DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis

Abstract

The present invention comprises a Mytilus edulis cDNA sequenc having a nucleotide sequence that encodes for the Mytilus edulis foot protein-1 (Mefp-1), an example of a mollusk foot protein. Mefp-1 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-1 gene will allow researchers to produce Mefp-1 protein using genetic engineering techniques. The discovery of Mefp-1 gene sequence will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.

Inventors:
;
Issue Date:
Research Org.:
Idaho National Laboratory (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175628
Patent Number(s):
6987170
Application Number:
10/915,161
Assignee:
Battelle Energy Alliance, LLC (Idaho Falls, ID)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC07-99JD13727
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Silverman, Heather G., and Roberto, Francisco F. Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis. United States: N. p., 2006. Web.
Silverman, Heather G., & Roberto, Francisco F. Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis. United States.
Silverman, Heather G., and Roberto, Francisco F. Tue . "Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis". United States. https://www.osti.gov/servlets/purl/1175628.
@article{osti_1175628,
title = {Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis},
author = {Silverman, Heather G. and Roberto, Francisco F.},
abstractNote = {The present invention comprises a Mytilus edulis cDNA sequenc having a nucleotide sequence that encodes for the Mytilus edulis foot protein-1 (Mefp-1), an example of a mollusk foot protein. Mefp-1 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-1 gene will allow researchers to produce Mefp-1 protein using genetic engineering techniques. The discovery of Mefp-1 gene sequence will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 17 00:00:00 EST 2006},
month = {Tue Jan 17 00:00:00 EST 2006}
}

Works referenced in this record:

Adhesion and substrate choice in mussels and barnacles
journal, March 1985


Expression Sites of Two Byssal Protein Genes of Mytilus galloprovincialis
journal, April 1996


Preliminary Evaluation of the Use of Mussel Adhesive Protein in Experimental Epikeratoplasty
journal, July 1988


Mussel glue fromMytilus californianus Conrad: a comparative study
journal, January 1986


Mussel Adhesive Plaque Protein Gene Is a Novel Member of Epidermal Growth Factor-like Gene Family
journal, March 1995


Metal-Mediated Cross-Linking in the Generation of a Marine-Mussel Adhesive
journal, January 2004


Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes.
journal, March 1990


Characterization of a Cystine-Rich Polyphenolic Protein Family from the Blue Mussel Mytilus edulis L
journal, August 1992


Mussel Adhesive Enhances the Immobilization of Human Chorionic Gonadotrophin to a Solid Support
journal, October 1996


Role of l -3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins
journal, June 1999


Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997


Attachment of epiphyseal cartilage cells and 17/28 rat osteosarcoma osteoblasts using mussel adhesive protein
journal, November 1990


Minireview-Polyphenols and Oxidases in Substratum Adhesion by Marine Algae and Mussels
journal, February 1998


Expression of multiple forms of an adhesive plaque protein in an individual mussel, Mytilus edulis
journal, September 1999


Reverse Engineering of Bioadhesion in Marine Mussels
journal, June 1999


A potential mediator of collagenous block copolymer gradients in mussel byssal threads
journal, September 1998


Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993


The strength of adhesive-bonded tissue joints
journal, May 1997


The phylogeny and chemical diversity of quinone-tanned glues and varnishes
journal, January 1990


Polyphosphoprotein from the Adhesive Pads of Mytilus edulis
journal, March 2001


Hydroxyarginine-containing Polyphenolic Proteins in the Adhesive Plaques of the Marine Mussel Mytilus edulis
journal, August 1995