Method of saccharifying cellulose

Patent · Tue Sep 10 00:00:00 EDT 1985

OSTI ID:865590

Johnson, Eric A ^[1]; Demain, Arnold L ^[2]; Madia, Ashwin ^[3]

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1. Brookline, MA
2. Wellesley, MA
3. Decatur, IL

A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.

• Research Organization: Midwest Research Institute, Kansas City, MO (United States)
• DOE Contract Number: EG-77-C-02-4198; XR-9-8109-1
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4540664
• Application Number: 06/494,482
• OSTI ID: 865590
• Country of Publication: United States
• Language: English

Related Subjects

method
saccharifying
cellulose
incubation
cellulase
clostridium
thermocellum
broth
containing
efficacious
amount
reducing
agent
parameters
advantageously
controlled
stimulate
saccharification
concentration
alkaline
earth
salts
ph
temperature
duration
native
crystalline
found
cotton
completely
saccharified
crystalline cellulose
alkaline earth
reducing agent
earth salts
saccharifying cellulose
clostridium thermocellum
/435/