Title: Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate

Abstract

Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.

Inventors:

Caimi, Perry G ^[1]; Chou, Yat-Chen ^[2]; Franden, Mary Ann ^[3]; Knoke, Kyle ^[4]; Tao, Luan ^[5]; Viitanen, Paul V ^[6]; Zhang, Min ^[2]; Zhang, Yuying ^[7]

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+ Show Inventor Affiliations

1. Kennett Square, PA
2. Lakewood, CO
3. Centennial, CO
4. Newark, DE
5. Havertown, PA
6. West Chester, PA
7. New Hope, PA

Issue Date:

Tue Mar 01 00:00:00 EST 2011

Research Org.:

National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1016555

Patent Number(s):

7897396

Application Number:

US Patent Application 12/261,160

Assignee:

E.I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

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C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/065 - {with microorganisms other than yeasts}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

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DOE Contract Number:  

AC36-08GO28308

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

60 APPLIED LIFE SCIENCES