas, Charles; Bao, Wuli; Beery, Kyle
- US Patent Application 12/042452; 20080220125
Transgenic barley expressing a fungal xylanase gene in the endosperm of the developing grains
journal, January 2000
- Patel, Minesh; Johnson, Jennifer S.; Brettell, Richard I. S.
- Molecular Breeding, Vol. 6, Issue 1, p. 113-124
Biobased Industrial Products: Bioprocess Engineering When Cost Really Counts
journal, October 1999
- Dale, B. E.
- Biotechnology Progress, Vol. 15, Issue 5
Dramatic effects of truncation and sub-cellular targeting on the accumulation of recombinant microbial cellulase in tobacco
journal, September 2001
- Ziegelhoffer, Thomas; Raasch, John A.; Austin-Phillips, Sandra
- Molecular Breeding, Vol. 8, Issue 2, p. 147-158
The starch-related R1 protein is an α-glucan, water dikinase
journal, May 2002
- Ritte, G.; Lloyd, J. R.; Eckermann, N.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 10, p. 7166-7171
Tissue-specific and light-regulated expression of a pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase.
journal, August 1984
- Coruzzi, G.; Broglie, R.; Edwards, C.
- The EMBO Journal, Vol. 3, Issue 8
A review of the production of ethanol from softwood
journal, July 2002
- Galbe, M.; Zacchi, G.
- Applied Microbiology and Biotechnology, Vol. 59, Issue 6
Protein splicing elements: inteins and exteins — a definition of terms and recommended nomenclature
journal, January 1994
- Perler, Francine B.; Davis, Elaine O.; Dean, Gary E.
- Nucleic Acids Research, Vol. 22, Issue 7, p. 1125-1127
Transgenic Plants Expressing CIVPS or Intein Modified Proteins and Related Method
patent-application, June 2010
- Raab, R. Michael
- US Patent Application 12/696800; 20100159510
Identification of an Unusual Intein in Chloroplast ClpP Protease of Chlamydomonas eugametos
journal, May 1997
- Wang, Shenglong; Liu, Xiang-Qin
- Journal of Biological Chemistry, Vol. 272, Issue 18
Multi-Gene Expression Constructs Containing Modified Inteins
patent-application, December 2005
- Snell, Kristi D.
- US Patent Application 11/147546; 20050283850
The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein.
journal, January 1997
- Telenti, A.; Southworth, M.; Alcaide, F.
- Journal of bacteriology, Vol. 179, Issue 20
Metabolic Engineering of Propanediol Pathways
journal, February 1998
- Cameron, D. C.; Altaras, N. E.; Hoffman, M. L.
- Biotechnology Progress, Vol. 14, Issue 1
Energy crops for improved biofuel feedstocks
patent-application, October 2007
- Blaylock, Michael; Ferguson, Bruce; Lee, David
- US Patent Application 11/712593; 20070250961
Fertile transgenic corn plants
patent, January 2000
- Lundquist, Ronald C.; Walters, David A.
- US Patent Document 6,013,863
Protein transport into and within chloroplasts
journal, February 1990
- Smeekens, Sjef; Weisbeek, Peter; Robinson, Colin
- Trends in Biochemical Sciences, Vol. 15, Issue 2, p. 73-76
Self Processing Plants and Plant Parts
patent-application, December 2009
- Lanahan, Michael B.; Basu, Shib S.; Batie, Christopher J.
- US Patent Application 12/544546; 20090320831
Formulation for treating silage containing .beta.-1,4-xylanase and .beta.-1,3-xylosidase but essentially free of .beta.-1,4-glucanase and .beta.-1,4-cellobiohydrolase, and one or more lactic acid-producing bacteria
patent, July 1995
- Evans, Christopher T.; Mann, Stephen Philip; Charley, Robert C.
- US Patent Document 5,432,074
Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.
journal, November 1997
- Smith, D. R.; Doucette-Stamm, L. A.; Deloughery, C.
- Journal of Bacteriology, Vol. 179, Issue 22, p. 7135-7155
Heterologous Acidothermus cellulolyticus 1,4-β-endoglucanase E1 produced within the corn biomass converts corn stover into glucose
journal, April 2007
- Ransom, Callista; Balan, Venkatesh; Biswas, Gadab
- Applied Biochemistry and Biotechnology, Vol. 137-140, Issue 1-12, p. 207-219
The use of phosphomannose-isomerase as a selectable marker to recover transgenic maize plants ( Zea mays L.) via Agrobacterium transformation
journal, July 2000
- Negrotto, D.; Jolley, M.; Beer, S.
- Plant Cell Reports, Vol. 19, Issue 8, p. 798-803
Fermentation of lignocellulosic hydrolysates for ethanol production
journal, April 1996
- Olsson, Lisbeth; Hahn-Hägerdal, Bärbel
- Enzyme and Microbial Technology, Vol. 18, Issue 5, p. 312-331
Characterization of the Escherichia coli RNA 3′-Terminal Phosphate Cyclase and Its ς 54 -Regulated Operon
journal, September 1998
- Genschik, Pascal; Drabikowski, Krzysztof; Filipowicz, Witold
- Journal of Biological Chemistry, Vol. 273, Issue 39
Segmental Isotope Labeling for Protein NMR Using Peptide Splicing
journal, June 1998
- Yamazaki, Toshio; Otomo, Takanori; Oda, Natsuko
- Journal of the American Chemical Society, Vol. 120, Issue 22
Protein trans-splicing in transgenic plant chloroplast: Reconstruction of herbicide resistance from split genes
journal, April 2003
- Chin, H. G.; Kim, G.-D.; Marin, I.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 8, p. 4510-4515
Methods for Increasing Starch Content in Plant Cobs
patent-application, March 2012
- Steffens, John
- US Patent Application 13/203231; 20120054915
Enzymatic deconstruction of xylan for biofuel production
journal, February 2009
- Dodd, Dylan; Cann, Isaac K. O.
- GCB Bioenergy, Vol. 1, Issue 1
Compositions and methods for enhancing the degradation or conversion of cellulose-containing material
patent-application, June 2010
- McFarland, Keith
- US Patent Application 12/598574; 20100143967
In vitro protein splicing of purified precursor and the identification of a branched intermediate
journal, December 1993
- Xu, Ming-Qun; Southworth, Maurice W.; Mersha, Fana B.
- Cell, Vol. 75, Issue 7
A proposed mechanism for the self-splicing of proteins.
journal, November 1994
- Clarke, N. D.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 23
Protein splicing ? the lengths some proteins will go to
journal, January 1995
- Davis, Elaine O.; Jenner, Peter J.
- Antonie van Leeuwenhoek, Vol. 67, Issue 2
The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum
journal, September 2000
- Ruepp, Andreas; Graml, Werner; Santos-Martinez, Martha-Leticia
- Nature, Vol. 407, Issue 6803, p. 508-513
Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them
patent, January 2013
- Burke, Ellen; Cayouette, Michelle; Chang, Cathy
- US Patent Document 8,343,747
Transgenic plants expressing CIVPS or intein modified proteins and related method
patent, March 2011
- Raab, R. Michael
- US Patent Document 7,906,704
Fertile transgenic maize plants containing a gene encoding the pat protein
patent, May 2002
- Mumm, Rita; Spencer, T. Michael
- US Patent Document 6,395,966
The mechanism of protein splicing and its modulation by mutation.
journal, October 1996
- Xu, M. Q.; Perler, F. B.
- The EMBO Journal, Vol. 15, Issue 19
Plants with reduced activity of a starch phosphorylating enzyme
patent, September 2012
- Frohberg, Claus; Koetting, Oliver; Ritte, Gerhard
- US Patent Document 8,257,502
Biorefineries: Current Status, Challenges, and Future Direction
journal, July 2006
- Fernando, Sandun; Adhikari, Sushil; Chandrapal, Chauda
- Energy & Fuels, Vol. 20, Issue 4, p. 1727-1737
Cloning of the xynB Gene fromDictyoglomus thermophilum Rt46B.1 and Action of the Gene Product on Kraft Pulp
journal, May 1998
- Morris, Daniel D.; Gibbs, Moreland D.; Chin, Charles W. J.
- Applied and Environmental Microbiology, Vol. 64, Issue 5
Invasion of a multitude of genetic niches by mobile endonuclease genes
journal, April 2000
- Gimble, Frederick S.
- FEMS Microbiology Letters, Vol. 185, Issue 2
Multi-gene expression constructs containing modified inteins
patent, June 2010
- Snell, Kristi D.
- US Patent Document 7,741,530
Control of protein splicing by intein fragment reassembly
journal, February 1998
- Southworth, Maurice W.; Adam, Eric; Panne, Daniel
- The EMBO Journal, Vol. 17, Issue 4, p. 918-926
Intein-Modified Enzymes, Their Production and Industrial Applications
patent-application, May 2011
- Shen, Binzhang; Lazar, Gabor; De La Vega, Hunberto
- US Patent Application 12/590444; 20110111442
Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
patent, November 1999
- Austin-Phillips, Sandra; Burgess, Richard; German, Thomas
- US Patent Document 5,981,835
Cloning and characterisation of a thermostable α-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY
journal, December 1997
- Niehaus, Frank; Frey, Bruno; Antranikian, Garabed
- Gene, Vol. 204, Issue 1-2, p. 153-158
In vitro splicing of erythropoietin by the Mycobacterium tuberculosis RecA intein without substituting amino acids at the splice junctions
journal, January 2003
- Gangopadhyay, Jaya Pal; Jiang, Shu-qin; van Berkel, Patrick
- Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1619, Issue 2
Structural features affecting biomass enzymatic digestibility
journal, June 2008
- Zhu, Li; O’Dwyer, Jonathan P.; Chang, Vincent S.
- Bioresource Technology, Vol. 99, Issue 9, p. 3817-3828
Protein Splicing: Evidence for an N−O Acyl Rearrangement as the Initial Step in the Splicing Process
journal, January 1996
- Shao, Yang; Xu, Ming-Qun; Paulus, Henry
- Biochemistry, Vol. 35, Issue 12, p. 3810-3815
Intervening sequences in an Archaea DNA polymerase gene.
journal, June 1992
- Perler, F. B.; Comb, D. G.; Jack, W. E.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 12, p. 5577-5581
Method for performing homologous recombination in plants
patent, June 2013
- Paul, Wyatt; Wehrkamp-Richter, Sophie; Laffaire, Jean-Baptiste
- US Patent Document 8,455,715
Intein-mediated assembly of a functional β-glucuronidase in transgenic plants
journal, March 2003
- Yang, J.; Fox, G. C.; Henry-Smith, T. V.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 6, p. 3513-3518
Autohydrolysis of plant xylans by apoplastic expression of thermophilic bacterial endo-xylanases
journal, April 2010
- Borkhardt, Bernhard; Harholt, Jesper; Ulvskov, Peter
- Plant Biotechnology Journal, Vol. 8, Issue 3
Reactivity of the Cysteine Residues in the Protein Splicing Active Center of the Mycobacterium tuberculosis RecA Intein
journal, March 2000
- Shingledecker, Kaori; Jiang, Shu-qin; Paulus, Henry
- Archives of Biochemistry and Biophysics, Vol. 375, Issue 1, p. 138-144
Xylanse variants having altered sensitivity to xylanse inhibitors
patent-application, September 2003
- Sibbesen, Ole; Sorensen, Jens Frisbaek
- US Patent Application 10/237386; 20030180895
Improved segmental isotope labeling of proteins and application to a larger protein
journal, January 1999
- Otomo, Takanori; Teruya, Kenta; Uegaki, Koichi
- Journal of Biomolecular NMR, Vol. 14, Issue 2, p. 105-114
Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens
journal, May 2006
- Hiei, Yukoh; Komari, Toshihiko
- Plant Cell, Tissue and Organ Culture, Vol. 85, Issue 3
Self processing plants and plant parts
patent, December 2010
- Lanahan, Michael B.; Basu, Shib Sankar; Batie, Christopher J.
- US Patent Document 7,855,322
Production of Correctly Processed Human Serum Albumin in Transgenic Plants
journal, March 1990
- Sijmons, Peter C.; Dekker, Ben M. M.; Schrammeijer, Barbara
- Nature Biotechnology, Vol. 8, Issue 3, p. 217-221
Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants
journal, November 2006
- Biswas, Gadab C. Ghosh; Ransom, Callista; Sticklen, Mariam
- Plant Science, Vol. 171, Issue 5, p. 617-623
Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
patent, May 2006
- Sticklen, Masomeh B.; Dale, Bruce E.; Maqbool, Shahina B.
- US Patent Document 7,049,485
Conserved sequence features of inteins (protein introns) and their use in identifying new inteins and related proteins
journal, December 1994
- Pietrokovski, Shmuel
- Protein Science, Vol. 3, Issue 12, p. 2340-2350
Towards a comparative anatomy of N-terminal topogenic protein sequences
journal, May 1986
- von Heijne, Gunnar
- Journal of Molecular Biology, Vol. 189, Issue 1, p. 239-242
Oral immunization by transgenic plants
patent, August 1997
- Curtiss, III, Roy; Cardineau, Guy A.
- US Patent Document 5,654,184
Plants with Modified Starch Metabolism
patent-application, February 2011
- Ral, Jean-Philippe Francois Michel; Li, Zhongyi; Morell, Matthew Kennedy
- US Patent Application 12/745237; 20110045127
Pretreatment of woody biomass for biofuel production: energy efficiency, technologies, and recalcitrance
journal, May 2010
- Zhu, J. Y.; Pan, Xuejun; Zalesny, Ronald S.
- Applied Microbiology and Biotechnology, Vol. 87, Issue 3
Bioconversion of starch into ethanol by a recombinant Saccharomyces cerevisiae strain YPG-AB
journal, May 2002
- Ülgen, Kutluö Ö; Saygılı, Başak; Önsan, Z. İlsen
- Process Biochemistry, Vol. 37, Issue 10
Modular organization of inteins and C-terminal autocatalytic domains
journal, January 1998
- Pietrokovski, Shmuel
- Protein Science, Vol. 7, Issue 1, p. 64-71
PLANT TRANSFORMATION: Problems and Strategies for Practical Application
journal, June 1997
- Birch, R. G.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 48, Issue 1
pBINPLUS/ARS: an improved plant transformation vector based on pBINPLUS
journal, May 2008
- Belknap, William R.; Rockhold, David R.; McCue, Kent F.
- BioTechniques, Vol. 44, Issue 6
Modification of protein by use of a controllable interveining protein sequence
patent, March 1996
- Comb, Donald G.; Perler, Francine B.; Jack, William E.
- US Patent Document 5,496,714