Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms
Abstract
A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.
- Inventors:
- Issue Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175631
- Patent Number(s):
- 6989252
- Application Number:
- 09/748,690
- Assignee:
- Midwest Research Institute (Kansas City, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC36-99GO10337
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Melis, Anastasios, Zhang, Liping, Benemann, John R., Forestier, Marc, Ghirardi, Maria, and Seibert, Michael. Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms. United States: N. p., 2006.
Web.
Melis, Anastasios, Zhang, Liping, Benemann, John R., Forestier, Marc, Ghirardi, Maria, & Seibert, Michael. Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms. United States.
Melis, Anastasios, Zhang, Liping, Benemann, John R., Forestier, Marc, Ghirardi, Maria, and Seibert, Michael. Tue .
"Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms". United States. https://www.osti.gov/servlets/purl/1175631.
@article{osti_1175631,
title = {Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms},
author = {Melis, Anastasios and Zhang, Liping and Benemann, John R. and Forestier, Marc and Ghirardi, Maria and Seibert, Michael},
abstractNote = {A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 24 00:00:00 EST 2006},
month = {Tue Jan 24 00:00:00 EST 2006}
}
Works referenced in this record:
Hydrogen biotechnology: Progress and prospects
journal, September 1996
- Benemann, John
- Nature Biotechnology, Vol. 14, Issue 9
Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga Chlamydomonas reinhardtii
journal, January 2000
- Melis, Anastasios; Zhang, Liping; Forestier, Marc
- Plant Physiology, Vol. 122, Issue 1