Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system
Abstract
The present invention describes a process for saccharification of lignocelluloses to sugars using whole microbial cells, which are enriched from cultures inoculated with paper mill waste water, wood processing waste and soil. A three-member bacterial consortium is selected as a potent microbial inocula and immobilized on inedible plant fibers for biomass saccharification. The present invention further relates the design of a dual bioreactor system, with various biocarriers for enzyme immobilization and repeated use. Sugars are continuously removed eliminating end-product inhibition and consumption by cell.
- Inventors:
-
- Okemos, MI
- Montgomery, AL
- Issue Date:
- Research Org.:
- Technova Corp
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1039201
- Patent Number(s):
- 8143040
- Application Number:
- 12/379,569
- Assignee:
- Metna Co (Lansing, MI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- DOE Contract Number:
- FG02-07ER84872
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Lu, Jue, and Okeke, Benedict. Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system. United States: N. p., 2012.
Web.
Lu, Jue, & Okeke, Benedict. Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system. United States.
Lu, Jue, and Okeke, Benedict. Tue .
"Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system". United States. https://www.osti.gov/servlets/purl/1039201.
@article{osti_1039201,
title = {Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system},
author = {Lu, Jue and Okeke, Benedict},
abstractNote = {The present invention describes a process for saccharification of lignocelluloses to sugars using whole microbial cells, which are enriched from cultures inoculated with paper mill waste water, wood processing waste and soil. A three-member bacterial consortium is selected as a potent microbial inocula and immobilized on inedible plant fibers for biomass saccharification. The present invention further relates the design of a dual bioreactor system, with various biocarriers for enzyme immobilization and repeated use. Sugars are continuously removed eliminating end-product inhibition and consumption by cell.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 27 00:00:00 EDT 2012},
month = {Tue Mar 27 00:00:00 EDT 2012}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Cellulase families and their genes
journal, September 1987
- Knowles, Jonathan; Lehtovaara, Päivi; Teeri, Tuula
- Trends in Biotechnology, Vol. 5, Issue 9, p. 255-261
Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticollis
journal, January 2002
- Wenzel, M.; Schonig, I.; Berchtold, M.
- Journal of Applied Microbiology, Vol. 92, Issue 1
Two cellulases, CelA and CelC, from the polycentric anaerobic fungus Orpinomyces strain PC-2 contain N-terminal docking domains for a cellulase-hemicellulase complex.
journal, January 1997
- Li, X. L.; Chen, H.; Ljungdahl, L. G.
- Applied and environmental microbiology, Vol. 63, Issue 12
What is (and is not) vital to advancing cellulosic ethanol
journal, April 2007
- Wyman, Charles E.
- Trends in Biotechnology, Vol. 25, Issue 4
Microbial xylanolytic systems
journal, November 1985
- Biely, Peter
- Trends in Biotechnology, Vol. 3, Issue 11
Investing in green and white biotech
journal, July 2006
- Bevan, Michael W.; Franssen, Maurice C. R.
- Nature Biotechnology, Vol. 24, Issue 7