Title: Rapid microfluidic thermal cycler for nucleic acid amplification

Abstract

A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.

Inventors:

Beer, Neil Reginald; Vafai, Kambiz

Issue Date:

Tue Oct 27 00:00:00 EDT 2015

Research Org.:

Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1224444

Patent Number(s):

9170060

Application Number:

12/270,348

Assignee:

Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

F - MECHANICAL ENGINEERING F28 - HEAT EXCHANGE IN GENERAL F28F - DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2300/0636 - Integrated biosensor, microarrays
B01L2300/0816 - Cards, e.g. flat sample carriers usually with flow in two horizontal directions
B01L2300/0838 - Capillaries
B01L2300/1838 - using fluid heat transfer medium
B01L2300/185 - using a liquid as fluid
B01L3/5023 - {with a sample being transported to, and subsequently stored in an absorbent for analysis}
B01L3/502784 - {specially adapted for droplet or plug flow, e.g. digital microfluidics
B01L7/52 - {with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

F - MECHANICAL ENGINEERING F28 - HEAT EXCHANGE IN GENERAL F28F - DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION

F28F13/003 - {by using permeable mass, perforated or porous materials
F28F2260/02 - having microchannels
F28F3/12 - Elements constructed in the shape of a hollow panel, e.g. with channels {

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DOE Contract Number:  

AC52-07NA27344

Resource Type:

Patent

Resource Relation:

Patent File Date: 2008 Nov 13

Country of Publication:

United States

Language:

English

Subject:

42 ENGINEERING