Title: Controllable assembly and disassembly of nanoparticle systems via protein and DNA agents

Abstract

The invention relates to the use of peptides, proteins, and other oligomers to provide a means by which normally quenched nanoparticle fluorescence may be recovered upon detection of a target molecule. Further, the inventive technology provides a structure and method to carry out detection of target molecules without the need to label the target molecules before detection. In another aspect, a method for forming arbitrarily shaped two- and three-dimensional protein-mediated nanoparticle structures and the resulting structures are described. Proteins mediating structure formation may themselves be functionalized with a variety of useful moieties, including catalytic functional groups.

Inventors:

Lee, Soo-Kwan; Gang, Oleg; van der Lelie, Daniel

Issue Date:

Tue May 20 00:00:00 EDT 2014

Research Org.:

Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1132075

Patent Number(s):

8729012

Application Number:

12/995,919

Assignee:

Brookhaven Science Associates, LLC (Upton, NY)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6816 - characterised by the detection means
C12Q2522/101 - Single or double stranded nucleic acid binding proteins
C12Q2563/155 - Particles of a defined size, e.g. nanoparticles

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/5434 - {using magnetic particle immunoreagent carriers which constitute new materials per se}
G01N33/54346 - {Nanoparticles}

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DOE Contract Number:  

AC02-98CH10886

Resource Type:

Patent

Resource Relation:

Patent File Date: 2009 Jun 02

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES