Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus
Abstract
The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
- Inventors:
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1132775
- Patent Number(s):
- 8735562
- Application Number:
- 12/257,261
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2008 Oct 23
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Dai, Ziyu, Lasure, Linda L, and Magnuson, Jon K. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus. United States: N. p., 2014.
Web.
Dai, Ziyu, Lasure, Linda L, & Magnuson, Jon K. Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus. United States.
Dai, Ziyu, Lasure, Linda L, and Magnuson, Jon K. Tue .
"Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus". United States. https://www.osti.gov/servlets/purl/1132775.
@article{osti_1132775,
title = {Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus},
author = {Dai, Ziyu and Lasure, Linda L and Magnuson, Jon K},
abstractNote = {The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 27 00:00:00 EDT 2014},
month = {Tue May 27 00:00:00 EDT 2014}
}
Works referenced in this record:
Isolated polynucleotides and methods of promoting a morphology in a fungus
patent, October 2008
- Lasure, Linda L.; Dai, Ziyu
- US Patent Document 7,439,067
Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays
journal, August 1987
- Ebert, P. R.; Ha, S. B.; An, G.
- Proceedings of the National Academy of Sciences, Vol. 84, Issue 16