Hydroxyapatite-binding peptides for bone growth and inhibition
Abstract
Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp).sub.x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals .about.40 nm in size.
- Inventors:
-
- Berkeley, CA
- Shrewsbury, MA
- Walnut Creek, CA
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1027207
- Patent Number(s):
- 8022040
- Application Number:
- 11/720,427
- Assignee:
- LBNL
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61L - METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL
- DOE Contract Number:
- AC03-76SF00098; AC02-05CH11231
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Bertozzi, Carolyn R, Song, Jie, and Lee, Seung-Wuk. Hydroxyapatite-binding peptides for bone growth and inhibition. United States: N. p., 2011.
Web.
Bertozzi, Carolyn R, Song, Jie, & Lee, Seung-Wuk. Hydroxyapatite-binding peptides for bone growth and inhibition. United States.
Bertozzi, Carolyn R, Song, Jie, and Lee, Seung-Wuk. Tue .
"Hydroxyapatite-binding peptides for bone growth and inhibition". United States. https://www.osti.gov/servlets/purl/1027207.
@article{osti_1027207,
title = {Hydroxyapatite-binding peptides for bone growth and inhibition},
author = {Bertozzi, Carolyn R and Song, Jie and Lee, Seung-Wuk},
abstractNote = {Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp).sub.x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals .about.40 nm in size.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 20 00:00:00 EDT 2011},
month = {Tue Sep 20 00:00:00 EDT 2011}
}
Works referenced in this record:
Crystal and molecular structure of a collagen-like peptide at 1.9 A resolution
journal, October 1994
- Bella, J.; Eaton, M.; Brodsky, B.
- Science, Vol. 266, Issue 5182
Osteopontin Facilitates Bone Resorption, Decreasing Bone Mineral Crystallinity and Content During Calcium Deficiency
journal, July 2003
- Shapses, S. A.; Cifuentes, M.; Spevak, L.
- Calcified Tissue International, Vol. 73, Issue 1
Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1
journal, July 2003
- He, Gen; Dahl, Tom; Veis, Arthur
- Nature Materials, Vol. 2, Issue 8
Regulating Bone Growth and Development with Bone Morphogenetic Proteins
journal, April 2006
- Leboy, P. S.
- Annals of the New York Academy of Sciences, Vol. 1068, Issue 1
Design strategies in mineralized biological materials
journal, January 1997
- Weiner, Stephen; Addadi, Lia
- Journal of Materials Chemistry, Vol. 7, Issue 5
M13 bacteriophage displaying disulfide-constrained microproteins
journal, June 1993
- McLafferty, M. A.; Kent, R. B.; Ladner, R. C.
- Gene, Vol. 128, Issue 1
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997
- Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
- Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
Control and Design Principles in Biological Mineralization
journal, February 1992
- Addadi, Lia; Weiner, Stephen
- Angewandte Chemie International Edition in English, Vol. 31, Issue 2
Hand2 regulates chondrogenesis in vitro and in vivo
journal, May 2010
- Abe, Makoto; Michikami, Ikumi; Fukushi, Toshiya
- Bone, Vol. 46, Issue 5
Ordering of Quantum Dots Using Genetically Engineered Viruses
journal, May 2002
- Lee, S. -W.
- Science, Vol. 296, Issue 5569
Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly
journal, June 2000
- Whaley, Sandra R.; English, D. S.; Hu, Evelyn L.
- Nature, Vol. 405, Issue 6787
Solid-phase peptide synthesis: a silver anniversary report*
journal, December 1987
- Barany, George; Kneib-Cordonier, Nancy; Mullen, Daniel G.
- International Journal of Peptide and Protein Research, Vol. 30, Issue 6
Convergent solid-phase peptide synthesis
journal, January 1993
- Lloyd-Williams, Paul; Albericio, Fernando; Giralt, Ernest
- Tetrahedron, Vol. 49, Issue 48
Virus-Based Toolkit for the Directed Synthesis of Magnetic and Semiconducting Nanowires
journal, January 2004
- Mao, C.
- Science, Vol. 303, Issue 5655
Skeletal Overexpression of Connective Tissue Growth Factor Impairs Bone Formation and Causes Osteopenia
journal, September 2008
- Smerdel-Ramoya, Anna; Zanotti, Stefano; Stadmeyer, Lisa
- Endocrinology, Vol. 149, Issue 9
Delineation of the Hydroxyapatite-nucleating Domains of Bone Sialoprotein
journal, December 2002
- Tye, Coralee E.; Rattray, Kevin R.; Warner, Kevin J.
- Journal of Biological Chemistry, Vol. 278, Issue 10
The Carboxyl-terminal Domain of Phosphophoryn Contains Unique Extended Triplet Amino Acid Repeat Sequences Forming Ordered Carboxyl-Phosphate Interaction Ridges That May Be Essential in the Biomineralization Process
journal, December 1996
- George, Anne; Bannon, Leslie; Sabsay, Boris
- Journal of Biological Chemistry, Vol. 271, Issue 51
Nephroblastoma Overexpressed (Nov) Is a Novel Bone Morphogenetic Protein Antagonist
journal, November 2007
- Canalis, E.
- Annals of the New York Academy of Sciences, Vol. 1116, Issue 1
Biological Routes to Metal Alloy Ferromagnetic Nanostructures
journal, June 2004
- Reiss, Brian D.; Mao, Chuanbin; Solis, Daniel J.
- Nano Letters, Vol. 4, Issue 6
Self-Assembly and Mineralization of Peptide-Amphiphile Nanofibers
journal, November 2001
- Hartgerink, Jeffrey D.; Beniash, Elia; Stupp, Samuel I.
- Science, Vol. 294, Issue 5547, p. 1684-1688
Random peptide libraries: a source of specific protein binding molecules
journal, July 1990
- Devlin, J.; Panganiban, L.; Devlin, P.
- Science, Vol. 249, Issue 4967
Searching for peptide ligands with an epitope library
journal, July 1990
- Scott, J.; Smith, G.
- Science, Vol. 249, Issue 4967
Modulation of crystal formation by bone phosphoproteins: role of glutamic acid-rich sequences in the nucleation of hydroxyapatite by bone sialoprotein
journal, August 1994
- Hunter, G. K.; Goldberg, H. A.
- Biochemical Journal, Vol. 302, Issue 1
Bacteriophage Display and Discovery of Peptide Leads for drug Development
journal, June 1997
- Lowman, H. B.
- Annual Review of Biophysics and Biomolecular Structure, Vol. 26, Issue 1