Selective posttranslational modification of phage-displayed polypeptides
Abstract
The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2]cycloaddition reactions and Staudinger modifications.
- Inventors:
- Issue Date:
- Research Org.:
- The Scripps Research Inst., La Jolla, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1083220
- Patent Number(s):
- 8367588
- Application Number:
- 11/992,341
- Assignee:
- The Scripps Research Institute (La Jolla, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Tsao, Meng-Lin, Tian, Feng, and Schultz, Peter. Selective posttranslational modification of phage-displayed polypeptides. United States: N. p., 2013.
Web.
Tsao, Meng-Lin, Tian, Feng, & Schultz, Peter. Selective posttranslational modification of phage-displayed polypeptides. United States.
Tsao, Meng-Lin, Tian, Feng, and Schultz, Peter. Tue .
"Selective posttranslational modification of phage-displayed polypeptides". United States. https://www.osti.gov/servlets/purl/1083220.
@article{osti_1083220,
title = {Selective posttranslational modification of phage-displayed polypeptides},
author = {Tsao, Meng-Lin and Tian, Feng and Schultz, Peter},
abstractNote = {The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2]cycloaddition reactions and Staudinger modifications.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 05 00:00:00 EST 2013},
month = {Tue Feb 05 00:00:00 EST 2013}
}
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