Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Mayfield, Stephen P

Advanced Search OptionsAdvanced Search queries use a traditional Term Search. For more info, see our FAQ.

All Fields:

Patent Title:

Abstract:

Assignee:

Inventor(s):

Patent Number:

Patent Classification (CPC):

All Classifications
A - human necessities
A01 - agriculture
A21 - baking
A22 - butchering
A23 - foods or foodstuffs
A24 - tobacco
A41 - wearing apparel
A42 - headwear
A43 - footwear
A44 - haberdashery
A45 - hand or travelling articles
A46 - brushware
A47 - furniture
A61 - medical or veterinary science
A62 - life-saving
A63 - sports
A99 - subject matter not otherwise provided for in this section
B - performing operations
B01 - physical or chemical processes or apparatus in general
B02 - crushing, pulverising, or disintegrating
B03 - separation of solid materials using liquids or using pneumatic tables or jigs
B04 - centrifugal apparatus or machines for carrying-out physical or chemical processes
B05 - spraying or atomising in general
B06 - generating or transmitting mechanical vibrations in general
B07 - separating solids from solids
B08 - cleaning
B09 - disposal of solid waste
B21 - mechanical metal-working without essentially removing material
B22 - casting
B23 - machine tools
B24 - grinding
B25 - hand tools
B26 - hand cutting tools
B27 - working or preserving wood or similar material
B28 - working cement, clay, or stone
B29 - working of plastics
B30 - presses
B31 - making articles of paper, cardboard or material worked in a manner analogous to paper
B32 - layered products
B33 - additive manufacturing technology
B41 - printing
B42 - bookbinding
B43 - writing or drawing implements
B44 - decorative arts
B60 - vehicles in general
B61 - railways
B62 - land vehicles for travelling otherwise than on rails
B63 - ships or other waterborne vessels
B64 - aircraft
B65 - conveying
B66 - hoisting
B67 - opening, closing {or cleaning} bottles, jars or similar containers
B68 - saddlery
B81 - microstructural technology
B82 - nanotechnology
B99 - subject matter not otherwise provided for in this section
C - chemistry
C01 - inorganic chemistry
C02 - treatment of water, waste water, sewage, or sludge
C03 - glass
C04 - cements
C05 - fertilisers
C06 - explosives
C07 - organic chemistry
C08 - organic macromolecular compounds
C09 - dyes
C10 - petroleum, gas or coke industries
C11 - animal or vegetable oils, fats, fatty substances or waxes
C12 - biochemistry
C13 - sugar industry
C14 - skins
C21 - metallurgy of iron
C22 - metallurgy
C23 - coating metallic material
C25 - electrolytic or electrophoretic processes
C30 - crystal growth
C40 - combinatorial technology
C99 - subject matter not otherwise provided for in this section
D - textiles
D01 - natural or man-made threads or fibres
D02 - yarns
D03 - weaving
D04 - braiding
D05 - sewing
D06 - treatment of textiles or the like
D07 - ropes
D10 - indexing scheme associated with sublasses of section d, relating to textiles
D21 - paper-making
D99 - subject matter not otherwise provided for in this section
E - fixed constructions
E01 - construction of roads, railways, or bridges
E02 - hydraulic engineering
E03 - water supply
E04 - building
E05 - locks
E06 - doors, windows, shutters, or roller blinds in general
E21 - earth drilling
E99 - subject matter not otherwise provided for in this section
F - mechanical engineering
F01 - machines or engines in general
F02 - combustion engines
F03 - machines or engines for liquids
F04 - positive - displacement machines for liquids
F05 - indexing schemes relating to engines or pumps in various subclasses of classes f01-f04
F15 - fluid-pressure actuators
F16 - engineering elements and units
F17 - storing or distributing gases or liquids
F21 - lighting
F22 - steam generation
F23 - combustion apparatus
F24 - heating
F25 - refrigeration or cooling
F26 - drying
F27 - furnaces
F28 - heat exchange in general
F41 - weapons
F42 - ammunition
F99 - subject matter not otherwise provided for in this section
G - physics
G01 - measuring
G02 - optics
G03 - photography
G04 - horology
G05 - controlling
G06 - computing
G07 - checking-devices
G08 - signalling
G09 - education
G10 - musical instruments
G11 - information storage
G12 - instrument details
G16 - information and communication technology [ict] specially adapted for specific application fields
G21 - nuclear physics
G99 - subject matter not otherwise provided for in this section
H - electricity
H01 - basic electric elements
H02 - generation
H03 - basic electronic circuitry
H04 - electric communication technique
H05 - electric techniques not otherwise provided for
H99 - subject matter not otherwise provided for in this section
Y - new / cross sectional technologies
Y02 - technologies or applications for mitigation or adaptation against climate change
Y04 - information or communication technologies having an impact on other technology areas
Y10 - technical subjects covered by former uspc

More Options ...

Title: Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Abstract

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.

Inventors:: Mayfield, Stephen P

Issue Date:: Tue Jan 13 00:00:00 EST 2015

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1167243

Patent Number(s):: 8932576

Application Number:: 12/695,101

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A61P31/00 - Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/79 - Vectors or expression systems specially adapted for eukaryotic hosts
C12N15/8214 - {Plastid transformation}
C12N15/8257 - {for the production of primary gene products, e.g. pharmaceutical products, interferon}

Show less

DOE Contract Number:: FG03-02ER15313

Resource Type:: Patent

Resource Relation:: Patent File Date: 2010 Jan 27

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast.  United States: N. p., 2015. 
        Web.

Copy to clipboard


                    Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast.  United States.

Copy to clipboard


                    Mayfield, Stephen P. Tue .  
        "Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast".  United States.  https://www.osti.gov/servlets/purl/1167243.

Copy to clipboard


                    
@article{osti_1167243,

  title        = {Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast},

  author       = {Mayfield, Stephen P},

  abstractNote = {Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jan 13 00:00:00 EST 2015},

  month        = {Tue Jan 13 00:00:00 EST 2015}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Method for stably transforming plastids of multicellular plants
patent, September 1995

Maliga, Pal; Maliga, Zora S.
US Patent Document 5,451,513
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5451513

Enhanced expression in a plant plastid
patent, August 1996

McBride, Kevin E.; Stalker, David M.
US Patent Document 5,545,817
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5545817

Expression of Bacillus thuringiensis cry proteins in plant plastids
patent, August 1996

McBride, Kevin E.; Maliga, Pal
US Patent Document 5,545,818
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5545818

DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
patent, March 1999

Maliga, Pal; Maliga, Zora S.; Staub, Jeffrey M.
US Patent Document 5,877,402
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5877402

Genomic mammary Amyloid a sequence
patent, May 2007

McDonald, Thomas L.; Larson, Marilyn A.; Weber, Annika
US Patent Document 7,214,512
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7214512

Transgenic algae for delivering antigens to an animal
patent, August 2008

Sayre, Richard T.; Wagner, Richard E.; Siripornadulsil, Surasak
US Patent Document 7,410,637
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7410637

Participation of nuclear genes in chloroplast gene expression
journal, June 2000

Barkan, A.; Goldschmidtclermont, M.
Biochimie, Vol. 82, Issue 6-7
https://doi.org/10.1016/S0300-9084(00)00602-7

Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes
journal, October 2005

Barnes, Dwight; Franklin, Scott; Schultz, Jason
Molecular Genetics and Genomics, Vol. 274, Issue 6
https://doi.org/10.1007/s00438-005-0055-y

Transgenic Plastids in Basic Research and Plant Biotechnology
journal, September 2001

Bock, Ralph
Journal of Molecular Biology, Vol. 312, Issue 3
https://doi.org/10.1006/jmbi.2001.4960

Translational regulations as specific traits of chloroplast gene expression
journal, September 2002

Choquet, Yves; Wollman, Francis-André
FEBS Letters, Vol. 529, Issue 1, p. 39-42
https://doi.org/10.1016/S0014-5793(02)03260-X

Co-variation of tRNA Abundance and Codon Usage inEscherichia coliat Different Growth Rates
journal, August 1996

Dong, Hengjiang; Nilsson, Lars; Kurland, Charles G.
Journal of Molecular Biology, Vol. 260, Issue 5
https://doi.org/10.1006/jmbi.1996.0428

tRNA gene number and codon usage in the C. elegans genome are co-adapted for optimal translation of highly expressed genes
journal, July 2000

Duret, Laurent
Trends in Genetics, Vol. 16, Issue 7, p. 287-289
https://doi.org/10.1016/S0168-9525(00)02041-2

Consecutive Low-usage Leucine Codons Block Translation Only When Near the 5′ End of a Message inEscherichia coli
journal, February 1995

Goldman, Emanuel; Rosenberg, Alan H.; Zubay, Geoffrey
Journal of Molecular Biology, Vol. 245, Issue 5
https://doi.org/10.1006/jmbi.1994.0038

Evolution of organellar genomes
journal, December 1999

Gray, Michael W.
Current Opinion in Genetics & Development, Vol. 9, Issue 6, p. 678-687
https://doi.org/10.1016/S0959-437X(99)00030-1