Neisseria meningitidis serogroup A capsular polysaccharide acetyltransferase, methods and compositions

Stephens, David S; Gudlavalleti, Seshu K; Tzeng, Yih-Ling; Datta, Anup K; Carlson, Russell W

Title: Neisseria meningitidis serogroup A capsular polysaccharide acetyltransferase, methods and compositions

Abstract

Provided are methods for recombinant production of an O-acetyltransferase and methods for acetylating capsular polysaccharides, especially those of a Serogroup A Neisseria meningitidis using the recombinant O-acetyltransferase, and immunogenic compositions comprising the acetylated capsular polysaccharide.

Inventors:

Stephens, David S ^[1]; Gudlavalleti, Seshu K ^[2]; Tzeng, Yih-Ling ^[3]; Datta, Anup K ^[4]; Carlson, Russell W ^[5]

Stone Mountain, GA
Kensington, MD
Atlanta, GA
San Diego, CA
Athens, GA

Issue Date:: Tue Feb 08 00:00:00 EST 2011

Research Org.:: Stephens, David S. (Stone Mountain, GA); Gudlavalleti, Seshu K. (Kensington, MD); Tzeng, Yih-Ling (Atlanta, GA); Datta, Anup K. (San Diego, CA); Carlson, Russell W. (Athens, GA)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1015405

Patent Number(s):: 7883876

Application Number:: US Patent Application 11/943,326

Assignee:: Stephens, David S. (Stone Mountain, GA); Gudlavalleti, Seshu K. (Kensington, MD); Tzeng, Yih-Ling (Atlanta, GA); Datta, Anup K. (San Diego, CA); Carlson, Russell W. (Athens, GA)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

Show more

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A61K39/00 - Medicinal preparations containing antigens or antibodies
A61K39/095 - Neisseria

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A61P31/04 - Antibacterial agents

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/1029 - {transferring groups other than amino-acyl groups

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/44 - Preparation of O-glycosides, e.g. glucosides {

Show less

DOE Contract Number:: FG02-93ER20097

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Stephens, David S, Gudlavalleti, Seshu K, Tzeng, Yih-Ling, Datta, Anup K, and Carlson, Russell W. Neisseria meningitidis serogroup A capsular polysaccharide acetyltransferase, methods and compositions.  United States: N. p., 2011. 
        Web.

Copy to clipboard


                    Stephens, David S, Gudlavalleti, Seshu K, Tzeng, Yih-Ling, Datta, Anup K, & Carlson, Russell W. Neisseria meningitidis serogroup A capsular polysaccharide acetyltransferase, methods and compositions.  United States.

Copy to clipboard


                    Stephens, David S, Gudlavalleti, Seshu K, Tzeng, Yih-Ling, Datta, Anup K, and Carlson, Russell W. Tue .  
        "Neisseria meningitidis serogroup A capsular polysaccharide acetyltransferase, methods and compositions".  United States.  https://www.osti.gov/servlets/purl/1015405.

Copy to clipboard


                    
@article{osti_1015405,

  title        = {Neisseria meningitidis serogroup A capsular polysaccharide acetyltransferase, methods and compositions},

  author       = {Stephens, David S and Gudlavalleti, Seshu K and Tzeng, Yih-Ling and Datta, Anup K and Carlson, Russell W},

  abstractNote = {Provided are methods for recombinant production of an O-acetyltransferase and methods for acetylating capsular polysaccharides, especially those of a Serogroup A Neisseria meningitidis using the recombinant O-acetyltransferase, and immunogenic compositions comprising the acetylated capsular polysaccharide.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Feb 08 00:00:00 EST 2011},

  month        = {Tue Feb 08 00:00:00 EST 2011}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Possible dissociation of the heparin-binding and mitogenic activities of heparin-binding (acidic fibroblast) growth factor-1 from its receptor-binding activities by site-directed mutagenesis of a single lysine residue.
journal, November 1990

Burgess, W. H.; Shaheen, A. M.; Ravera, M.
The Journal of Cell Biology, Vol. 111, Issue 5
https://doi.org/10.1083/jcb.111.5.2129

Molecular characterization of the O-acetyl transferase gene of converting bacteriophage SF6 that adds group antigen 6 to Shigella flexneri
journal, January 1991

Verma, N. K.; Brandt, J. M.; Verma, D. J.
Molecular Microbiology, Vol. 5, Issue 1
https://doi.org/10.1111/j.1365-2958.1991.tb01827.x

Structure, assembly and regulation of expression of capsules in Escherichia coli
journal, March 1999

Whitfield, Chris; Roberts, Ian S.
Molecular Microbiology, Vol. 31, Issue 5
https://doi.org/10.1046/j.1365-2958.1999.01276.x

Nodulation protein NodL of Rhizobium leguminosarum O-acetylates lipo-oligosaccharides, chitin fragments and N-acetylglucosamine in vitro
journal, February 1994

Bloemberg, Guido V.; Thomas-Oates, Jane E.; Lugtenberg, Ben J. J.
Molecular Microbiology, Vol. 11, Issue 4
https://doi.org/10.1111/j.1365-2958.1994.tb00357.x

Characterization of epidemic Neisseria meningitidis serogroup C strains in several Brazilian states.
journal, January 1994

Sacchi, C. T.; Tondella, M. L.; de Lemos, A. P.
Journal of Clinical Microbiology, Vol. 32, Issue 7
https://doi.org/10.1128/JCM.32.7.1783-1787.1994

The Changing Epidemiology of Invasive Meningococcal Disease in Canada, 1985 Through 1992: Emergence of a Virulent Clone of Neisseria meningitidis
journal, February 1995

Whalen, Christine M.
JAMA, Vol. 273, Issue 5
https://doi.org/10.1001/jama.1995.03520290042027

Relation between structure and immunologic properties of the Vi capsular polysaccharide.
journal, January 1991

Szu, S. C.; Li, X. R.; Stone, A. L.
Infection and Immunity, Vol. 59, Issue 12
https://doi.org/10.1128/IAI.59.12.4555-4561.1991

Identification of a gene essential for O-acetylation of the Staphylococcus aureus type 5 capsular polysaccharide
journal, January 1998

Bhasin, Navneet; Albus, Anne; Michon, Francis
Molecular Microbiology, Vol. 27, Issue 1
https://doi.org/10.1046/j.1365-2958.1998.00646.x

The Biochemistry and Genetics of Capsular Polysaccharide Production in Bacteria
journal, October 1996

Roberts, Ian S.
Annual Review of Microbiology, Vol. 50, Issue 1
https://doi.org/10.1146/annurev.micro.50.1.285

Powers and Pitfalls in Sequence Analysis: The 70% Hurdle
journal, April 2000

Bork, P.
Genome Research, Vol. 10, Issue 4, p. 398-400
https://doi.org/10.1101/gr.10.4.398

Safety and immunogenicity of a new Neisseria meningitidis serogroup C-tetanus toxoid conjugate vaccine in healthy adults
journal, November 1999

Richmond, Peter; Goldblatt, David; Fusco, Peter C.
Vaccine, Vol. 18, Issue 7-8
https://doi.org/10.1016/S0264-410X(99)00276-5

Epidemic Meningococcal Disease in Nairobi, Kenya, 1989
journal, August 1992

Pinner, R. W.; Onyango, F.; Perkins, B. A.
Journal of Infectious Diseases, Vol. 166, Issue 2
https://doi.org/10.1093/infdis/166.2.359

Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes.
journal, March 1990

Karlin, S.; Altschul, S. F.
Proceedings of the National Academy of Sciences, Vol. 87, Issue 6
https://doi.org/10.1073/pnas.87.6.2264

Naturally-acquired immunity to Neisseria meningitidis group A
journal, January 2005

Amir, Jacob; Louie, Lesile; Granoff, Dan M.
Vaccine, Vol. 23, Issue 8
https://doi.org/10.1016/j.vaccine.2004.07.042

Genetic and biochemical evidence of a Campylobacter jejuni capsular polysaccharide that accounts for Penner serotype specificity: Genetics and biochemistry of C. jejuni LPS biosynthesis
journal, February 2000

Karlyshev, Andrey V.; Linton, Dennis; Gregson, Norman A.
Molecular Microbiology, Vol. 35, Issue 3
https://doi.org/10.1046/j.1365-2958.2000.01717.x

Genetics of capsule O-acetylation in serogroup C, W-135 and Y meningococci: Capsule O-acetylation in meningococci
journal, November 2003

Claus, Heike; Borrow, Ray; Achtman, Mark
Molecular Microbiology, Vol. 51, Issue 1
https://doi.org/10.1046/j.1365-2958.2003.03819.x

Antigenic Determinants of Staphylococcus aureus Type 5 and Type 8 Capsular Polysaccharide Vaccines
journal, October 1998

Fattom, Ali I.; Sarwar, Jawad; Basham, Lisa
Infection and Immunity, Vol. 66, Issue 10
https://doi.org/10.1128/IAI.66.10.4588-4592.1998

Full 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharides from Neisseria meningitidis used in vaccine production
journal, December 1996

Lemercinier, Xavier; Jones, Christopher
Carbohydrate Research, Vol. 296, Issue 1-4
https://doi.org/10.1016/S0008-6215(96)00253-4

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
https://doi.org/10.1093/nar/25.17.3389

INFECTIOUS DISEASE: An Ill Wind, Bringing Meningitis
journal, June 2008

Roberts, L.
Science, Vol. 320, Issue 5884
https://doi.org/10.1126/science.320.5884.1710

Structural and Mechanistic Studies of Galactoside Acetyltransferase, the Escherichia coli LacA Gene Product
journal, November 1995

Lewendon, Ann; Ellis, Jacqueline; Shaw, William V.
Journal of Biological Chemistry, Vol. 270, Issue 44
https://doi.org/10.1074/jbc.270.44.26326

Endotoxin of Neisseria meningitidis Composed Only of Intact Lipid A: Inactivation of the Meningococcal 3-Deoxy-d-Manno-Octulosonic Acid Transferase
journal, May 2002

Tzeng, Yih-Ling; Datta, Anup; Kolli, V. Kumar
Journal of Bacteriology, Vol. 184, Issue 9
https://doi.org/10.1128/JB.184.9.2379-2388.2002

Kinetic and hydrodynamic studies of the NodL O-acetyl transferase of Rhizobium leguminosarum: a random-order ternary complex mechanism for acetyl transfer by a roughly spherical trimeric protein
journal, June 2000

Hindson, V. John; Dunn, Stephan O.; Rowe, Arthur J.
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1479, Issue 1-2
https://doi.org/10.1016/S0167-4838(00)00039-X

O -Acetylation status of the capsular polysaccharides of serogroup Y and W135 meningococci isolated in the UK
journal, January 2002

Longworth, Emma; Fernsten, Phil; Mininni, Terri L.
FEMS Immunology & Medical Microbiology, Vol. 32, Issue 2
https://doi.org/10.1111/j.1574-695X.2002.tb00543.x

Host Recognition of Bacterial Muramyl Dipeptide Mediated through NOD2: IMPLICATIONS FOR CROHN′S DISEASE
journal, January 2003

Inohara, Naohiro; Ogura, Yasunori; Fontalba, Ana
Journal of Biological Chemistry, Vol. 278, Issue 8
https://doi.org/10.1074/jbc.C200673200

Colorimetric Method for Determination of Sugars and Related Substances
journal, March 1956

DuBois, Michel.; Gilles, K. A.; Hamilton, J. K.
Analytical Chemistry, Vol. 28, Issue 3, p. 350-356
https://doi.org/10.1021/ac60111a017

Interactions of VirB9, -10, and -11 with the membrane fraction of Agrobacterium tumefaciens: solubility studies provide evidence for tight associations.
journal, January 1995

Finberg, K. E.; Muth, T. R.; Young, S. P.
Journal of bacteriology, Vol. 177, Issue 17
https://doi.org/10.1128/JB.177.17.4881-4889.1995

Antibody Responses to Capsular Polysaccharide Backbone and O-Acetate Side Groups of Streptococcus pneumoniae Type 9V in Humans and Rhesus Macaques
journal, August 1998

McNeely, Tessie B.; Staub, Joan M.; Rusk, Cynthia M.
Infection and Immunity, Vol. 66, Issue 8
https://doi.org/10.1128/IAI.66.8.3705-3710.1998

Resistance to nodulation of cv. Afghanistan peas is overcome by nodX , which mediates an O-acetylation of the Rhizobium leguminosarum lipo-oligosaccharide nodulation factor
journal, October 1993

Firmin, J. L.; Wilson, K. E.; Carlson, R. W.
Molecular Microbiology, Vol. 10, Issue 2
https://doi.org/10.1111/j.1365-2958.1993.tb01961.x

Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile "Caldocellum saccharolyticum".
journal, January 1990

Lüthi, E.; Love, D. R.; McAnulty, J.
Applied and Environmental Microbiology, Vol. 56, Issue 4
https://doi.org/10.1128/AEM.56.4.1017-1024.1990

Structures of the Capsular Polysaccharides of Neisseria meningitidis as Determined by 13C-Nuclear Magnetic Resonance Spectroscopy
journal, August 1977

Jennings, H. J.; Bhauacharjee, A. K.; Bundle, D. R.
Journal of Infectious Diseases, Vol. 136, Issue Supplement 1
https://doi.org/10.1093/infdis/136.Supplement.S78

Genetic Diversity of Penicillin-Resistant Neisseria meningitidis
journal, July 1992

Campos, J.; Fuste, M. C.; Trujillo, G.
Journal of Infectious Diseases, Vol. 166, Issue 1
https://doi.org/10.1093/infdis/166.1.173

Human Immunity to the Meningococcus
journal, June 1969

Gotschlich, Emil C.; Liu, Teh Yung; Artenstein, Malcolm S.
The Journal of Experimental Medicine, Vol. 129, Issue 6
https://doi.org/10.1084/jem.129.6.1349

The serine acetyltransferase from Escherichia coli: Over-expression, purification and preliminary crystallographic analysis
journal, December 1990

Wigley, Dale B.; Derrick, Jeremy P.; Shaw, William V.
FEBS Letters, Vol. 277, Issue 1-2
https://doi.org/10.1016/0014-5793(90)80862-D

Evaluation of De-O-Acetylated Meningococcal C Polysaccharide-Tetanus Toxoid Conjugate Vaccine in Infancy: Reactogenicity, Immunogenicity, Immunologic Priming, and Bactericidal Activity against O-Acetylated and De-O-Acetylated Serogroup C Strains
journal, April 2001

Richmond, Peter; Borrow, Ray; Findlow, Jamie
Infection and Immunity, Vol. 69, Issue 4
https://doi.org/10.1128/IAI.69.4.2378-2382.2001

Risk of group A meningococcal disease: bacterial interference and cross-reactive bacteria among mucosal flora.
journal, January 1985

Filice, G. A.; Hayes, P. S.; Counts, G. W.
Journal of Clinical Microbiology, Vol. 22, Issue 2
https://doi.org/10.1128/JCM.22.2.152-156.1985

Development and Evaluation of an Improved Mouse Model of Meningococcal Colonization
journal, April 2003

Yi, Kyungcheol; Stephens, David S.; Stojiljkovic, Igor
Infection and Immunity, Vol. 71, Issue 4
https://doi.org/10.1128/IAI.71.4.1849-1855.2003

Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
journal, January 1981

Beaucage, S. L.; Caruthers, M. H.
Tetrahedron Letters, Vol. 22, Issue 20, p. 1859-1862
https://doi.org/10.1016/S0040-4039(01)90461-7

Studies on transformation of Escherichia coli with plasmids
journal, June 1983

Hanahan, Douglas
Journal of Molecular Biology, Vol. 166, Issue 4
https://doi.org/10.1016/S0022-2836(83)80284-8

A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module: Modular cinnamoyl esterase from P. funiculosum
journal, December 2000

Kroon, Paul A.; Williamson, Gary; Fish, Neville M.
European Journal of Biochemistry, Vol. 267, Issue 23
https://doi.org/10.1046/j.1432-1033.2000.01742.x

Serogroup C meningococcal outbreaks in the United States. An emerging threat
journal, February 1995

Jackson, L. A.
JAMA: The Journal of the American Medical Association, Vol. 273, Issue 5
https://doi.org/10.1001/jama.273.5.383

Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide.
journal, March 1979

Orskov, F.; Orskov, I.; Sutton, A.
The Journal of Experimental Medicine, Vol. 149, Issue 3
https://doi.org/10.1084/jem.149.3.669

Meningococcal vaccines—present and future
journal, January 1991

Zollinger, Wendell D.; Moran, Ellen
Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 85
https://doi.org/10.1016/0035-9203(91)90339-Z

Basic local alignment search tool
journal, October 1990

Altschul, Stephen F.; Gish, Warren; Miller, Webb
Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
https://doi.org/10.1016/S0022-2836(05)80360-2

Use and validation of NMR assays for the identity and O-acetyl content of capsular polysaccharides from Neisseria meningitidis used in vaccine manufacture
journal, November 2002

Jones, Christopher; Lemercinier, Xavier
Journal of Pharmaceutical and Biomedical Analysis, Vol. 30, Issue 4
https://doi.org/10.1016/S0731-7085(02)00462-4

Effect of O Acetylation of Neisseria meningitidis Serogroup A Capsular Polysaccharide on Development of Functional Immune Responses
journal, July 2002

Berry, David S.; Lynn, Freyja; Lee, Che-Hung
Infection and Immunity, Vol. 70, Issue 7
https://doi.org/10.1128/IAI.70.7.3707-3713.2002

The (α2→8)-Linked Polysialic Acid Capsule and Lipooligosaccharide Structure Both Contribute to the Ability of Serogroup B Neisseria meningitidis To Resist the Bactericidal Activity of Normal Human Serum
journal, December 1998

Kahler, C. M.; Martin, L. E.; Shih, G. C.
Infection and Immunity, Vol. 66, Issue 12
https://doi.org/10.1128/IAI.66.12.5939-5947.1998

A point mutation in the active site of O-acetyltransferase results in modified lipopolysaccharide but does not influence virulence
journal, January 2001

Luck, P.
International Journal of Medical Microbiology, Vol. 291, Issue 5
https://doi.org/10.1078/1438-4221-00140

Mutant Analysis and Cellular Localization of the AlgI, AlgJ, and AlgF Proteins Required for O Acetylation of Alginate in Pseudomonas aeruginosa
journal, June 2002

Franklin, Michael J.; Ohman, Dennis E.
Journal of Bacteriology, Vol. 184, Issue 11
https://doi.org/10.1128/JB.184.11.3000-3007.2002

The human oestrogen receptor functions in yeast
journal, July 1988

Metzger, D.; White, J. H.; Chambon, P.
Nature, Vol. 334, Issue 6177
https://doi.org/10.1038/334031a0

Synthesis of deoxyoligonucleotides on a polymer support
journal, June 1981

Matteucci, M. D.; Caruthers, M. H.
Journal of the American Chemical Society, Vol. 103, Issue 11
https://doi.org/10.1021/ja00401a041

Human bactericidal antibody response to meningococcal outer membrane protein vaccines
journal, January 1987

Zollinger, W. D.; Boslego, J.; Fr�holm, L. O.
Antonie van Leeuwenhoek, Vol. 53, Issue 6
https://doi.org/10.1007/BF00415494

Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus.
journal, January 1997

Sau, S.; Sun, J.; Lee, C. Y.
Journal of bacteriology, Vol. 179, Issue 5
https://doi.org/10.1128/JB.179.5.1614-1621.1997

Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern.
journal, January 1987

Caugant, D. A.; Mocca, L. F.; Frasch, C. E.
Journal of Bacteriology, Vol. 169, Issue 6
https://doi.org/10.1128/JB.169.6.2781-2792.1987

Complement Activation and Formation of the Membrane Attack Complex on Serogroup B Neisseria meningitidis in the Presence or Absence of Serum Bactericidal Activity
journal, July 2002

Drogari-Apiranthitou, M.; Kuijper, E. J.; Dekker, N.
Infection and Immunity, Vol. 70, Issue 7
https://doi.org/10.1128/IAI.70.7.3752-3758.2002

Structural identification of the iipo-chitin oligosaccharide nodulation signals of Rhizobium loti
journal, February 1995

López-Lara, Isabel M.; Berg, Jorrit D. J.; Thomas-Oates, Jane E.
Molecular Microbiology, Vol. 15, Issue 4
https://doi.org/10.1111/j.1365-2958.1995.tb02372.x

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
journal, January 1985

Yanisch-Perron, Celeste; Vieira, Jeffrey; Messing, Joachim
Gene, Vol. 33, Issue 1
https://doi.org/10.1016/0378-1119(85)90120-9

Directed Mutagenesis
journal, December 1981

Shortle, D.; DiMaio, D.; Nathans, D.
Annual Review of Genetics, Vol. 15, Issue 1
https://doi.org/10.1146/annurev.ge.15.120181.001405

Interaction of Neisseria meningitidis with Human Nasopharyngeal Mucosa: Attachment and Entry into Columnar Epithelial Cells
journal, September 1983

Stephens, D. S.; Hoffman, L. H.; McGee, Z. A.
Journal of Infectious Diseases, Vol. 148, Issue 3
https://doi.org/10.1093/infdis/148.3.369

Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988

Lazar, E.; Watanabe, S.; Dalton, S.
Molecular and Cellular Biology, Vol. 8, Issue 3, p. 1247-1252
https://doi.org/10.1128/MCB.8.3.1247

Neisseria meningitidis Strains Isolated from Invasive Infections in France (1999-2002): Phenotypes and Antibiotic Susceptibility Patterns
journal, October 2003

Antignac, A.; Ducos-Galand, M.; Guiyoule, A.
Clinical Infectious Diseases, Vol. 37, Issue 7
https://doi.org/10.1086/377739

Rhizobium fredii and Rhizobium meliloti produce 3-deoxy-D-manno-2-octulosonic acid-containing polysaccharides that are structurally analogous to group II K antigens (capsular polysaccharides) found in Escherichia coli.
journal, January 1993

Reuhs, B. L.; Carlson, R. W.; Kim, J. S.
Journal of Bacteriology, Vol. 175, Issue 11
https://doi.org/10.1128/JB.175.11.3570-3580.1993

Hitting Early, Epidemic Meningitis Ravages Nigeria and Niger
journal, April 2009

Roberts, Leslie
Science, Vol. 324, Issue 5923
https://doi.org/10.1126/science.324.5923.20

A macrolide 3-O-acyltransferase gene from the midecamycin-producing species Streptomyces mycarofaciens.
journal, January 1992

Hara, O.; Hutchinson, C. R.
Journal of Bacteriology, Vol. 174, Issue 15
https://doi.org/10.1128/JB.174.15.5141-5144.1992

A novel highly unsaturated fatty acid moiety of lipo-oligosaccharide signals determines host specificity of Rhizobium
journal, November 1991

Spaink, Herman P.; Sheeley, Douglas M.; van Brussel, Anton A. N.
Nature, Vol. 354, Issue 6349
https://doi.org/10.1038/354125a0

Isolating "Uncultivable" Microorganisms in Pure Culture in a Simulated Natural Environment
journal, May 2002

Kaeberlein, T.
Science, Vol. 296, Issue 5570
https://doi.org/10.1126/science.1070633

L-Cysteine Biosynthesis in Escherichia coli: Nucleotide Sequence and Expression of the Serine Acetyltransferase (cysE) Gene from the Wild-type and a Cysteine-excreting Mutant
journal, March 1987

Denk, D.; Bock, A.
Microbiology, Vol. 133, Issue 3
https://doi.org/10.1099/00221287-133-3-515

The Neisseria meningitidis Serogroup A Capsular Polysaccharide O-3 and O-4 Acetyltransferase
journal, August 2004

Gudlavalleti, Seshu K.; Datta, Anup K.; Tzeng, Yih-Ling
Journal of Biological Chemistry, Vol. 279, Issue 41
https://doi.org/10.1074/jbc.M313552200

Engineering proteins for purification
journal, January 1990

Sassenfeld, H.
Trends in Biotechnology, Vol. 8
https://doi.org/10.1016/0167-7799(90)90145-N

Induction and repression of outer membrane proteins by anaerobic growth of Neisseria gonorrhoeae.
journal, January 1987

Clark, V. L.; Campbell, L. A.; Palermo, D. A.
Infection and Immunity, Vol. 55, Issue 6
https://doi.org/10.1128/IAI.55.6.1359-1364.1987

Detection of Conserved N -Linked Glycans and Phase-variable Lipooligosaccharides and Capsules from Campylobacter Cells by Mass Spectrometry and High Resolution Magic Angle Spinning NMR Spectroscopy
journal, April 2003

Szymanski, Christine M.; Michael, Frank St.; Jarrell, Harold C.
Journal of Biological Chemistry, Vol. 278, Issue 27
https://doi.org/10.1074/jbc.M301273200

Practical Methods in Molecular Biology
book, January 1981

Schleif, Robert F.; Wensink, Pieter C.
https://doi.org/10.1007/978-1-4612-5956-5

Deciphering the message in protein sequences: tolerance to amino acid substitutions
journal, March 1990

Bowie, J.; Reidhaar-Olson, J.; Lim, W.
Science, Vol. 247, Issue 4948, p. 1306-1310
https://doi.org/10.1126/science.2315699

Structural determination of the polysaccharide antigens of Neisseria meningitidis serogroups Y, W-135, and BO
journal, January 1976

Bhattacharjee, Apurba K.; Jennings, Harold J.; Kenny, C. Paul
Canadian Journal of Biochemistry, Vol. 54, Issue 1
https://doi.org/10.1139/o76-001

Characterization of the Gene Cassette Required for Biosynthesis of the (α1→6)-LinkedN-Acetyl-d-Mannosamine-1-Phosphate Capsule of Serogroup A Neisseria meningitidis
journal, March 1998

Swartley, John S.; Liu, Li-Jun; Miller, Yoon K.
Journal of Bacteriology, Vol. 180, Issue 6
https://doi.org/10.1128/JB.180.6.1533-1539.1998

Chemical methods for the analysis of sulphated galactans from red algae
journal, March 1991

Stevenson, Thomas T.; Furneaux, Richard H.
Carbohydrate Research, Vol. 210
https://doi.org/10.1016/0008-6215(91)80129-B

Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993

Karlin, S.; Altschul, S. F.
Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877
https://doi.org/10.1073/pnas.90.12.5873

Use of Synthetic Oligodeoxyribonucleotides for the Isolation of Specific Cloned DNA Sequences
book, January 1981

Suggs, Sidney V.; Hirose, Tadaaki; Miyake, Tetsuo
Developmental Biology Using Purified Genes
https://doi.org/10.1016/B978-0-12-137420-4.50059-0

Intercontinental spread of a genetically distinctive complex of clones of Neisseria meningitidis causing epidemic disease.
journal, July 1986

Caugant, D. A.; Froholm, L. O.; Bovre, K.
Proceedings of the National Academy of Sciences, Vol. 83, Issue 13
https://doi.org/10.1073/pnas.83.13.4927

Genetic organization of an Acinetobacter baumannii chromosomal region harbouring genes related to siderophore biosynthesis and transport
journal, May 2003

Dorsey, Caleb W.; Tolmasky, Marcelo E.; Crosa, Jorge H.
Microbiology, Vol. 149, Issue 5
https://doi.org/10.1099/mic.0.26204-0

Serine Acetyltransferase from Escherichia coli Is a Dimer of Trimers
journal, January 2000

Hindson, V. John; Moody, Peter C. E.; Rowe, Arthur J.
Journal of Biological Chemistry, Vol. 275, Issue 1
https://doi.org/10.1074/jbc.275.1.461

Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia
journal, December 1985

Saiki, R.; Scharf, S.; Faloona, F.
Science, Vol. 230, Issue 4732
https://doi.org/10.1126/science.2999980

Similar Records in DOE Patents and OSTI.GOV collections:

Method to produce a polysaccharide gel by increasing the pH of the polysaccharide

Patent Mirsiaghi, Mona; Sundstrom, Eric R.; Tanjore, Deepti; ...

The present invention provides for a method of producing a polysaccharide gel comprising: (a) culturing or growing a cyanobacterium or a mixture of cyanobacteria species in a medium under conditions such that the cyanobacterium or the mixture of cyanobacteria species produces a polysaccharide or a mixture of polysaccharides, (b) optionally separating the polysaccharide or the mixture of polysaccharides from the medium and/or the cyanobacterium or the mixture of cyanobacteria species, and (c) increasing the pH of the polysaccharide or the mixture of polysaccharides such that the polysaccharide or the mixture of polysaccharides forms a gel.
Full Text Available
Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)

Patent Durrett, Timothy; Ohlrogge, John; Pollard, Michael

The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils andmore » « less
Full Text Available
Methods of saccharification of polysaccharides in plants

Patent Howard, John; Fake, Gina

Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional .beta.-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously appliedmore » « less
Full Text Available
Methods of saccharification of polysaccharides in plants

Patent Howard, John; Fake, Gina

Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional .beta.-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously appliedmore » « less
Full Text Available
Method of creating industrial streptomyces with capability to grow on cellulosic polysaccharide substrates

Patent Fox, Brian Grant; Stankey, Robert Joseph; Currie, Cameron Robert; ...

A heterologous gene cassette useful for creating Streptomyces species with enhanced capability of growing on a cellulosic polysaccharide substrate, wherein the cassette comprises at least two members of the following categories: a) a GH6 gene, b) an AA10 gene, c) a GH48 gene, d) a GH5 gene and e) either (i) a GH9 gene, (ii) a GH9 gene and a GH12 gene, or (iii) a GH12 gene is disclosed.
Full Text Available

Similar Records

Title: Neisseria meningitidis serogroup A capsular polysaccharide acetyltransferase, methods and compositions

Abstract

Citation Formats

Possible dissociation of the heparin-binding and mitogenic activities of heparin-binding (acidic fibroblast) growth factor-1 from its receptor-binding activities by site-directed mutagenesis of a single lysine residue. journal, November 1990

Molecular characterization of the O-acetyl transferase gene of converting bacteriophage SF6 that adds group antigen 6 to Shigella flexneri journal, January 1991

Structure, assembly and regulation of expression of capsules in Escherichia coli journal, March 1999

Nodulation protein NodL of Rhizobium leguminosarum O-acetylates lipo-oligosaccharides, chitin fragments and N-acetylglucosamine in vitro journal, February 1994

Characterization of epidemic Neisseria meningitidis serogroup C strains in several Brazilian states. journal, January 1994

The Changing Epidemiology of Invasive Meningococcal Disease in Canada, 1985 Through 1992: Emergence of a Virulent Clone of Neisseria meningitidis journal, February 1995

Relation between structure and immunologic properties of the Vi capsular polysaccharide. journal, January 1991

Identification of a gene essential for O-acetylation of the Staphylococcus aureus type 5 capsular polysaccharide journal, January 1998

The Biochemistry and Genetics of Capsular Polysaccharide Production in Bacteria journal, October 1996

Powers and Pitfalls in Sequence Analysis: The 70% Hurdle journal, April 2000

Safety and immunogenicity of a new Neisseria meningitidis serogroup C-tetanus toxoid conjugate vaccine in healthy adults journal, November 1999

Epidemic Meningococcal Disease in Nairobi, Kenya, 1989 journal, August 1992

Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. journal, March 1990

Naturally-acquired immunity to Neisseria meningitidis group A journal, January 2005

Genetic and biochemical evidence of a Campylobacter jejuni capsular polysaccharide that accounts for Penner serotype specificity: Genetics and biochemistry of C. jejuni LPS biosynthesis journal, February 2000

Genetics of capsule O-acetylation in serogroup C, W-135 and Y meningococci: Capsule O-acetylation in meningococci journal, November 2003

Antigenic Determinants of Staphylococcus aureus Type 5 and Type 8 Capsular Polysaccharide Vaccines journal, October 1998

Full 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharides from Neisseria meningitidis used in vaccine production journal, December 1996

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs journal, September 1997

INFECTIOUS DISEASE: An Ill Wind, Bringing Meningitis journal, June 2008

Structural and Mechanistic Studies of Galactoside Acetyltransferase, the Escherichia coli LacA Gene Product journal, November 1995

Endotoxin of Neisseria meningitidis Composed Only of Intact Lipid A: Inactivation of the Meningococcal 3-Deoxy-d-Manno-Octulosonic Acid Transferase journal, May 2002

Kinetic and hydrodynamic studies of the NodL O-acetyl transferase of Rhizobium leguminosarum: a random-order ternary complex mechanism for acetyl transfer by a roughly spherical trimeric protein journal, June 2000

O -Acetylation status of the capsular polysaccharides of serogroup Y and W135 meningococci isolated in the UK journal, January 2002

Host Recognition of Bacterial Muramyl Dipeptide Mediated through NOD2: IMPLICATIONS FOR CROHN′S DISEASE journal, January 2003

Colorimetric Method for Determination of Sugars and Related Substances journal, March 1956

Interactions of VirB9, -10, and -11 with the membrane fraction of Agrobacterium tumefaciens: solubility studies provide evidence for tight associations. journal, January 1995

Antibody Responses to Capsular Polysaccharide Backbone and O-Acetate Side Groups of Streptococcus pneumoniae Type 9V in Humans and Rhesus Macaques journal, August 1998

Resistance to nodulation of cv. Afghanistan peas is overcome by nodX , which mediates an O-acetylation of the Rhizobium leguminosarum lipo-oligosaccharide nodulation factor journal, October 1993

Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile "Caldocellum saccharolyticum". journal, January 1990

Structures of the Capsular Polysaccharides of Neisseria meningitidis as Determined by 13C-Nuclear Magnetic Resonance Spectroscopy journal, August 1977

Genetic Diversity of Penicillin-Resistant Neisseria meningitidis journal, July 1992

Human Immunity to the Meningococcus journal, June 1969

The serine acetyltransferase from Escherichia coli: Over-expression, purification and preliminary crystallographic analysis journal, December 1990

Evaluation of De-O-Acetylated Meningococcal C Polysaccharide-Tetanus Toxoid Conjugate Vaccine in Infancy: Reactogenicity, Immunogenicity, Immunologic Priming, and Bactericidal Activity against O-Acetylated and De-O-Acetylated Serogroup C Strains journal, April 2001

Risk of group A meningococcal disease: bacterial interference and cross-reactive bacteria among mucosal flora. journal, January 1985

Development and Evaluation of an Improved Mouse Model of Meningococcal Colonization journal, April 2003

Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis journal, January 1981

Studies on transformation of Escherichia coli with plasmids journal, June 1983

A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module: Modular cinnamoyl esterase from P. funiculosum journal, December 2000

Serogroup C meningococcal outbreaks in the United States. An emerging threat journal, February 1995

Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide. journal, March 1979

Meningococcal vaccines—present and future journal, January 1991

Basic local alignment search tool journal, October 1990

Use and validation of NMR assays for the identity and O-acetyl content of capsular polysaccharides from Neisseria meningitidis used in vaccine manufacture journal, November 2002

Effect of O Acetylation of Neisseria meningitidis Serogroup A Capsular Polysaccharide on Development of Functional Immune Responses journal, July 2002

The (α2→8)-Linked Polysialic Acid Capsule and Lipooligosaccharide Structure Both Contribute to the Ability of Serogroup B Neisseria meningitidis To Resist the Bactericidal Activity of Normal Human Serum journal, December 1998

A point mutation in the active site of O-acetyltransferase results in modified lipopolysaccharide but does not influence virulence journal, January 2001

Mutant Analysis and Cellular Localization of the AlgI, AlgJ, and AlgF Proteins Required for O Acetylation of Alginate in Pseudomonas aeruginosa journal, June 2002

The human oestrogen receptor functions in yeast journal, July 1988

Synthesis of deoxyoligonucleotides on a polymer support journal, June 1981

Human bactericidal antibody response to meningococcal outer membrane protein vaccines journal, January 1987

Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus. journal, January 1997

Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern. journal, January 1987

Complement Activation and Formation of the Membrane Attack Complex on Serogroup B Neisseria meningitidis in the Presence or Absence of Serum Bactericidal Activity journal, July 2002

Structural identification of the iipo-chitin oligosaccharide nodulation signals of Rhizobium loti journal, February 1995

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors journal, January 1985

Directed Mutagenesis journal, December 1981

Interaction of Neisseria meningitidis with Human Nasopharyngeal Mucosa: Attachment and Entry into Columnar Epithelial Cells journal, September 1983

Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities. journal, March 1988

Neisseria meningitidis Strains Isolated from Invasive Infections in France (1999-2002): Phenotypes and Antibiotic Susceptibility Patterns journal, October 2003

Rhizobium fredii and Rhizobium meliloti produce 3-deoxy-D-manno-2-octulosonic acid-containing polysaccharides that are structurally analogous to group II K antigens (capsular polysaccharides) found in Escherichia coli. journal, January 1993

Hitting Early, Epidemic Meningitis Ravages Nigeria and Niger journal, April 2009

A macrolide 3-O-acyltransferase gene from the midecamycin-producing species Streptomyces mycarofaciens. journal, January 1992

A novel highly unsaturated fatty acid moiety of lipo-oligosaccharide signals determines host specificity of Rhizobium journal, November 1991

Isolating "Uncultivable" Microorganisms in Pure Culture in a Simulated Natural Environment journal, May 2002

L-Cysteine Biosynthesis in Escherichia coli: Nucleotide Sequence and Expression of the Serine Acetyltransferase (cysE) Gene from the Wild-type and a Cysteine-excreting Mutant journal, March 1987

The Neisseria meningitidis Serogroup A Capsular Polysaccharide O-3 and O-4 Acetyltransferase journal, August 2004

Engineering proteins for purification journal, January 1990

Induction and repression of outer membrane proteins by anaerobic growth of Neisseria gonorrhoeae. journal, January 1987

Detection of Conserved N -Linked Glycans and Phase-variable Lipooligosaccharides and Capsules from Campylobacter Cells by Mass Spectrometry and High Resolution Magic Angle Spinning NMR Spectroscopy journal, April 2003

Practical Methods in Molecular Biology book, January 1981

Deciphering the message in protein sequences: tolerance to amino acid substitutions journal, March 1990

Structural determination of the polysaccharide antigens of Neisseria meningitidis serogroups Y, W-135, and BO journal, January 1976

Characterization of the Gene Cassette Required for Biosynthesis of the (α1→6)-LinkedN-Acetyl-d-Mannosamine-1-Phosphate Capsule of Serogroup A Neisseria meningitidis journal, March 1998

Chemical methods for the analysis of sulphated galactans from red algae journal, March 1991

Applications and statistics for multiple high-scoring segments in molecular sequences. journal, June 1993

Possible dissociation of the heparin-binding and mitogenic activities of heparin-binding (acidic fibroblast) growth factor-1 from its receptor-binding activities by site-directed mutagenesis of a single lysine residue.
journal, November 1990

Molecular characterization of the O-acetyl transferase gene of converting bacteriophage SF6 that adds group antigen 6 to Shigella flexneri
journal, January 1991

Structure, assembly and regulation of expression of capsules in Escherichia coli
journal, March 1999

Nodulation protein NodL of Rhizobium leguminosarum O-acetylates lipo-oligosaccharides, chitin fragments and N-acetylglucosamine in vitro
journal, February 1994

Characterization of epidemic Neisseria meningitidis serogroup C strains in several Brazilian states.
journal, January 1994

The Changing Epidemiology of Invasive Meningococcal Disease in Canada, 1985 Through 1992: Emergence of a Virulent Clone of Neisseria meningitidis
journal, February 1995

Relation between structure and immunologic properties of the Vi capsular polysaccharide.
journal, January 1991

Identification of a gene essential for O-acetylation of the Staphylococcus aureus type 5 capsular polysaccharide
journal, January 1998

The Biochemistry and Genetics of Capsular Polysaccharide Production in Bacteria
journal, October 1996

Powers and Pitfalls in Sequence Analysis: The 70% Hurdle
journal, April 2000

Safety and immunogenicity of a new Neisseria meningitidis serogroup C-tetanus toxoid conjugate vaccine in healthy adults
journal, November 1999

Epidemic Meningococcal Disease in Nairobi, Kenya, 1989
journal, August 1992

Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes.
journal, March 1990

Naturally-acquired immunity to Neisseria meningitidis group A
journal, January 2005

Genetic and biochemical evidence of a Campylobacter jejuni capsular polysaccharide that accounts for Penner serotype specificity: Genetics and biochemistry of C. jejuni LPS biosynthesis
journal, February 2000

Genetics of capsule O-acetylation in serogroup C, W-135 and Y meningococci: Capsule O-acetylation in meningococci
journal, November 2003

Antigenic Determinants of Staphylococcus aureus Type 5 and Type 8 Capsular Polysaccharide Vaccines
journal, October 1998

Full 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharides from Neisseria meningitidis used in vaccine production
journal, December 1996

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

INFECTIOUS DISEASE: An Ill Wind, Bringing Meningitis
journal, June 2008

Structural and Mechanistic Studies of Galactoside Acetyltransferase, the Escherichia coli LacA Gene Product
journal, November 1995

Endotoxin of Neisseria meningitidis Composed Only of Intact Lipid A: Inactivation of the Meningococcal 3-Deoxy-d-Manno-Octulosonic Acid Transferase
journal, May 2002

Kinetic and hydrodynamic studies of the NodL O-acetyl transferase of Rhizobium leguminosarum: a random-order ternary complex mechanism for acetyl transfer by a roughly spherical trimeric protein
journal, June 2000

O -Acetylation status of the capsular polysaccharides of serogroup Y and W135 meningococci isolated in the UK
journal, January 2002

Host Recognition of Bacterial Muramyl Dipeptide Mediated through NOD2: IMPLICATIONS FOR CROHN′S DISEASE
journal, January 2003

Colorimetric Method for Determination of Sugars and Related Substances
journal, March 1956

Interactions of VirB9, -10, and -11 with the membrane fraction of Agrobacterium tumefaciens: solubility studies provide evidence for tight associations.
journal, January 1995

Antibody Responses to Capsular Polysaccharide Backbone and O-Acetate Side Groups of Streptococcus pneumoniae Type 9V in Humans and Rhesus Macaques
journal, August 1998

Resistance to nodulation of cv. Afghanistan peas is overcome by nodX , which mediates an O-acetylation of the Rhizobium leguminosarum lipo-oligosaccharide nodulation factor
journal, October 1993

Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile "Caldocellum saccharolyticum".
journal, January 1990

Structures of the Capsular Polysaccharides of Neisseria meningitidis as Determined by 13C-Nuclear Magnetic Resonance Spectroscopy
journal, August 1977

Genetic Diversity of Penicillin-Resistant Neisseria meningitidis
journal, July 1992

Human Immunity to the Meningococcus
journal, June 1969

The serine acetyltransferase from Escherichia coli: Over-expression, purification and preliminary crystallographic analysis
journal, December 1990

Evaluation of De-O-Acetylated Meningococcal C Polysaccharide-Tetanus Toxoid Conjugate Vaccine in Infancy: Reactogenicity, Immunogenicity, Immunologic Priming, and Bactericidal Activity against O-Acetylated and De-O-Acetylated Serogroup C Strains
journal, April 2001

Risk of group A meningococcal disease: bacterial interference and cross-reactive bacteria among mucosal flora.
journal, January 1985

Development and Evaluation of an Improved Mouse Model of Meningococcal Colonization
journal, April 2003

Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
journal, January 1981

Studies on transformation of Escherichia coli with plasmids
journal, June 1983

A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module: Modular cinnamoyl esterase from P. funiculosum
journal, December 2000

Serogroup C meningococcal outbreaks in the United States. An emerging threat
journal, February 1995

Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide.
journal, March 1979

Meningococcal vaccines—present and future
journal, January 1991

Basic local alignment search tool
journal, October 1990

Use and validation of NMR assays for the identity and O-acetyl content of capsular polysaccharides from Neisseria meningitidis used in vaccine manufacture
journal, November 2002

Effect of O Acetylation of Neisseria meningitidis Serogroup A Capsular Polysaccharide on Development of Functional Immune Responses
journal, July 2002

The (α2→8)-Linked Polysialic Acid Capsule and Lipooligosaccharide Structure Both Contribute to the Ability of Serogroup B Neisseria meningitidis To Resist the Bactericidal Activity of Normal Human Serum
journal, December 1998

A point mutation in the active site of O-acetyltransferase results in modified lipopolysaccharide but does not influence virulence
journal, January 2001

Mutant Analysis and Cellular Localization of the AlgI, AlgJ, and AlgF Proteins Required for O Acetylation of Alginate in Pseudomonas aeruginosa
journal, June 2002

The human oestrogen receptor functions in yeast
journal, July 1988

Synthesis of deoxyoligonucleotides on a polymer support
journal, June 1981

Human bactericidal antibody response to meningococcal outer membrane protein vaccines
journal, January 1987

Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus.
journal, January 1997

Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern.
journal, January 1987

Complement Activation and Formation of the Membrane Attack Complex on Serogroup B Neisseria meningitidis in the Presence or Absence of Serum Bactericidal Activity
journal, July 2002

Structural identification of the iipo-chitin oligosaccharide nodulation signals of Rhizobium loti
journal, February 1995

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
journal, January 1985

Directed Mutagenesis
journal, December 1981

Interaction of Neisseria meningitidis with Human Nasopharyngeal Mucosa: Attachment and Entry into Columnar Epithelial Cells
journal, September 1983

Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988

Neisseria meningitidis Strains Isolated from Invasive Infections in France (1999-2002): Phenotypes and Antibiotic Susceptibility Patterns
journal, October 2003

Rhizobium fredii and Rhizobium meliloti produce 3-deoxy-D-manno-2-octulosonic acid-containing polysaccharides that are structurally analogous to group II K antigens (capsular polysaccharides) found in Escherichia coli.
journal, January 1993

Hitting Early, Epidemic Meningitis Ravages Nigeria and Niger
journal, April 2009

A macrolide 3-O-acyltransferase gene from the midecamycin-producing species Streptomyces mycarofaciens.
journal, January 1992

A novel highly unsaturated fatty acid moiety of lipo-oligosaccharide signals determines host specificity of Rhizobium
journal, November 1991

Isolating "Uncultivable" Microorganisms in Pure Culture in a Simulated Natural Environment
journal, May 2002

L-Cysteine Biosynthesis in Escherichia coli: Nucleotide Sequence and Expression of the Serine Acetyltransferase (cysE) Gene from the Wild-type and a Cysteine-excreting Mutant
journal, March 1987

The Neisseria meningitidis Serogroup A Capsular Polysaccharide O-3 and O-4 Acetyltransferase
journal, August 2004

Engineering proteins for purification
journal, January 1990

Induction and repression of outer membrane proteins by anaerobic growth of Neisseria gonorrhoeae.
journal, January 1987

Detection of Conserved N -Linked Glycans and Phase-variable Lipooligosaccharides and Capsules from Campylobacter Cells by Mass Spectrometry and High Resolution Magic Angle Spinning NMR Spectroscopy
journal, April 2003

Practical Methods in Molecular Biology
book, January 1981

Deciphering the message in protein sequences: tolerance to amino acid substitutions
journal, March 1990

Structural determination of the polysaccharide antigens of Neisseria meningitidis serogroups Y, W-135, and BO
journal, January 1976

Characterization of the Gene Cassette Required for Biosynthesis of the (α1→6)-LinkedN-Acetyl-d-Mannosamine-1-Phosphate Capsule of Serogroup A Neisseria meningitidis
journal, March 1998

Chemical methods for the analysis of sulphated galactans from red algae
journal, March 1991

Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993

Use of Synthetic Oligodeoxyribonucleotides for the Isolation of Specific Cloned DNA Sequences
book, January 1981

Intercontinental spread of a genetically distinctive complex of clones of Neisseria meningitidis causing epidemic disease.
journal, July 1986

Genetic organization of an Acinetobacter baumannii chromosomal region harbouring genes related to siderophore biosynthesis and transport
journal, May 2003