1741 K
26 pp.
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TitleThe Path of Carbon in Photosynthesis XV. Ribulose and Sedoheptulose
Author(s)Benson, A. A.; Bassham, J. A.; Calvin, M.; Hall, A. G.; Hirsch, H.; Kawaguchi, S.; Lynch, V.; Tolbert, N. E.
Publication DateJanuary 1952
Report NumberUCRL-1609
Unique IdentifierACC0318
Other NumbersOSTI ID: 4409746; OSTI ID: 915054
Research OrgUniversity of California Radiation Laboratory, Berkeley, CA; Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US) [LBL][LBNL]
Contract NoW-7405-eng-48; DE-AC02-05CH11231
Sponsoring OrgU.S. Atomic Energy Commission (AEC)
Subject14 Solar Energy; Ketones; Carbon Cycle; Photosynthesis; Ribulose; Chemical Reaction Kinetics
KeywordsChemistry; Biochemistry; Carbohydrates; Measured Values; Phosphates; Sugars; Tracer Techniques; Ribulose, Sedoheptulose
Related Web PagesMelvin Calvin and Carbon in Photosynthesis
AbstractThe intermediates of carbon dioxide reduction by plants include phosphorylated derivatives of hydroxy acids and sugars. Their identification became possible when the use of labeled carbon dioxide permitted discrimination between the earliest products and the many other components of photosynthetic tissues. A number of compounds were identified by virtue of the chemical and physical properties of the radioactive compounds in tracer amounts and by direct comparison of these properties with those of suspected known metabolic intermediates. It became apparent that several labeled compounds found in short exposures to radioactive carbon dioxide were not substances previously identified as metabolic intermediates. Two phosphate esters in particular were observed in the products of the first few seconds of steady-state photosynthesis by all the photosynthetic microorganisms and higher plants examined in this laboratory. These esters have been isolated by paper chromatography in tracer quantities and enzymatically hydrolyzed to give two sugars, ribulose and sedoheptulose. This paper contains a description of the chemical identification of these sugars and some observations and suggestions regarding the function of their esters. The general importance of these compounds in photosynthesis was summarized before their identification. The products of photosynthesis with C{sup 14}O{sub 2} by each plant included phosphate esters of the same two then unknown compounds in addition to those of the expected glucose, fructose, dihydroxyacetone and glyceric acid. As the time of steady-state photosynthesis in C{sup 14}O{sub 2} decreased, the fractions of total fixed radiocarbon in the esters of the two unidentified compounds increased.
1741 K
26 pp.
View Document 

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