Microbial production of epoxides
Abstract
A method for microbial production of epoxides and other oxygenated products is disclosed. The method uses a biocatalyst of methanotrophic bacteria cultured in a biphasic medium containing a major amount of a non-aqueous polar solvent. Regeneration of reducing equivalents is carried out by using endogenous hydrogenase activity together with supplied hydrogen gas. This method is especially effective with gaseous substrates and cofactors that result in liquid products.
- Inventors:
- Issue Date:
- Research Org.:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1174365
- Patent Number(s):
- 6576449
- Application Number:
- 09/805,795
- Assignee:
- Bechtel BWXT Idaho, LLC (Idaho Falls, ID)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC07-94ID13223
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Clark, Thomas R., and Roberto, Francisco F. Microbial production of epoxides. United States: N. p., 2003.
Web.
Clark, Thomas R., & Roberto, Francisco F. Microbial production of epoxides. United States.
Clark, Thomas R., and Roberto, Francisco F. Tue .
"Microbial production of epoxides". United States. https://www.osti.gov/servlets/purl/1174365.
@article{osti_1174365,
title = {Microbial production of epoxides},
author = {Clark, Thomas R. and Roberto, Francisco F.},
abstractNote = {A method for microbial production of epoxides and other oxygenated products is disclosed. The method uses a biocatalyst of methanotrophic bacteria cultured in a biphasic medium containing a major amount of a non-aqueous polar solvent. Regeneration of reducing equivalents is carried out by using endogenous hydrogenase activity together with supplied hydrogen gas. This method is especially effective with gaseous substrates and cofactors that result in liquid products.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 10 00:00:00 EDT 2003},
month = {Tue Jun 10 00:00:00 EDT 2003}
}
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