Photoswitchable method for the ordered attachment of proteins to surfaces
Abstract
Described herein is a method for the attachment of proteins to any solid support with control over the orientation of the attachment. The method is extremely efficient, not requiring the previous purification of the protein to be attached, and can be activated by UV-light. Spatially addressable arrays of multiple protein components can be generated by using standard photolithographic techniques.
- Inventors:
-
- Livermore, CA
- Clayton, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1019569
- Patent Number(s):
- 7972827
- Application Number:
- US Patent Application 12/708,382
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Camarero, Julio A, DeYoreo, James J, and Kwon, Youngeun. Photoswitchable method for the ordered attachment of proteins to surfaces. United States: N. p., 2011.
Web.
Camarero, Julio A, DeYoreo, James J, & Kwon, Youngeun. Photoswitchable method for the ordered attachment of proteins to surfaces. United States.
Camarero, Julio A, DeYoreo, James J, and Kwon, Youngeun. Tue .
"Photoswitchable method for the ordered attachment of proteins to surfaces". United States. https://www.osti.gov/servlets/purl/1019569.
@article{osti_1019569,
title = {Photoswitchable method for the ordered attachment of proteins to surfaces},
author = {Camarero, Julio A and DeYoreo, James J and Kwon, Youngeun},
abstractNote = {Described herein is a method for the attachment of proteins to any solid support with control over the orientation of the attachment. The method is extremely efficient, not requiring the previous purification of the protein to be attached, and can be activated by UV-light. Spatially addressable arrays of multiple protein components can be generated by using standard photolithographic techniques.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 05 00:00:00 EDT 2011},
month = {Tue Jul 05 00:00:00 EDT 2011}
}
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Works referenced in this record:
Activation of Protein Splicing by Protease- or Light-Triggered O to N Acyl Migration
journal, September 2008
- Vila-Perelló, Miquel; Hori, Yuichiro; Ribó, Marc
- Angewandte Chemie International Edition, Vol. 47, Issue 40
Characterization of a Naturally Occurring Trans-Splicing Intein from Synechocystis sp. PCC6803
journal, February 2001
- Martin, Deana D.; Xu, Ming-Qun; Evans, Thomas C.
- Biochemistry, Vol. 40, Issue 5
Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing
journal, May 2005
- Girish, Aparna; Sun, Hongyan; Yeo, Dawn S. Y.
- Bioorganic & Medicinal Chemistry Letters, Vol. 15, Issue 10
From combinatorial chemistry to chemical microarray
journal, June 2002
- Lam, Kit S.; Renil, Manat
- Current Opinion in Chemical Biology, Vol. 6, Issue 3
Local Photorelease of Caged Thymosin β4 in Locomoting Keratocytes Causes Cell Turning
journal, May 2001
- Roy, Partha; Rajfur, Zenon; Jones, David
- The Journal of Cell Biology, Vol. 153, Issue 5
Protein patterning
journal, April 1998
- Blawas, A. S.; Reichert, W. M.
- Biomaterials, Vol. 19, Issue 7-9
Protein trans -Splicing and Cyclization by a Naturally Split Intein from the dnaE Gene of Synechocystis Species PCC6803
journal, March 2000
- Evans, Thomas C.; Martin, Deana; Kolly, Reto
- Journal of Biological Chemistry, Vol. 275, Issue 13
Editorial [Hot Topic: Polypeptide Chemical Ligation Tools in Protein Engineering (Guest Editor: Julio A. Camarero)]
journal, November 2005
- Camarero, Julio
- Protein & Peptide Letters, Vol. 12, Issue 8
Synthesis of Proteins by Native Chemical Ligation Using Fmoc-Based Chemistry
journal, November 2005
- Camarero, Julio; Mitchell, Alexander
- Protein & Peptide Letters, Vol. 12, Issue 8
Chemoselective Attachment of Biologically Active Proteins to Surfaces by Expressed Protein Ligation and Its Application for “Protein Chip” Fabrication
journal, November 2004
- Camarero, Julio A.; Kwon, Youngeun; Coleman, Matthew A.
- Journal of the American Chemical Society, Vol. 126, Issue 45, p. 14730-14731
Protein purification via temperature-dependent, intein-mediated cleavage from an immobilized metal affinity resin
journal, September 2006
- Mills, Kenneth V.; Connor, Katherine R.; Dorval, Deirdre M.
- Analytical Biochemistry, Vol. 356, Issue 1
Protein trans -splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803
journal, August 1998
- Wu, Hong; Hu, Zhuma; Liu, Xiang-Qin
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 16
Photomodulation of Protein Trans-Splicing Through Backbone Photocaging of the DnaE Split Intein
journal, May 2010
- Berrade, Luis; Kwon , Youngeun; Camarero, Julio A.
- ChemBioChem, Vol. 11, Issue 10
New Developments for the Site-Specific Attachment of Protein to Surfaces
journal, January 2006
- Camarero, Julio A.
- Biophysical Reviews and Letters, Vol. 01, Issue 01
Membrane Protein Microarrays
journal, February 2002
- Fang, Ye; Frutos, Anthony G.; Lahiri, Joydeep
- Journal of the American Chemical Society, Vol. 124, Issue 11, p. 2394-2395
Selective Immobilization of Proteins onto Solid Supports through Split-Intein-Mediated Protein Trans-Splicing
journal, March 2006
- Kwon, Youngeun; Coleman, Matthew A.; Camarero, Julio A.
- Angewandte Chemie International Edition, Vol. 45, Issue 11