Method to amplify variable sequences without imposing primer sequences
Abstract
The present invention provides methods of amplifying target sequences without including regions flanking the target sequence in the amplified product or imposing amplification primer sequences on the amplified product. Also provided are methods of preparing a library from such amplified target sequences.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175987
- Patent Number(s):
- 7135310
- Application Number:
- 10/167,634
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-98ER62647
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2002 Jun 11
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 97 MATHEMATICS AND COMPUTING
Citation Formats
Bradbury, Andrew M., and Zeytun, Ahmet. Method to amplify variable sequences without imposing primer sequences. United States: N. p., 2006.
Web.
Bradbury, Andrew M., & Zeytun, Ahmet. Method to amplify variable sequences without imposing primer sequences. United States.
Bradbury, Andrew M., and Zeytun, Ahmet. Tue .
"Method to amplify variable sequences without imposing primer sequences". United States. https://www.osti.gov/servlets/purl/1175987.
@article{osti_1175987,
title = {Method to amplify variable sequences without imposing primer sequences},
author = {Bradbury, Andrew M. and Zeytun, Ahmet},
abstractNote = {The present invention provides methods of amplifying target sequences without including regions flanking the target sequence in the amplified product or imposing amplification primer sequences on the amplified product. Also provided are methods of preparing a library from such amplified target sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 14 00:00:00 EST 2006},
month = {Tue Nov 14 00:00:00 EST 2006}
}
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Structure and function of type II restriction endonucleases
journal, September 2001
- Pingoud, A.
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