High density growth of T7 expression strains with auto-induction option

Studier, F. William

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Title: High density growth of T7 expression strains with auto-induction option

Abstract

Methods for promoting and suppressing auto-induction of transcription of cloned DNA in cultures of T7 expression strains are disclosed.

Inventors:: Studier, F. William

Issue Date:: Tue Apr 27 00:00:00 EDT 2010

Research Org.:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1176255

Patent Number(s):: 7704722

Application Number:: 11/741,048

Assignee:: Brookhaven Science Associates, LLC (Upton, NY)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N1/20 - Bacteria {
C12N15/72 - Expression systems using regulatory sequences derived from the lac-operon

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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DOE Contract Number:: AC02-98CH10886

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats


                    Studier, F. William. High density growth of T7 expression strains with auto-induction option.  United States: N. p., 2010. 
        Web.

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                    Studier, F. William. High density growth of T7 expression strains with auto-induction option.  United States.

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                    Studier, F. William. Tue .  
        "High density growth of T7 expression strains with auto-induction option".  United States.  https://www.osti.gov/servlets/purl/1176255.

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@article{osti_1176255,

  title        = {High density growth of T7 expression strains with auto-induction option},

  author       = {Studier, F. William},

  abstractNote = {Methods for promoting and suppressing auto-induction of transcription of cloned DNA in cultures of T7 expression strains are disclosed.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Apr 27 00:00:00 EDT 2010},

  month        = {Tue Apr 27 00:00:00 EDT 2010}

}

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Works referenced in this record:

Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability
journal, March 1998

Grossman, Trudy H.; Kawasaki, Ernest S.; Punreddy, Sandhya R.
Gene, Vol. 209, Issue 1-2
https://doi.org/10.1016/S0378-1119(98)00020-1

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor
journal, May 1991

Dubendorf, John W.; Studier, F. William
Journal of Molecular Biology, Vol. 219, Issue 1
https://doi.org/10.1016/0022-2836(91)90856-2

The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep
journal, March 2005

Grabski, Anthony; Mehler, Mark; Drott, Don
Nature Methods, Vol. 2, Issue 3
https://doi.org/10.1038/nmeth0305-233

Protein production in Escherichia coli for structural studies by X-ray crystallography
journal, April 2003

Goulding, Celia W.; Jeanne Perry, L.
Journal of Structural Biology, Vol. 142, Issue 1
https://doi.org/10.1016/S1047-8477(03)00044-3

The General Stress Sigma Factor ςS ofEscherichia coli Is Induced during Diauxic Shift from Glucose to Lactose
journal, January 1998

Fischer, Daniela; Teich, Antje; Neubauer, Peter
Journal of Bacteriology, Vol. 180, Issue 23
https://doi.org/10.1128/.180.23.6203-6206.1998

cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli
journal, November 1997

Kimata, K.; Takahashi, H.; Inada, T.
Proceedings of the National Academy of Sciences, Vol. 94, Issue 24
https://doi.org/10.1073/pnas.94.24.12914

Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination
journal, April 2005

Tyler, Robert C.; Sreenath, Hassan K.; Singh, Shanteri
Protein Expression and Purification, Vol. 40, Issue 2
https://doi.org/10.1016/j.pep.2004.12.024

Protein production by auto-induction in high-density shaking cultures
journal, May 2005

Studier, F. William
Protein Expression and Purification, Vol. 41, Issue 1, p. 207-234
https://doi.org/10.1016/j.pep.2005.01.016

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
journal, May 1986

Studier, F. William; Moffatt, Barbara A.
Journal of Molecular Biology, Vol. 189, Issue 1, p. 113-130
https://doi.org/10.1016/0022-2836(86)90385-2

Stability of plasmid and expression of a recombinant gonadotropin-releasing hormone (GnRH) vaccine in Escherichia coli
journal, December 2006

Xu, Jinshu; Li, Wenjia; Wu, Jie
Applied Microbiology and Biotechnology, Vol. 73, Issue 4
https://doi.org/10.1007/s00253-006-0547-7

[6] Use of T7 RNA polymerase to direct expression of cloned genes
book, January 1990

William Studier, F.; Rosenberg, Alan H.; Dunn, John J.
Methods in Enzymology
https://doi.org/10.1016/0076-6879(90)85008-C

Fermentation conditions for high-level expression of the tac -promoter-controlled calf prochymosin cDNA in Escherichia coli HB101: FERMENTATION CONDITIONS FOR HIGH-LEVEL EXPRESSION
journal, January 1991

Kaprálek, F.; Ječmen, P.; Sedláček, J.
Biotechnology and Bioengineering, Vol. 37, Issue 1
https://doi.org/10.1002/bit.260370111

Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium
journal, April 2005

Sreenath, Hassan K.; Bingman, Craig A.; Buchan, Blake W.
Protein Expression and Purification, Vol. 40, Issue 2
https://doi.org/10.1016/j.pep.2004.12.022

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High density growth of T7 expression strains with auto-induction option

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Disclosed is a method for promoting auto-induction of transcription of cloned DNA in cultures of bacterial cells grown batchwise, the transcription being under the control of a promoter whose activity can be induced by an exogenous inducer whose ability to induce said promoter is dependent on the metabolic state of said bacterial cells. Initially, a culture media is provided which includes: i) an inducer that causes induction of transcription from said promoter in said bacterial cells; and ii) a metabolite that prevents induction by said inducer, the concentration of said metabolite being adjusted so as to substantially preclude induction bymore » « less
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A method for promoting and suppressing auto-induction of transcription of a cloned gene 1 of bacteriophage T7 in cultures of bacterial cells grown batchwise is disclosed. The transcription is under the control of a promoter whose activity can be induced by an exogenous inducer whose ability to induce said promoter is dependent on the metabolic state of said bacterial cells.
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Similar Records

Title: High density growth of T7 expression strains with auto-induction option

Abstract

Citation Formats

Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability journal, March 1998

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor journal, May 1991

The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep journal, March 2005

Protein production in Escherichia coli for structural studies by X-ray crystallography journal, April 2003

The General Stress Sigma Factor ςS ofEscherichia coli Is Induced during Diauxic Shift from Glucose to Lactose journal, January 1998

cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli journal, November 1997

Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination journal, April 2005

Protein production by auto-induction in high-density shaking cultures journal, May 2005

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes journal, May 1986

Stability of plasmid and expression of a recombinant gonadotropin-releasing hormone (GnRH) vaccine in Escherichia coli journal, December 2006

[6] Use of T7 RNA polymerase to direct expression of cloned genes book, January 1990

Fermentation conditions for high-level expression of the tac -promoter-controlled calf prochymosin cDNA in Escherichia coli HB101: FERMENTATION CONDITIONS FOR HIGH-LEVEL EXPRESSION journal, January 1991

Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium journal, April 2005

Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability
journal, March 1998

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor
journal, May 1991

The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep
journal, March 2005

Protein production in Escherichia coli for structural studies by X-ray crystallography
journal, April 2003

The General Stress Sigma Factor ςS ofEscherichia coli Is Induced during Diauxic Shift from Glucose to Lactose
journal, January 1998

cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli
journal, November 1997

Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination
journal, April 2005

Protein production by auto-induction in high-density shaking cultures
journal, May 2005

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
journal, May 1986

Stability of plasmid and expression of a recombinant gonadotropin-releasing hormone (GnRH) vaccine in Escherichia coli
journal, December 2006

[6] Use of T7 RNA polymerase to direct expression of cloned genes
book, January 1990

Fermentation conditions for high-level expression of the tac -promoter-controlled calf prochymosin cDNA in Escherichia coli HB101: FERMENTATION CONDITIONS FOR HIGH-LEVEL EXPRESSION
journal, January 1991

Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium
journal, April 2005