Method of detecting luminescent target ions with modified magnetic microspheres
Abstract
This invention provides methods of using modified magnetic microspheres to extract target ions from a sample in order to detect their presence in a microfluidic environment. In one or more embodiments, the microspheres are modified with molecules on the surface that allow the target ions in the sample to form complexes with specific ligand molecules on the microsphere surface. In one or more embodiments, the microspheres are modified with molecules that sequester the target ions from the sample, but specific ligand molecules in solution subsequently re-extract the target ions from the microspheres into the solution, where the complexes form independent of the microsphere surface. Once the complexes form, they are exposed to an excitation wavelength light source suitable for exciting the target ion to emit a luminescent signal pattern. Detection of the luminescent signal pattern allows for determination of the presence of the target ions in the sample.
- Inventors:
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1131022
- Patent Number(s):
- 8722415
- Application Number:
- 13/038,673
- Assignee:
- U.S. Department of Energy (Washington, DC)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC02-06CH11357
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2011 Mar 02
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Shkrob, Ilya A, and Kaminski, Michael D. Method of detecting luminescent target ions with modified magnetic microspheres. United States: N. p., 2014.
Web.
Shkrob, Ilya A, & Kaminski, Michael D. Method of detecting luminescent target ions with modified magnetic microspheres. United States.
Shkrob, Ilya A, and Kaminski, Michael D. Tue .
"Method of detecting luminescent target ions with modified magnetic microspheres". United States. https://www.osti.gov/servlets/purl/1131022.
@article{osti_1131022,
title = {Method of detecting luminescent target ions with modified magnetic microspheres},
author = {Shkrob, Ilya A and Kaminski, Michael D},
abstractNote = {This invention provides methods of using modified magnetic microspheres to extract target ions from a sample in order to detect their presence in a microfluidic environment. In one or more embodiments, the microspheres are modified with molecules on the surface that allow the target ions in the sample to form complexes with specific ligand molecules on the microsphere surface. In one or more embodiments, the microspheres are modified with molecules that sequester the target ions from the sample, but specific ligand molecules in solution subsequently re-extract the target ions from the microspheres into the solution, where the complexes form independent of the microsphere surface. Once the complexes form, they are exposed to an excitation wavelength light source suitable for exciting the target ion to emit a luminescent signal pattern. Detection of the luminescent signal pattern allows for determination of the presence of the target ions in the sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 13 00:00:00 EDT 2014},
month = {Tue May 13 00:00:00 EDT 2014}
}
Works referenced in this record:
Enzyme amplified, complex linked, competitive and non-competitive assays for the detection of metal ions
patent, October 1995
- Hammock, Bruce D.; Szurdoki, Ferenc; Kido, Horacio
- US Patent Document 5,459,040
Wavelength-coded bead for bioassay and signature recogniton
patent-application, November 2002
- Ho, Winston Z.
- US Patent Application 10/137461; 20020164271
Magnetic Extraction, Detection, and Isotope Analysis of Metal Ions Using Surface Modified Microspheres for Lab-on-a-Chip Applications
journal, January 2010
- Shkrob, Ilya A.; Kaminski, Michael D.; Rahimian, Kamyar
- Separation Science and Technology, Vol. 45, Issue 2
Sequestration, Fluorometric Detection, And Mass Spectroscopy Analysis of Lanthanide Ions Using Surface Modified Magnetic Microspheres for Microfluidic Manipulation
journal, November 2009
- Shkrob, Ilya A.; Kaminski, Michael D.; Mertz, Carol J.
- Journal of the American Chemical Society, Vol. 131, Issue 43