Lack of evidence for low-LET radiation induced bystander response in normal human fibroblasts and colon carcinoma cells
Purpose: To investigate radiation induced bystander responses and to determine the role of gap junction intercellular communication and the radiation environment in propagating this response. Materials and Methods: We use medium transfer and targeted irradiation to examine radiation induced bystander effects in primary human fibroblast (AG1522) and human colon carcinoma (RKO36) cells. We examined the effect of variables such as gap junction intercellular communication, linear energy transfer (LET), and the role of the radiation environment in non-targeted responses. Endpoints included clonogenic survival, micronucleus formation and foci formation at histone 2AX over doses ranging from 10 to 100 cGy. Results: The results show no evidence of a low-LET radiation induced bystander response for the endpoints of clonogenic survival and induction of DNA damage. Nor do we see evidence of a high-LET, Fe ion radiation (1 GeV/n) induced bystander effect. However, direct comparison for 3.2 MeV α-particle exposures showed a statistically significant medium transfer bystander effect for this high-LET radiation. Conclusions: From our results, it is evident that there are many confounding factors influencing bystander responses as reported in the literature. Our observations reflect the inherent variability in biological systems and the difficulties in extrapolating from in vitro models to radiation risks in humans.
- Research Organization:
- University of Maryland, Baltimore, MD
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- FG02-05ER64082
- OSTI ID:
- 960295
- Report Number(s):
- DOE\ER\64082- Final Report
- Country of Publication:
- United States
- Language:
- English
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Lack of evidence for low-LET radiation induced bystander response in normal human fibroblasts and colon carcinoma cells