Title: Rapid classification of biological components

Abstract

A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens of the surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

Inventors:

Thompson, Vicki S ^[1]; Barrett, Karen B ^[2]; Key, Diane E ^[1]

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+ Show Inventor Affiliations

1. Idaho Falls, ID
2. Meridian, ID

Issue Date:

Tue Mar 23 00:00:00 EDT 2010

Research Org.:

Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1014467

Patent Number(s):

7682798

Application Number:

U S Patent Application 11/101,254

Assignee:

Battelle Energy Alliance, LLC (Idaho Falls, ID)

Patent Classifications (CPCs):

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/543 - with an insoluble carrier for immobilising immunochemicals
G01N33/94 - involving narcotics {or drugs or pharmaceuticals, neurotransmitters or associated receptors}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S435/971 - Capture of complex after antigen-antibody reaction
Y10S435/973 - Simultaneous determination of more than one analyte
Y10S435/975 - Kit

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DOE Contract Number:  

AC07-94ID13223; AC07-99ID13727; AC07-05ID14517

Resource Type:

Patent

Country of Publication:

United States

Language:

English