3-dimensional imaging at nanometer resolutions
Abstract
An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.
- Inventors:
-
- Los Alamos, NM
- Santa Fe, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1014498
- Patent Number(s):
- 7675045
- Application Number:
- U S Patent Application 12/248,834
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- DOE Contract Number:
- AC52-06NA25396
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Werner, James H, Goodwin, Peter M, and Shreve, Andrew P. 3-dimensional imaging at nanometer resolutions. United States: N. p., 2010.
Web.
Werner, James H, Goodwin, Peter M, & Shreve, Andrew P. 3-dimensional imaging at nanometer resolutions. United States.
Werner, James H, Goodwin, Peter M, and Shreve, Andrew P. Tue .
"3-dimensional imaging at nanometer resolutions". United States. https://www.osti.gov/servlets/purl/1014498.
@article{osti_1014498,
title = {3-dimensional imaging at nanometer resolutions},
author = {Werner, James H and Goodwin, Peter M and Shreve, Andrew P},
abstractNote = {An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 09 00:00:00 EST 2010},
month = {Tue Mar 09 00:00:00 EST 2010}
}
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