Bioelectrochemical Immunoassay of Polychlorinated Biphenyl

Journal Article · Tue Apr 01 00:00:00 EDT 2008 · Analytica Chimica Acta, 612:23-28

DOI:https://doi.org/10.1016/j.aca.2008.01.080· OSTI ID:927705

Lin, Ying-Ying; Liu, Guodong; Wai, Chien M; Lin, Yuehe

A simple, rapid, and highly sensitive bioelectrochemical immunoassay method based on magnetic beads (MBs) and disposable screen-printed electrodes (SPE) has been developed to detect polychlorinated biphenyls (PCBs). The principle of this bioassay is based on a direct competitive enzyme-linked immunosorbent assay using PCB-antibody-coated MBs and horseradish peroxidase (HRP)-labeled PCB (HRP-PCB). A magnetic process platform was used to mix and shake the samples during the immunoreactions and to separate free and unbound reagents after the liquid-phase competitive immunoreactions among PCB-antibody-coated MBs, PCB analyte, and HRP-PCB. After a complete immunoassay, the HRP tracers attached to MBs were transferred to a substrate solution containing o-aminophenol and hydrogen peroxide for electrochemical detection. The different parameters, including the amount of HRP-PCB conjugates, immunoreaction time, and the concentration of substrate that governs the analytical performance of the immunoassay have been studied in detail and optimized. The detection limit of 5 pg mL-1 was obtained under optimum experimental conditions. The performance of this bioelectrochemical immunoassay was successfully evaluated with untreated river water spiked with PCBs, and the results were validated by commercial PCB enzyme-linked immunosorbent assay kit, indicating that this convenient and sensitive technique offers great promise for decentralized environmental application and trace PCBs monitoring.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 927705
• Report Number(s): PNNL-SA-59125; ACACAM; 24822; TRN: US0804796
• Journal Information: Analytica Chimica Acta, 612:23-28, Vol. 612; ISSN 0003-2670
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS
BIOASSAY
DETECTION
ELECTRODES
HYDROGEN PEROXIDE
IMMUNOASSAY
MONITORING
PERFORMANCE
PEROXIDASES
POLYCHLORINATED BIPHENYLS
RIVERS
SENSITIVITY
SOLAR PROTONS
SUBSTRATES
WATER
Immunoassay
PCB
Environmental Molecular Sciences Laboratory