1798 K
57 pp.
 
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TitleOccurrence and Characteristics of a Rapid Exchange of Phosphate Oxygens Catalyzed by Sarcoplasmic Reticulum Vesicles
Author(s)Kanazawa, T.; Boyer, P. D.
Publication Date1972
Report NumberUCLA--34-P-102-35
Unique IdentifierACC0192
Other NumbersOSTI ID: 4473783
Research OrgUniversity of California, Los Angeles (USA)
Contract NoAT(04-3)-34
Sponsoring OrgU.S. Atomic Energy Commission (AEC)
Subject06 Biomed; 07 Chemistry; Animal Cells -- Respiration; ATP-ASE; Biochemical Reaction Kinetics; Calcium; Catalysts; Endoplasmic Reticulum; Magnesium; Metabolism; Muscles; Oxygen; Phosphates; Rabbits; Water
Related Web PagesPaul D. Boyer, Adenosine Triphosphate [ATP], and the Binding Change Mechanism
AbstractSarcoplasmic reticulum vesicles isolated from skeletal muscle actively take up Ca{sup ++} from the medium in the presence of Mg{sup ++} and ATP. This transport is coupled to ATP hydrolysis catalyzed by membrane-bound Ca{sup++}, Mg{sup ++}-ATPase which is activated by concurrent presence of Ca{sup ++} and Mg{sup ++}. Considerable informations have accumulated that give insight into the ATPase and its coupling to the calcium transport. The hydrolysis of ATP by this enzyme occurs through a phosphorylated intermediate. Formation and decomposition of the intermediate show vectorial requirements for Ca{sup ++} and Mg{sup ++}, suggesting an intimate involvement of the intermediate in the transport process. ATP synthesis from P{sub i} and ADP coupled to outflow of Ca{sup ++} from sarcoplasmic reticulum vesicles has recently been demonstrated. This indicates the reversibility of the entire process of calcium transport in sarcoplasmic reticulum vesicles.
1798 K
57 pp.
 
View Document 
  


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