Title: Stochastic sensing through covalent interactions

Abstract

A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered P.sub.SH type or .alpha.HL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.

Inventors:

Bayley, Hagan; Shin, Seong-Ho; Luchian, Tudor; Cheley, Stephen

Issue Date:

Tue Mar 26 00:00:00 EDT 2013

Research Org.:

DOESC (USDOE Office of Science (SC) (United States))

Sponsoring Org.:

USDOE

OSTI Identifier:

1083020

Patent Number(s):

8404449

Application Number:

12/429,897

Assignee:

The Texas A&M University System (College Station, TX)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/001 - {Enzyme electrodes}

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/5308 - {for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites}
G01N33/5438 - {Electrodes}
G01N33/6872 - {Intracellular protein regulatory factors and their receptors, e.g. including ion channels}

Show less

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY