45 pp.
45 pp.
| 1475 K 45 pp. | |
| View Document | ||
| Title | Occurrence and Characteristics of {sup 18}O-exchange Reactions Catalyzed By Sodium- and Potassium-dependent Adenosine Triphosphatases | |
| Author(s) | Dahms, A. S.; Boyer, P. D. | |
| Publication Date | 1972 | |
| Report Number | UCLA--34-P-102-36 | |
| Unique Identifier | ACC0191 | |
| Other Numbers | OSTI ID: 4485348 | |
| Research Org | University of California, Los Angeles (USA) | |
| Contract No | AT(04-3)-34 | |
| Sponsoring Org | U.S. Atomic Energy Commission (AEC) | |
| Subject | N40230 -- Chemistry -- Inorganic, Organic, & Physical Chemistry -- Isotope Exchange & Isotope Separation; Oxygen 18 -- Isotopic Exchange; ATP-ASE; Catalysis; Chemical Reaction Kinetics; Potassium Compounds; Sodium Compounds; Water | |
| Related Web Pages | Paul D. Boyer, Adenosine Triphosphate [ATP], and the Binding Change Mechanism | |
| Abstract | Microsomal preparations of the (Na{sup +},K{sup +}-ATPase from porcine outer medulla and electroplax catalyzed a rapid Mg{sup 2+}- and K{sup +}-dependent exchange of water oxygens with inorganic phosphate in the absence of ATP or ADP. Exchange activity was unaffected by uncouplers and inhibitors of oxidative phosphorylation but was inhibited by Na{sup +}, ouabain, N,N'-dicyclohexylcarbodiimide, or p-mercuribenzoate. No nucleotide requirement for the exchange could be demonstrated. Addition of ATP to Na{sup +}-inhibited system resulted in an exchange of oxygens of medium P{sub i} concomitant with ATP hydrolysis. This ATP-induced exchange amounted to 1.5 oxygen atoms per P{sub i} released. Neither ADP nor adenylyl methylene diphosphonate would serve to activate the exchange. | |
| 1475 K 45 pp. | |
| View Document | ||
