PCR amplification on microarrays of gel immobilized oligonucleotides
Abstract
The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Chicago, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1174559
- Patent Number(s):
- 6642000
- Application Number:
- 09/559,306
- Assignee:
- University Of Chicago
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-31-1-0-ENG-39
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; 42 ENGINEERING
Citation Formats
Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, and Mirzabekov, Andrei. PCR amplification on microarrays of gel immobilized oligonucleotides. United States: N. p., 2003.
Web.
Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, & Mirzabekov, Andrei. PCR amplification on microarrays of gel immobilized oligonucleotides. United States.
Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, and Mirzabekov, Andrei. Tue .
"PCR amplification on microarrays of gel immobilized oligonucleotides". United States. https://www.osti.gov/servlets/purl/1174559.
@article{osti_1174559,
title = {PCR amplification on microarrays of gel immobilized oligonucleotides},
author = {Strizhkov, Boris and Tillib, Sergei and Mikhailovich, Vladimir and Mirzabekov, Andrei},
abstractNote = {The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 04 00:00:00 EST 2003},
month = {Tue Nov 04 00:00:00 EST 2003}
}
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