Genome Sequence and Analysis of the Soil Cellulolytic ActinomyceteThermobifida fusca
Thermobifida fusca is a moderately thermophilic soilbacterium that belongs to Actinobacteria. It is a major degrader of plantcell walls and has been used as a model organism for the study ofsecreted, thermostable cellulases. The complete genome sequence showedthat T. fusca has a single circular chromosome of 3642249 bp predicted toencode 3117 proteins and 65 RNA species with a coding density of 85percent. Genome analysis revealed the existence of 29 putative glycosidehydrolases in addition to the previously identified cellulases andxylanases. The glycosyl hydrolases include enzymes predicted to exhibitmainly dextran/starch and xylan degrading functions. T. fusca possessestwo protein secretion systems: the sec general secretion system and thetwin-arginine translocation system. Several of the secreted cellulaseshave sequence signatures indicating their secretion may be mediated bythe twin-arginine translocation system. T. fusca has extensive transportsystems for import of carbohydrates coupled to transcriptional regulatorscontrolling the expression of the transporters and glycosylhydrolases. Inaddition to providing an overview of the physiology of a soilactinomycete, this study presents insights on the transcriptionalregulation and secretion of cellulases which may facilitate theindustrial exploitation of these systems.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Director. Office of Science. Biological andEnvironmental Research
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 917048
- Report Number(s):
- LBNL-60434; JOBAAY; R&D Project: 626869; BnR: KP1103010; TRN: US200816%%251
- Journal Information:
- The Journal of Bacteriology, Vol. 189, Issue 6; Related Information: Journal Publication Date: 03/2007; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
Similar Records
Genome Sequence and Analysis of the Soil Cellulolytic Actinomycete Thermobifida fusca YX
Final Technical Report