Detection of nucleic acids by multiple sequential invasive cleavages
Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
- Inventors:
- Issue Date:
- Research Org.:
- Third Wave Technologies, Inc. (Madison, WI)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1079451
- Patent Number(s):
- 8288093
- Application Number:
- 12/099,656
- Assignee:
- Third Wave Technologies, Inc. (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-94ER81891
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages. United States: N. p., 2012.
Web.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, & Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages. United States.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Tue .
"Detection of nucleic acids by multiple sequential invasive cleavages". United States. https://www.osti.gov/servlets/purl/1079451.
@article{osti_1079451,
title = {Detection of nucleic acids by multiple sequential invasive cleavages},
author = {Hall, Jeff G and Lyamichev, Victor I and Mast, Andrea L and Brow, Mary Ann D},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 16 00:00:00 EDT 2012},
month = {Tue Oct 16 00:00:00 EDT 2012}
}
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