Compositions and methods for improved protein production
Abstract
The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.
- Inventors:
- Issue Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1133651
- Patent Number(s):
- 8741654
- Application Number:
- 13/494,889
- Assignee:
- Danisco US Inc. (Palo Alto, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC36-99GO10337
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Bodie, Elizabeth A., and Kim, Steve Sungjin. Compositions and methods for improved protein production. United States: N. p., 2014.
Web.
Bodie, Elizabeth A., & Kim, Steve Sungjin. Compositions and methods for improved protein production. United States.
Bodie, Elizabeth A., and Kim, Steve Sungjin. Tue .
"Compositions and methods for improved protein production". United States. https://www.osti.gov/servlets/purl/1133651.
@article{osti_1133651,
title = {Compositions and methods for improved protein production},
author = {Bodie, Elizabeth A. and Kim, Steve Sungjin},
abstractNote = {The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 03 00:00:00 EDT 2014},
month = {Tue Jun 03 00:00:00 EDT 2014}
}
Works referenced in this record:
Hyperproducing cellulase microorganism
patent, September 1984
- Gallo, Benedict J.
- US Patent Document 4,472,504
Process for amplifying, detecting, and/or-cloning nucleic acid sequences
patent, July 1987
- Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman
- US Patent Document 4,683,195
Process for producing cellulolytic enzymes
patent, August 1988
- Warzywoda, Michel; Ferre, Veronique; Pourquie, Jacques
- US Patent Document 4,762,788
Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
patent, July 2001
- Beijersbergen, Alida Godelieve Maria; Bundock, Paul; Gouka, Robertus Johannes
- US Patent Document 6,255,115
Xylanase from trichoderma reesei, method for production thereof, and methods employing this enzyme
patent, July 2004
- Saloheimo, Markku; Siika-Aho, Matti; Tenkanen, Maija
- US Patent Document 6,768,001
Induction of gene expression using a high concentration sugar mixture
patent, May 2010
- England, George; Kelley, Aaron; Mitchinson, Colin
- US Patent Document 7,713,725
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
Strategies and applications of in vitro mutagenesis
journal, September 1985
- Botstein, D.; Shortle, D.
- Science, Vol. 229, Issue 4719
Genetic transformation of intact cells of Bacillus subtilis by electroporation
journal, January 1990
- Brigidi, P.; Rossi, E.; Bertarini, M.L.
- FEMS Microbiology Letters, Vol. 67, Issue 1-2, p. 135-138
Gene transfer to plants by diverse species of bacteria
journal, February 2005
- Broothaerts, Wim; Mitchell, Heidi J.; Weir, Brian
- Nature, Vol. 433, Issue 7026
Transformation of Trichoderma harzianum by high-voltage electric pulse
journal, February 1990
- Goldman, G. H.; Van Montagu, M.; Herrera-Estrella, A.
- Current Genetics, Vol. 17, Issue 2, p. 169-174
Transformation of Aspergillus awamori by Agrobacterium tumefaciens–mediated homologous recombination
journal, June 1999
- Gouka, Robin J.; Gerk, Casper; Hooykaas, Paul J. J.
- Nature Biotechnology, Vol. 17, Issue 6
The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
journal, September 1994
- Hajdukiewicz, Peter; Svab, Zora; Maliga, Pal
- Plant Molecular Biology, Vol. 25, Issue 6, p. 989-994
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions
journal, January 1988
- Higuchi, Russell; Krummel, Barbara; Saiki, Randall
- Nucleic Acids Research, Vol. 16, Issue 15
Site-directed mutagenesis by overlap extension using the polymerase chain reaction
journal, April 1989
- Ho, Steffan N.; Hunt, Henry D.; Horton, Robert M.
- Gene, Vol. 77, Issue 1, p. 51-59
NewAgrobacterium helper plasmids for gene transfer to plants
journal, July 1993
- Hood, Elizabeth E.; Gelvin, Stanton B.; Melchers, Leo S.
- Transgenic Research, Vol. 2, Issue 4
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
journal, April 1989
- Horton, Robert M.; Hunt, Henry D.; Ho, Steffan N.
- Gene, Vol. 77, Issue 1, p. 61-68
Plasmid transformation in Bacillus subtilis: Symmetry of gene conversion in transformation with a hybrid plasmid containing chromosomal DNA
journal, February 1983
- Iglesias, A.; Trautner, T. A.
- MGG Molecular & General Genetics, Vol. 189, Issue 1
Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking
journal, February 1995
- Liu, Yao-Guang; Whittier, Robert F.
- Genomics, Vol. 25, Issue 3, p. 674-681
Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR
journal, September 1995
- Liu, Yao-Guang; Mitsukawa, Norihiro; Oosumi, Teruko
- The Plant Journal, Vol. 8, Issue 3
Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA
journal, October 1993
- Lorito, M.; Hayes, C. K.; Di Pietro, A.
- Current Genetics, Vol. 24, Issue 4, p. 349-356
Primary structure of the trpC gene from Aspergillus nidulans
journal, January 1985
- Mullaney, Edward J.; Hamer, John E.; Roberti, Kellee A.
- MGG Molecular & General Genetics, Vol. 199, Issue 1, p. 37-45
Agrobacterium -Mediated Transformation of Fusarium oxysporum : An Efficient Tool for Insertional Mutagenesis and Gene Transfer
journal, February 2001
- Mullins, E. D.; Chen, X.; Romaine, P.
- Phytopathology, Vol. 91, Issue 2
A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970
- Needleman, Saul B.; Wunsch, Christian D.
- Journal of Molecular Biology, Vol. 48, Issue 3, p. 443-453
Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.
journal, November 1984
- Nunberg, J. H.; Meade, J. H.; Cole, G.
- Molecular and Cellular Biology, Vol. 4, Issue 11, p. 2306-2315
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
journal, January 1987
- Penttilä, Merja; Nevalainen, Helena; Rättö, Marjaana
- Gene, Vol. 61, Issue 2, p. 155-164
Cellulases of Trichoderma reesei
journal, January 1988
- Schülein, Martin
- Methods in Enzymology, Vol. 160, p. 234-242
Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation
journal, January 1991
- Smith, Jeffrey L.; Bayliss, Frank T.; Ward, Michael
- Current Genetics, Vol. 19, Issue 1
Comparison of biosequences
journal, December 1981
- Smith, Temple F.; Waterman, Michael S.
- Advances in Applied Mathematics, Vol. 2, Issue 4, p. 482-489
Successive Construction of Cellulase Hyperproducers of Trichoderma Using Hyperpolyploids
journal, January 2000
- Toyama, Hideo; Toyama, Nobuo
- Applied Biochemistry and Biotechnology, Vol. 84-86, Issue 1-9
Production of recombinant proteins in the filamentous fungus Trichoderma reesei
journal, January 1995
- Keränen, Sirkka; Penttilä, Merja
- Current Opinion in Biotechnology, Vol. 6, Issue 5, p. 534-537
Enhanced production of cellobiohydrolases in Trichoderma reesei and evaluation of the new preparations in biofinishing of cotton
journal, March 2005
- Miettinen-Oinonen, Arja; Paloheimo, Marja; Lantto, Raija
- Journal of Biotechnology, Vol. 116, Issue 3, p. 305-317
Construction of cellulase hyper-producers of Trichoderma reesei Rut C-30 for utilization of waste paper using colchicine and benomyl
journal, June 2002
- Toyama, Hideo; Yamagishi, Naotsugu; Toyama, Nobuo
- Journal of Molecular Catalysis B: Enzymatic, Vol. 17, Issue 3-5, p. 175-178
Works referencing / citing this record:
Protease deficient filamentous fungal cells and methods of use thereof
patent, February 2017
- Landowski, Christopher; Huuskonen, Anne; Saarinen, Juhani
- US Patent Document 9,567,596